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AB273247

Rabbit Angiopoietin-1 ELISA Kit

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Rabbit Angiopoietin-1 ELISA Kit is a Sandwich ELISA for the measurement of Rabbit Angiopoietin-1 in Rabbit in Biofluids, Cell Culture Media samples.
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Sandwich ELISA - Rabbit Angiopoietin-1 ELISA Kit (AB273247)
  • sELISA

Supplier Data

Sandwich ELISA - Rabbit Angiopoietin-1 ELISA Kit (AB273247)

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Rabbit

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.1 ng/mL

Range

0.102 - 25 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Rabbit Angiopoietin-1 ELISA Kit (ab273247) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of rabbit Angiopoietin-1 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for rabbit Angiopoietin-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Angiopoietin-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-rabbit Angiopoietin-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Angiopoietin-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "75 - 140 %", "average": "= 106.6" }, { "sample": "Plasma", "range": "67 - 82 %", "average": "= 71.85" }, { "sample": "Cell culture media", "range": "67 - 109 %", "average": "= 85.4" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273247-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273247-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB273247-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rabbit Angiopoietin-1 Standard (Lyophilized)", "number":"AB273247-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Rabbit Angiopoietin-1 Antibody", "number":"AB273247-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Rabbit Angiopoietin-1 coated Microplate (12 x 8 wells)", "number":"AB273247-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB273247-CMP06", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273247-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273247-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Angiopoietin 1 also known as Ang1 or Angpt1 is a protein important to the vascular system with a molecular mass of around 70 kDa. It is expressed widely in endothelial cells and fibroblasts. Angiopoietin 1 functions mechanically to bind the Tie2 receptor triggering signaling pathways that regulate blood vessel maturation and stability. This interaction is important for the development and maintenance of the blood vessel network.
Biological function summary

Angiopoietin 1 supports the structural integrity of blood vessels by interacting with the Tie2 receptor as part of a signaling complex. It helps prevent vascular leak and promotes endothelial cell survival migration and adhesion. By playing such roles Angiopoietin 1 is fundamental for maintaining vascular homeostasis and facilitating proper blood flow throughout the body. It also influences the immune response by modulating endothelial functions.

Pathways

Angiopoietin 1's involvement is most notable within the PI3K/AKT pathway and the MAPK pathway which are important for signaling cell survival and growth. These pathways work closely with other proteins such as VEGF (Vascular Endothelial Growth Factor) in angiogenesis and vascular remodeling. Angiopoietin 1 interacts with its related pathways to ensure that blood vessels remain functional and adaptive to physiological changes.

Angiopoietin 1 plays significant roles in cardiovascular diseases like atherosclerosis and diabetic vascular complications. Its dysregulation can lead to abnormalities in vascular maintenance and repair. There is also an association between Angiopoietin 1 and proteins like VEGF in cancer as both have roles in tumor angiogenesis. Understanding Angiopoietin 1's function could provide insights into therapeutic strategies for these conditions.
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Product protocols

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Target data

See full target information ANGPT1
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websiteProtocolBooklet
en
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Product promise

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