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AB213919

Rat ADAM12 ELISA Kit

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Rat ADAM12 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Rat ADAM12 in Rat in Cell Culture Media, Biofluids samples.

View Alternative Names

A disintegrin and metalloproteinase domain 12, ADA12_HUMAN, ADAM metallopeptidase domain 12, Disintegrin and metalloproteinase domain-containing protein 12, MCMP, MCMPMltna, MLTN, MLTNA, Meltrin-alpha, Metalloprotease disintegrin 12 transmembrane, OTTHUMP00000046766

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Sandwich ELISA - Rat ADAM12 ELISA Kit (AB213919)
  • sELISA

Supplier Data

Sandwich ELISA - Rat ADAM12 ELISA Kit (AB213919)

Rat ADAM12 ELISA Kit (ab213919) Standard Curve.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Heparin Plasma, Serum

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

< 10 pg/mL

Range

156 - 10000 pg/mL

Assay time

3h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

The Rat ADAM12 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213919) is designed for the quantitative measurement of Rat ADAM12 in cell culture supernatants, serum and plasma (heparin).

The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ADAM12 has been precoated onto 96-well plates. Standards and test samples are added to the wells; a biotinylated detection polyclonal antibody from goat specific for ADAM12 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat ADAM12 amount of sample captured in plate.

ADAM12 (Disintegrin and metalloproteinase domain-containing protein 12), also known as MLTN, is an enzyme that in humans is encoded by the ADAM12 gene. This gene encodes a member of the ADAM (a disintegrin and metalloprotease) protein family. Members of this family are membrane anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This gene has two alternatively spliced transcripts: a shorter secreted form and a longer membrane-bound form. The shorter form is found to stimulate myogenesis. By RT-PCR and immunoblot analyses that expression of mouse Adam12 increases during muscle regeneration, while the levels of other ADAMs remain constant. Immunofluorescence analysis revealed staining of small, newly formed muscle fibers in regenerating but not normal adult muscle cells. By using of fluorescence in situ hybridization, the ADAM12 gene is mapped to human chromosome 10q26.3.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "6261 pg/mL", "standardDeviation": "306.78", "coefficientOfVariability": "= 4.9" }, { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "237 pg/mL", "standardDeviation": "10.9", "coefficientOfVariability": "= 4.6" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "6482 pg/mL", "standardDeviation": "388.92", "coefficientOfVariability": "= 6" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "217 pg/mL", "standardDeviation": "9.54", "coefficientOfVariability": "= 4.4" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "1849 pg/mL", "standardDeviation": "144.22", "coefficientOfVariability": "= 7.8" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "1804 pg/mL", "standardDeviation": "137.1", "coefficientOfVariability": "= 7.6" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ADAM12 also known as meltrin-alpha is a member of the ADAM (a disintegrin and metalloproteinase) family and has a mass of approximately 110 kDa. It functions primarily in the modulation of cell-cell and cell-matrix interactions due to its metalloprotease and disintegrin domains. ADAM12 protein is expressed in various tissues including muscle and placenta. Although found in many cell types it is particularly abundant during embryogenesis and development reflecting its role in these processes.
Biological function summary

The ADAM12 protein holds a role in cell signaling migration and proliferation. It is often part of complexes that interact with other extracellular matrix components influencing cell adhesion and motility. Through its protease activity ADAM12 facilitates growth factor signaling by cleaving membrane-bound precursor proteins to release active growth factors. This activity plays an important role in tissue remodeling and repair.

Pathways

The ADAM12 protein is key in the EGFR signaling pathway where it modulates the availability of epidermal growth factor (EGF) by shedding EGF-like ligands from the cell surface. This action connects ADAM12 with proteins such as ERBB receptors influencing cell division and growth. Another significant pathway is the TGF-beta signaling pathway where ADAM12's interaction with latent TGF-beta complexes can impact the bioavailability of this cytokine therefore relating to proliferation and differentiation of cells.

ADAM12 is linked to cancer progression and metastasis as its activity can positively influence tumor cell invasiveness and growth. Elevated levels of ADAM12 are observed in certain cancer types including breast and prostate cancer. Additionally it associates with metabolic disorders such as obesity where ADAM12 impacts adipogenesis and insulin sensitivity. In cancer ADAM12 interacts with proteins like matrix metalloproteinases (MMPs) to remodel the tumor microenvironment promoting cancer cell invasion.
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Product protocols

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Target data

See full target information Adam12
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websiteProtocolBooklet
en
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