Rat Adiponectin ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Rat Adiponectin in Rat in Urine, Plasma, Cell culture supernatant, Serum samples.
Images
Publications
- Frontiers in nutrition 9:10076792022extract and chlorogenic acid attenuates high-fat-diet- induced prediabetes CTRPs-AdipoRs-AMPK/PPARα axes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChengcheng Guo,Xiaoyuan Zhang,Yingxiang Yu,Yifan Wu,Lan Xie,Cuiqing ChangPubMed 36313074
- Oxidative medicine and cellular longevity 2022:52656162022Contribution of Adiponectin/Carnitine Palmityl Transferase 1A-Mediated Fatty Acid Metabolism during the Development of Idiopathic Pulmonary Fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenjuan Wu,Guojun Zhang,Lingxiao Qiu,Xueya Liu,Shuai Zhou,Jizhen WuPubMed 36035217
Key facts
- Detection method
Colorimetric
- Sample types
Urine, Plasma, Cell culture supernatant, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Rat
- Range
6.25 - 50 ng/mL
- Assay time
4h
- Sensitivity
= 0.31 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Product
Target data
Alternative names
30 kDa adipocyte complement-related protein, ACDC, ADIPO_HUMAN, ADIPQTL1, ADPN, Acrp 30, Adipocyte, Adipocyte C1q and collagen domain containing protein, Adipocyte complement related protein of 30 kDa, Adipocyte complement-related 30 kDa protein, Adiponectin, Adiponectin precursor, Adipose most abundant gene transcript 1, Adipose most abundant gene transcript 1 protein, Adipose specific collagen like factor, C1q and collagen domain-containing protein, GBP 28, Gelatin-binding protein, OTTHUMP00000210047, adipocyte-specific secretory protein, adiponectin, C1Q and collagen domain containing, apM-1, gelatin-binding protein 28
What's included?
Recommended products
Rat Adiponectin ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Rat Adiponectin in Rat in Urine, Plasma, Cell culture supernatant, Serum samples.
Alternative names
30 kDa adipocyte complement-related protein, ACDC, ADIPO_HUMAN, ADIPQTL1, ADPN, Acrp 30, Adipocyte, Adipocyte C1q and collagen domain containing protein, Adipocyte complement related protein of 30 kDa, Adipocyte complement-related 30 kDa protein, Adiponectin, Adiponectin precursor, Adipose most abundant gene transcript 1, Adipose most abundant gene transcript 1 protein, Adipose specific collagen like factor, C1q and collagen domain-containing protein, GBP 28, Gelatin-binding protein, OTTHUMP00000210047, adipocyte-specific secretory protein, adiponectin, C1Q and collagen domain containing, apM-1, gelatin-binding protein 28
Key facts
- Detection method
Colorimetric
- Sample types
Urine, Plasma, Cell culture supernatant, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Rat
- Range
6.25 - 50 ng/mL
- Assay time
4h
- Assay Platform
Microplate
- Sensitivity
= 0.31 ng/mL
- General recovery
98%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. 4.8 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. 10.3 |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
Multi
- Storage information
Please refer to protocols
Notes
Abcam's Adiponectin Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of rat Adiponectin in urine, plasma, serum, and cell culture supernatants.
An Adiponectin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Adiponectin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Adiponectin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Supplementary info
- Activity summary
Adiponectin also known as ADIPOQ or adipocyte complement-related protein of 30 kDa (Acrp30) is a 30 kDa protein that plays a significant mechanical role in energy homeostasis. Adiponectin is secreted predominantly by adipose tissue and circulates in the blood. This protein forms various multimeric structures including low-molecular-weight medium-molecular-weight and high-molecular-weight forms each with distinct biological functions. Its expression in adipose tissue links it closely to metabolic processes and functions related to fat storage and energy balance.
- Biological function summary
Adiponectin influences glucose regulation and fatty acid oxidation. It acts as a hormone with several metabolic roles including anti-diabetic anti-atherogenic and anti-inflammatory properties. Adiponectin participates in forming a complex with other proteins such as AdipoR1 and AdipoR2 which are receptors facilitating signal transduction. The interaction of adiponectin with its receptors leads to the induction of several lipid and glucose metabolism pathways important for maintaining cellular energy balance.
- Pathways
Many regulatory cascades are influenced by adiponectin. This protein is integral to the AMPK signaling pathway and the PPAR signaling pathway. In the AMPK pathway adiponectin enhances insulin sensitivity and stimulates oxidation of fatty acids in muscle tissue. Through the PPAR pathway it influences lipid metabolism and storage. Adiponectin interacts with key proteins such as leptin and resistin coordinating various metabolic pathways to balance energy and glucose levels making it a critical factor in metabolic regulation.
- Associated diseases and disorders
Reduced levels of adiponectin associate with conditions like type 2 diabetes and cardiovascular disease. Lower circulating adiponectin concentrations correlate with obesity leading to increased insulin resistance and higher risk of type 2 diabetes. In cardiovascular disease this protein's role links to its anti-inflammatory properties and ability to decrease the proliferation of vascular smooth muscle cells. Adiponectin also interacts with proteins like cytokines that play a role in these conditions affecting the body’s inflammatory responses and metabolic processes highlighting its importance in health and disease management.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Sandwich ELISA - Rat Adiponectin ELISA Kit (ab108784)
Adiponectin measured in tissue lysates showing quantity (ng) per 1 million cells
Sandwich ELISA - Rat Adiponectin ELISA Kit (ab108784)
Sandwich ELISA - Rat Adiponectin ELISA Kit (ab108784)
Representative Standard Curve using ab108784.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com