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AB277394

Rabbit IL-1RA ELISA Kit

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Rabbit IL-1RA ELISA Kit is a Sandwich ELISA for the measurement of Rabbit IL-1RA in Rabbit in Biofluids, Cell Culture Media samples.

View Alternative Names

IL1RA, IL1RN, Interleukin-1 receptor antagonist protein, IL-1RN, IL-1ra, IRAP, IL1 inhibitor

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ELISA - Rabbit IL-1RA ELISA Kit (AB277394)
  • ELISA

Supplier Data

ELISA - Rabbit IL-1RA ELISA Kit (AB277394)

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Rabbit

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.2 ng/mL

Range

0.205 - 50 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Rabbit IL-1RA ELISA Kit (ab277394) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rabbit IL-1RA in serum (Rabbit IL-1ra concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.

This assay employs an antibody specific for Rabbit IL-1RA coated on a 96-well plate. Standards and samples are pipetted into the wells and Rabbit IL-1RA present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Rabbit IL-1RA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Rabbit IL-1RA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "69 - 88 %", "average": "= 75.95" }, { "sample": "Plasma", "range": "70 - 95 %", "average": "= 83.97" }, { "sample": "Cell culture media", "range": "83 - 95 %", "average": "= 89.8" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277394-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277394-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277394-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Rabbit IL-1RA coated Microplate", "number":"AB277394-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB277394-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Rabbit IL-1 RA Antibody", "number":"AB277394-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rabbit IL-1RA standard protein (Lyophilized)", "number":"AB277394-CMP07", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB277394-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB277394-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277394-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Anti-inflammatory antagonist of interleukin-1 family of proinflammatory cytokines such as interleukin-1beta/IL1B and interleukin-1alpha/IL1A. Protects from immune dysregulation and uncontrolled systemic inflammation triggered by IL1 for a range of innate stimulatory agents such as pathogens.
See full target information IL1RN
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websiteProtocolBooklet
en
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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