Rabbit IL-17A ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Rabbit IL-17A in Rabbit in Plasma, Cell culture supernatant, Serum samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rabbit
- Range
- 0.205 - 50 ng/mL
- Sensitivity
- = 0.2 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
What's included?
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Rabbit IL-17A ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Rabbit IL-17A in Rabbit in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rabbit
- Range
- 0.205 - 50 ng/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.2 ng/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 12 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 118 | Range 96 - 145 % |
Sample type Plasma | Average % = 116.1 | Range 85 - 146 % |
Sample type Cell culture media | Average % = 130.5 | Range 116 - 143 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Rabbit IL-17A ELISA Kit (ab273240) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of rabbit IL-17A in serum (rabbit IL-17A concentration is low in normal serum and plasma samples, and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for rabbit IL-17A coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-17A present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti- rabbit IL-17A antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-17A bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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1 product image
Sandwich ELISA - Rabbit IL-17A ELISA Kit (ab273240)
This standard curve is for demonstration only. A standard curve must be run with each assay.
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Product protocols
- Visit the general protocols
- Visit troubleshooting
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