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AB193733

Rat Activin A ELISA Kit

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(2 Publications)

Rat Activin A ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Rat Activin A in Human, Mouse, Rat in Biofluids, Cell Culture Media samples.

View Alternative Names

Inhibin beta A chain, Activin beta-A chain, Inhba

2 Images
Sandwich ELISA - Rat Activin A ELISA Kit (AB193733)
  • sELISA

Supplier Data

Sandwich ELISA - Rat Activin A ELISA Kit (AB193733)

Typical Standard Curve - Diluent B.

A typical standard curve using ab193733 with Assay Diluent B

Sandwich ELISA - Rat Activin A ELISA Kit (AB193733)
  • sELISA

Supplier Data

Sandwich ELISA - Rat Activin A ELISA Kit (AB193733)

Typical Standard Curve - Diluent A.

A typical standard curve using ab193733 with Assay Diluent A

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse, Rat, Human

Assay type

Sandwich (quantitative)

Sensitivity

< 2 pg/mL

Range

2 - 400 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Rat Activin A ELISA Kit (ab193733) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat Activin A in sera, plasma and cell culture supernatants.

This assay employs an antibody specific for rat Activin A coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures Activin A present in the samples. The wells are washed and biotinylated anti-rat Activin A antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of Activin A bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Cell culture media", "range": "105 - 149 %", "average": "= 121.7" }, { "sample": "Plasma", "range": "127 - 150 %", "average": "= 138.7" }, { "sample": "Serum", "range": "129 - 150 %", "average": "= 143.3" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab193733-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB193733-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB193733-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB193733-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Rat Activin A Detection antibody", "number":"AB193733-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rat Activin A Standard (lyophilized)", "number":"AB193733-CMP02", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB193733-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB193733-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pre-coated rat Activin A Microplate (12 strips x 8 wells)", "number":"AB193733-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB193733-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Activin A also known by its gene name INHBA is a protein involved in various mechanical processes in the human body. This protein forms part of the transforming growth factor-beta (TGF-β) superfamily and has a molecular weight of approximately 25 kDa for its dimeric forms. Activin A is expressed in many tissues including the pituitary gland reproductive organs and placenta. Researchers often use techniques like the Activin A ELISA test kit to measure its concentration in biological samples.
Biological function summary

Activin A plays a significant role in regulating cell differentiation proliferation and apoptosis. As part of a complex Activin A interacts with specific receptors on cell surfaces initiating intracellular signaling cascades that influence these vital processes. It acts as a growth factor in the reproductive system influencing follicle-stimulating hormone (FSH) secretion and is important in embryonic development and wound healing.

Pathways

Activin A participates in pathways such as the TGF-β signaling pathway and the SMAD protein signaling pathway. Within these pathways it interacts with receptor proteins like type I and type II Activin receptors and downstream SMAD proteins. These interactions enable Activin A to regulate gene expression relevant to various cellular functions.

Altered Activin A levels have associations with conditions like cancer and fibrotic diseases. Its overexpression links to some cancer types including colorectal and prostate cancer where it influences tumor growth and metastasis. Moreover its interaction with proteins like myostatin may contribute to muscle wasting disorders. The dysregulation of these pathways highlights the significance of Activin A in pathophysiology.
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Product protocols

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Target data

Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
See full target information Inhba
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroinflammation 20:50 PubMed36829205

2023

TREM2 mediates physical exercise-promoted neural functional recovery in rats with ischemic stroke via microglia-promoted white matter repair.

Applications

Unspecified application

Species

Unspecified reactive species

Jinghui Xu,Liying Zhang,Mingyue Li,Xiaofei He,Jing Luo,Rui Wu,Zhongqiu Hong,Haiqing Zheng,Xiquan Hu

Journal of cellular physiology 235:5649-5665 PubMed31960436

2020

Resistance training-induced muscle hypertrophy is mediated by TGF-β1-Smad signaling pathway in male Wistar rats.

Applications

Unspecified application

Species

Unspecified reactive species

Rohollah Nikooie,Sohil Jafari-Sardoie,Vahid Sheibani,Amir Nejadvaziri Chatroudi
View all publications
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