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AB239421

Rat Adiponectin ELISA Kit

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(10 Publications)

Rat Adiponectin ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat Adiponectin with a sensitivity of 3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 10 citations
4 Images
Sandwich ELISA - Rat Adiponectin ELISA Kit (AB239421)
  • sELISA

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Sandwich ELISA - Rat Adiponectin ELISA Kit (AB239421)

Example of rat Adiponectin standard curve in Sample Diluent NS. The Adiponectin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Rat Adiponectin ELISA Kit (AB239421)
  • sELISA

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Sandwich ELISA - Rat Adiponectin ELISA Kit (AB239421)

Interpolated concentrations of native Adiponectin in rat serum, and plasma samples.

The concentrations of Adiponectin were measured in duplicates, interpolated from the Adiponectin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 1.7x104, plasma (citrate) 1 : 1.7x104, plasma (EDTA) 1 : 1.7x104, and plasma (heparin) 1 : 1.7x104. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Adiponectin concentration was determined to be 4444 ng/mL in serum, 4789 ng/mL in plasma (citrate), 3725 ng/mL in plasma (EDTA) and 5091 ng/mL in plasma (heparin).

Sandwich ELISA - Rat Adiponectin ELISA Kit (AB239421)
  • sELISA

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Sandwich ELISA - Rat Adiponectin ELISA Kit (AB239421)

Interpolated concentrations of native Adiponectin in rat urine and cell culture supernatant samples. The concentrations of Adiponectin were measured in duplicates, interpolated from the Adiponectin standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 25%, and lung cell culture supernatant 1 : 67. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Adiponectin concentration was determined to be 2,147 pg/mL in urine and 21,756 pg/mL in lung cell culture supernatant.

Sandwich ELISA - Rat Adiponectin ELISA Kit (AB239421)
  • sELISA

Unknown

Sandwich ELISA - Rat Adiponectin ELISA Kit (AB239421)

Rat Adiponectin Standard Curve Comparison. Standard Curve comparison between rat Adiponectin SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Key facts

Detection method

Colorimetric

Sample types

Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 3 pg/mL

Range

9.38 - 600 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Rat Adiponectin ELISA Kit (ab239421) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Adiponectin protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat Adiponectin with 3 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.3" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "89 - 95 %", "average": "= 92" }, { "sample": "Serum", "range": "93 - 114 %", "average": "= 101" }, { "sample": "EDTA Plasma", "range": "89 - 96 %", "average": "= 91" }, { "sample": "Urine", "range": "63 - 87 %", "average": "= 78" }, { "sample": "Heparin Plasma", "range": "89 - 91 %", "average": "= 89" }, { "sample": "Citrate plasma", "range": "85 - 94 %", "average": "= 91" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab239421-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB239421-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB239421-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB239421-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB239421-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB239421-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat Adiponectin Capture Antibody", "number":"AB239421-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rat Adiponectin Lyophilized Recombinant Protein", "number":"AB239421-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB239421-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat Adiponectin Detector Antibody", "number":"AB239421-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB239421-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Adiponectin also known as ADIPOQ or adipocyte complement-related protein of 30 kDa (Acrp30) is a 30 kDa protein that plays a significant mechanical role in energy homeostasis. Adiponectin is secreted predominantly by adipose tissue and circulates in the blood. This protein forms various multimeric structures including low-molecular-weight medium-molecular-weight and high-molecular-weight forms each with distinct biological functions. Its expression in adipose tissue links it closely to metabolic processes and functions related to fat storage and energy balance.
Biological function summary

Adiponectin influences glucose regulation and fatty acid oxidation. It acts as a hormone with several metabolic roles including anti-diabetic anti-atherogenic and anti-inflammatory properties. Adiponectin participates in forming a complex with other proteins such as AdipoR1 and AdipoR2 which are receptors facilitating signal transduction. The interaction of adiponectin with its receptors leads to the induction of several lipid and glucose metabolism pathways important for maintaining cellular energy balance.

Pathways

Many regulatory cascades are influenced by adiponectin. This protein is integral to the AMPK signaling pathway and the PPAR signaling pathway. In the AMPK pathway adiponectin enhances insulin sensitivity and stimulates oxidation of fatty acids in muscle tissue. Through the PPAR pathway it influences lipid metabolism and storage. Adiponectin interacts with key proteins such as leptin and resistin coordinating various metabolic pathways to balance energy and glucose levels making it a critical factor in metabolic regulation.

