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AB273245

Rabbit CCL4 ELISA Kit

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Rabbit CCL4 ELISA Kit is a Sandwich ELISA for the measurement of Rabbit CCL4 in Rabbit in Biofluids, Cell Culture Media samples.

View Alternative Names

SCYA4, CCL4, C-C motif chemokine 4, Immune activation protein 2, Macrophage inflammatory protein 1-beta, Small-inducible cytokine A4, ACT-2, MIP-1-beta

1 Images
Sandwich ELISA - Rabbit CCL4 ELISA Kit (AB273245)
  • sELISA

Supplier Data

Sandwich ELISA - Rabbit CCL4 ELISA Kit (AB273245)

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Rabbit

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 24.6 pg/mL

Range

24.58 - 6000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Rabbit CCL4 ELISA Kit (ab273245) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of rabbit CCL4 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for rabbit CCL4 coated on a 96-well plate. Standards and samples are pipetted into the wells and CCL4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti- rabbit CCL4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CCL4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "89 - 102 %", "average": "= 94.8" }, { "sample": "Plasma", "range": "77 - 105 %", "average": "= 89.75" }, { "sample": "Cell culture media", "range": "98 - 134 %", "average": "= 109" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273245-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273245-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rabbit CCL4 Standard (Lyophilized)", "number":"AB273245-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273245-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Rabbit CCL4 Antibody", "number":"AB273245-CMP01", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB273245-CMP02", "productcode":"" }, { "size":"1 x 200 µL", "name":"700X HRP-Streptavidin Concentrate", "number":"AB273245-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Rabbit CCL4 coated Microplate (12 x 8 wells)", "number":"AB273245-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB273245-CMP07", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273245-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCL4 also known as MIP-1 beta or MIP-1b has a mechanical role as a chemokine involved in immune response regulation. The protein has a mass of about 7.8 kDa. It expresses in various tissues including leukocytes where it mainly functions as a chemoattractant. MIP-1 beta helps recruit immune cells like natural killer cells and monocytes to sites of inflammation or injury. Researchers frequently measure its levels using assays like MIP-1 beta ELISA or CCL4 ELISA.
Biological function summary

CCL4 is critical in immune system modulation. It coordinates leukocyte activation and trafficking contributing to inflammation and immune surveillance. It does not function in isolation but rather interacts with other chemokines and receptors. CCL4 alongside its sibling chemokines forms a dynamic network that ensures a balanced immune response. It connects to receptors such as CCR5 playing substantial roles in its activities.

Pathways

CCL4 participates in essential signaling cascades that mediate immune responses. It is notably part of the chemokine signaling pathway which is instrumental in directing cell movement. CCL4 also engages with proteins like CCR5 in the inflammatory and immune response pathways. This interaction enhances communication between cells required for effective pathogen defense.

CCL4 plays roles in immune-related conditions. It is strongly linked to inflammatory diseases such as rheumatoid arthritis and HIV infection. In rheumatoid arthritis CCL4 levels are often elevated correlating with inflammation severity. During HIV infection CCL4 interacts with proteins like CCR5 a co-receptor the virus uses to enter host cells. This involvement makes MIP-1 beta a target for studies seeking therapeutic interventions in these diseases.
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Product protocols

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Target data

Monokine with inflammatory and chemokinetic properties.
See full target information CCL4
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websiteProtocolBooklet
en
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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