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AB108784

Rat Adiponectin ELISA Kit

5

(1 Review)

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(13 Publications )

Rat Adiponectin ELISA Kit is a Sandwich ELISA for the measurement of Rat Adiponectin in Rat in Biofluids, Cell Culture Media samples.
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Sandwich ELISA - Rat Adiponectin ELISA Kit (AB108784)
  • sELISA

Supplier Data

Sandwich ELISA - Rat Adiponectin ELISA Kit (AB108784)

Representative Standard Curve using ab108784.

Sandwich ELISA - Rat Adiponectin ELISA Kit (AB108784)
  • sELISA

Lab

Sandwich ELISA - Rat Adiponectin ELISA Kit (AB108784)

Adiponectin measured in tissue lysates showing quantity (ng) per 1 million cells

Sandwich ELISA - Rat Adiponectin ELISA Kit (AB108784)
  • sELISA

Lab

Sandwich ELISA - Rat Adiponectin ELISA Kit (AB108784)

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Cell culture supernatant, Serum

Reacts with

Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.31 ng/mL

Range

1.563 - 100 ng/mL

Assay time

4h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Adiponectin Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of rat Adiponectin in urine, plasma, serum, and cell culture supernatants.

An Adiponectin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Adiponectin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Adiponectin captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.8" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.3" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108784-1x96Tests", "assetComponentDetails": [ { "size":"1 x 2 mL", "name":"1X Standard Diluent", "number":"AB108784-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Adiponectin Microplate (12 x 8 well strips)", "number":"AB108784-CMP03", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB108784-CMP05", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108784-CMP06", "productcode":"" }, { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108784-CMP07", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108784-CMP08", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108784-CMP09", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108784-CMP10", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Rat Adiponectin Antibody", "number":"AB108784-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Adiponectin Standard", "number":"AB108784-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Adiponectin also known as ADIPOQ or adipocyte complement-related protein of 30 kDa (Acrp30) is a 30 kDa protein that plays a significant mechanical role in energy homeostasis. Adiponectin is secreted predominantly by adipose tissue and circulates in the blood. This protein forms various multimeric structures including low-molecular-weight medium-molecular-weight and high-molecular-weight forms each with distinct biological functions. Its expression in adipose tissue links it closely to metabolic processes and functions related to fat storage and energy balance.
Biological function summary

Adiponectin influences glucose regulation and fatty acid oxidation. It acts as a hormone with several metabolic roles including anti-diabetic anti-atherogenic and anti-inflammatory properties. Adiponectin participates in forming a complex with other proteins such as AdipoR1 and AdipoR2 which are receptors facilitating signal transduction. The interaction of adiponectin with its receptors leads to the induction of several lipid and glucose metabolism pathways important for maintaining cellular energy balance.

Pathways

Many regulatory cascades are influenced by adiponectin. This protein is integral to the AMPK signaling pathway and the PPAR signaling pathway. In the AMPK pathway adiponectin enhances insulin sensitivity and stimulates oxidation of fatty acids in muscle tissue. Through the PPAR pathway it influences lipid metabolism and storage. Adiponectin interacts with key proteins such as leptin and resistin coordinating various metabolic pathways to balance energy and glucose levels making it a critical factor in metabolic regulation.

Reduced levels of adiponectin associate with conditions like type 2 diabetes and cardiovascular disease. Lower circulating adiponectin concentrations correlate with obesity leading to increased insulin resistance and higher risk of type 2 diabetes. In cardiovascular disease this protein's role links to its anti-inflammatory properties and ability to decrease the proliferation of vascular smooth muscle cells. Adiponectin also interacts with proteins like cytokines that play a role in these conditions affecting the body’s inflammatory responses and metabolic processes highlighting its importance in health and disease management.
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Product protocols

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Target data

See full target information Adipoq

Alternative Names

30 kDa adipocyte complement-related protein, ACDC, ADIPO_HUMAN, ADIPQTL1, ADPN, Acrp 30, Adipocyte, Adipocyte C1q and collagen domain containing protein, Adipocyte complement related protein of 30 kDa, Adipocyte complement-related 30 kDa protein, Adiponectin, Adiponectin precursor, Adipose most abundant gene transcript 1, Adipose most abundant gene transcript 1 protein, Adipose specific collagen like factor, C1q and collagen domain-containing protein, GBP 28, Gelatin-binding protein, OTTHUMP00000210047, adipocyte-specific secretory protein, adiponectin, C1Q and collagen domain containing, apM-1, gelatin-binding protein 28
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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Journal of physiology and biochemistry 81:137-156 PubMed39576482

2024

Modification of adipogenesis and oxidative stress by quercetin: positive or negative impact on adipose tissue metabolism of obese diabetic Zucker rats?

