JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB273248

Rabbit CXCL13 ELISA Kit (BCA-1)

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit CXCL13 ELISA Kit (BCA-1) is a Sandwich ELISA for the measurement of Rabbit CXCL13 (BCA-1) in Rabbit in Biofluids, Cell Culture Media samples.
1 Images
Sandwich ELISA - Rabbit CXCL13 ELISA Kit (BCA-1) (AB273248)
  • sELISA

Supplier Data

Sandwich ELISA - Rabbit CXCL13 ELISA Kit (BCA-1) (AB273248)

Rabbit CXCL13 ELISA Kit (BCA-1) standard curve.

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Rabbit

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.35 ng/mL

Range

0.328 - 80 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB284976

Anti-CXCL11 antibody [EPR21755-151] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-CXCL11 antibody [EPR21755-151] - BSA and Azide free (Capture) (AB284976)

  • 0
  • 0
Primary Antibodies

AB199043

Anti-CXCL13 antibody [EPR19259-147]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL13 antibody [EPR19259-147] (AB199043)

  • 5
  • 2
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Rabbit CXCL13 ELISA Kit (ab273248) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rabbit CXCL13 in serum (Rabbit CXCL13 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.

This assay employs an antibody specific for Rabbit CXCL13 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL13 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Rabbit CXCL13 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL13 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "69 - 100 %", "average": "= 82.44" }, { "sample": "Plasma", "range": "69 - 85 %", "average": "= 74.71" }, { "sample": "Cell culture media", "range": "67 - 132 %", "average": "= 89.88" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273248-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273248-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Rabbit CXCL13 (BCA-1) coated Microplate (12 x 8 wells)", "number":"AB273248-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB273248-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273248-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rabbit CXCL13 (BCA-1) Standard (Lyophilized)", "number":"AB273248-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB273248-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Rabbit CXCL13 (BCA-1) Antibody", "number":"AB273248-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273248-CMP03", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CXCL13 also known as B cell-attracting chemokine 1 (BCA-1) is a chemokine with a molecular weight of approximately 11 kDa. This protein plays an important mechanical role in the immune system by guiding the movement of B cells via chemotaxis. CXCL13 expression occurs mainly in secondary lymphoid tissues such as the spleen lymph nodes and mucosa-associated lymphoid tissue. Its expression is also noted in certain non-lymphoid tissues under inflammatory conditions.
Biological function summary

In the context of immune response and lymphoid tissue organization CXCL13 attracts B cells by interacting with the chemokine receptor CXCR5. These interactions support the formation of B-cell follicles essential for efficient immune responses. CXCL13 is not known to be part of a larger protein complex but instead works through its specific receptor-ligand interactions. Its role in guiding cell migration is vital for the development of secondary lymphoid structures.

Pathways

CXCL13 is significantly involved in the B-cell receptor signaling pathway. The chemokine facilitates the homing of B cells to follicles an important step in the adaptive immune response. CXCL13 closely associates with CXCR5 its specific receptor playing a part in establishing the spatial organization of lymphoid tissues. Additionally the chemokine influences the germinal center reaction an integral part of the immune pathway for antibody maturation.

CXCL13 levels have been linked to autoimmune diseases like rheumatoid arthritis and certain types of lymphomas. In rheumatoid arthritis increased CXCL13 expression correlates with disease severity related to its role in recruiting B cells that contribute to chronic inflammation. In lymphomas particularly those involving B cells CXCL13 supports the growth and survival of malignant cells. Its association with disease states often involves changes in CXCR5 expression or function contributing to disrupted B-cell localization and abnormal immune responses.
style
left-column

Product protocols

style
left-column

Target data

See full target information CXCL13
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com