Ramucirumab ELISA Kit is a Quantitative ELISA kit for the measurement of Ramucirumab in Human in Plasma, Serum samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Serum
- Assay type
- Quantitative
- Reactive species
- Human
- Range
- 20 - 600 ng/mL
- Sensitivity
- = 20 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain, such as isoform 2 and isoform 3, may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. Isoform 2 plays an important role as negative regulator of VEGFA- and VEGFC-mediated lymphangiogenesis by limiting the amount of free VEGFA and/or VEGFC and preventing their binding to FLT4. Modulates FLT1 and FLT4 signaling by forming heterodimers. Binding of vascular growth factors to isoform 1 leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, reorganization of the actin cytoskeleton and activation of PTK2/FAK1. Required for VEGFA-mediated induction of NOS2 and NOS3, leading to the production of the signaling molecule nitric oxide (NO) by endothelial cells. Phosphorylates PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1 and SRC.
Alternative names
CD309, FLK1, VEGFR2, KDR, Vascular endothelial growth factor receptor 2, VEGFR-2, Fetal liver kinase 1, Kinase insert domain receptor, Protein-tyrosine kinase receptor flk-1, FLK-1
What's included?
Recommended products
Ramucirumab ELISA Kit is a Quantitative ELISA kit for the measurement of Ramucirumab in Human in Plasma, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Serum
- Assay type
- Quantitative
- Reactive species
- Human
- Range
- 20 - 600 ng/mL
- Assay Platform
- Microplate
- Sensitivity
- = 20 ng/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 15 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 15 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % | Range 85 - 115 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
This kit is a sandwich based ELISA kit. The color developed is proportional to the amount of Ramucirumab in the sample or standard. Results of samples can be determined directly using the standard curve.
This product is manufactured by BioVision, an Abcam company and was previously called E4698 BioSim™ Ramucirumab (Human) ELISA Kit. E4698-100 is the same size as the 1 x 96 tests size of ab264501.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Ramucirumab is a monoclonal antibody that targets vascular endothelial growth factor receptor 2 (VEGFR-2). It specifically binds to the extracellular domain of VEGFR-2 inhibiting its ability to interact with its natural ligands. VEGFR-2 has a mass of around 150 kDa and is primarily expressed on endothelial cells which line the blood vessels. Ramucirumab acts by blocking angiogenic signals which are critical for new blood vessel formation.
- Biological function summary
VEGFR-2 is an important component in the angiogenesis process which is the formation of new blood vessels from pre-existing ones. It is part of the plasma membrane-spanning receptor tyrosine kinase family. Upon ligand binding VEGFR-2 undergoes dimerization and autophosphorylation activating downstream signaling pathways. These pathways promote endothelial cell proliferation migration and survival. VEGFR-2's role in vessel permeability and inflammation also contributes to its biological significance.
- Pathways
VEGFR-2 is integral within the VEGF signaling pathway and endothelial cell signaling pathways. It closely interacts with vascular endothelial growth factor A (VEGF-A) as its main ligand. This interaction triggers a cascade involving phosphatidylinositol-3-kinase (PI3K) and mitogen-activated protein kinases (MAPK) which are important for vascular development and maintenance. VEGFR-2's activity is balanced by its relation to other receptors like VEGFR-1 ensuring proper vessel formation and function.
- Associated diseases and disorders
Ramucirumab has shown effectiveness for conditions such as gastric cancer and colorectal cancer. These diseases often exhibit abnormal angiogenesis which is important for tumor growth and metastasis. VEGFR-2 is upregulated in these cancers making it a target for therapeutic intervention. Ramucirumab's binding to VEGFR-2 prevents VEGF-mediated tumor vascularization and progression. Its relationship with other proteins such as fibroblast growth factor receptors (FGFRs) in cancer highlights the complexity of angiogenic signaling in malignancies.
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1 product image
Sandwich ELISA - Ramucirumab ELISA Kit (ab264501)
Example of Ramucirumab standard curve.
Typical Standard Curve: This standard curve is for demonstration only. A standard curve must be run with each assay.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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