Applications

Unspecified application

Species

Unspecified reactive species

Katarína Kršková,Viktória Dobrócsyová,Kristína Ferenczyová,Jana Hricovíniová,Barbora Kaločayová,Ulrika Duľová,Mahdi Bozorgnia,Monika Barteková,Štefan Zorad

Frontiers in nutrition 9:1007679 PubMed36313074

2022

extract and chlorogenic acid attenuates high-fat-diet- induced prediabetes CTRPs-AdipoRs-AMPK/PPARα axes.

Applications

Unspecified application

Species

Unspecified reactive species

Chengcheng Guo,Xiaoyuan Zhang,Yingxiang Yu,Yifan Wu,Lan Xie,Cuiqing Chang

Oxidative medicine and cellular longevity 2022:5265616 PubMed36035217

2022

Contribution of Adiponectin/Carnitine Palmityl Transferase 1A-Mediated Fatty Acid Metabolism during the Development of Idiopathic Pulmonary Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjuan Wu,Guojun Zhang,Lingxiao Qiu,Xueya Liu,Shuai Zhou,Jizhen Wu

Frontiers in cell and developmental biology 9:762335 PubMed34790669

2021

Paracrine Effects of Recombinant Human Adiponectin Promote Bone Regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Yanping Gong,Yang Wang,Yiqing Zhang,Liangchen Wang,Lijuan Wan,Yuan Zu,Chunlin Li,Xin Wang,Zhong-Kai Cui

Molecules (Basel, Switzerland) 24: PubMed31623151

2019

Modulation of Adipogenesis and Oxidative Status by Quercetin and Ochratoxin A: Positive or Negative Impact on Rat Adipocyte Metabolism?

Applications

Unspecified application

Species

Unspecified reactive species

Viktoria Dobrocsyova,Katarina Krskova,Marcela Capcarova,Stefan Zorad

Placenta 78:44-53 PubMed30955710

2019

Maternal high-fat diet sex-specifically alters placental morphology and transcriptome in rats: Assessment by next-generation sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Ju Lin,Li-Tung Huang,Ching-Chou Tsai,Jiunn-Ming Sheen,Mao-Meng Tiao,Hong-Ren Yu,I-Chun Lin,You-Lin Tain

Journal of obesity 2018:5341384 PubMed30510798

2018

(Yacon) Flour Improves Visceral Adiposity and Metabolic Parameters in High-Fat-Diet-Fed Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Stella Maris Honoré,Maria Virginia Grande,Jorge Gomez Rojas,Sara Serafina Sánchez

Molecular medicine reports 16:5580-5586 PubMed28849192

2017

Chinese herb extract improves liver steatosis by promoting the expression of high molecular weight adiponectin in NAFLD rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xudong Liu,Wangxia Tong,Xiaofang Zhao,Hongxing Zhang,Yanfang Tang,Xin Deng

Frontiers in behavioral neuroscience 11:113 PubMed28706476

2017

Long-Term Sex-Dependent Vulnerability to Metabolic challenges in Prenatally Stressed Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Pamela Panetta,Alessandra Berry,Veronica Bellisario,Sara Capoccia,Carla Raggi,Alessia Luoni,Linda Longo,Marco A Riva,Francesca Cirulli

The Journal of physiology 593:4067-79 PubMed26108677

2015

Globular adiponectin ameliorates metabolic insulin resistance via AMPK-mediated restoration of microvascular insulin responses.

Applications

Unspecified application

Species

Unspecified reactive species

Lina Zhao,Zhuo Fu,Jing Wu,Kevin W Aylor,Eugene J Barrett,Wenhong Cao,Zhenqi Liu
View all publications
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