Reduced levels of adiponectin associate with conditions like type 2 diabetes and cardiovascular disease. Lower circulating adiponectin concentrations correlate with obesity leading to increased insulin resistance and higher risk of type 2 diabetes. In cardiovascular disease this protein's role links to its anti-inflammatory properties and ability to decrease the proliferation of vascular smooth muscle cells. Adiponectin also interacts with proteins like cytokines that play a role in these conditions affecting the body’s inflammatory responses and metabolic processes highlighting its importance in health and disease management.
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Product protocols

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Target data

See full target information ADIPOQ
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Life (Basel, Switzerland) 14: PubMed39202687

2024

Morin Prevents Non-Alcoholic Hepatic Steatosis in Obese Rats by Targeting the Peroxisome Proliferator-Activated Receptor Alpha (PPARα).

Applications

Unspecified application

Species

Unspecified reactive species

Laila Naif Al-Harbi

Nutrition research and practice 18:180-193 PubMed38584817

2024

Effects of root extract on adipogenesis and lipogenesis in 3T3-L1 adipocytes and SD rat models.

Applications

Unspecified application

Species

Unspecified reactive species

Kyoung Kon Kim,Hye Rim Lee,Sun Min Jang,Tae Woo Kim

Nutrients 15: PubMed38004149

2023

Esculeogenin A, a Glycan from Tomato, Alleviates Nonalcoholic Fatty Liver Disease in Rats through Hypolipidemic, Antioxidant, and Anti-Inflammatory Effects.

Applications

Unspecified application

Species

Unspecified reactive species

Jwharah M Al Jadani,Nawal A Albadr,Ghedeir M Alshammari,Soheir A Almasri,Farah Fayez Alfayez,Mohammed Abdo Yahya

Pharmaceutics 15: PubMed38004588

2023

Carob Extract ( L.): Effects on Dyslipidemia and Obesity in a High-Fat Diet-Fed Rat Model.

Applications

Unspecified application

Species

Unspecified reactive species

Aleksandar Rašković,Nikola Martić,Ana Tomas,Bojana Andrejić-Višnjić,Milana Bosanac,Marko Atanasković,Marko Nemet,Radmila Popović,Marko Krstić,Saša Vukmirović,Nebojša Stilinović

Nutrients 15: PubMed36986201

2023

Activation of AMPK Entails the Protective Effect of Royal Jelly against High-Fat-Diet-Induced Hyperglycemia, Hyperlipidemia, and Non-Alcoholic Fatty Liver Disease in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Alaa Hasanain Felemban,Ghedeir M Alshammari,Abu ElGasim Ahmed Yagoub,Laila Naif Al-Harbi,Maha H Alhussain,Mohammed Abdo Yahya

Nutrients 15: PubMed36615764

2022

The Synergistic Protective Effect of γ-Oryzanol (OZ) and N-Acetylcysteine (NAC) against Experimentally Induced NAFLD in Rats Entails Hypoglycemic, Antioxidant, and PPARα Stimulatory Effects.

Applications

Unspecified application

Species

Unspecified reactive species

Ashwag H Alwadani,Soheir A Almasri,Amal A Aloud,Nawal A Albadr,Ghedeir M Alshammari,Mohammed Abdo Yahya

International journal of environmental research and public health 19: PubMed36360622

2022

Vitamin D and Swimming Exercise Prevent Obesity in Rats under a High-Fat Diet via Targeting FATP4 and TLR4 in the Liver and Adipose Tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Eman Kolieb,Shymaa Ahmed Maher,Mohammed Nader Shalaby,Amnah Mohammed Alsuhaibani,Afaf Alharthi,Wael A Hassan,Karima El-Sayed

Nutrients 14: PubMed35565759

2022

Anti-Hyperlipidemia, Hypoglycemic, and Hepatoprotective Impacts of Pearl Millet ( L.) Grains and Their Ethanol Extract on Rats Fed a High-Fat Diet.

Applications

Unspecified application

Species

Unspecified reactive species

Nadiah S Alzahrani,Ghedeir M Alshammari,Afaf El-Ansary,Abu ElGasim A Yagoub,Musarat Amina,Ali Saleh,Mohammed Abdo Yahya

International journal of molecular sciences 22: PubMed34638520

2021

Detection and Evaluation of Serological Biomarkers to Predict Osteoarthritis in Anterior Cruciate Ligament Transection Combined Medial Meniscectomy Rat Model.

Applications

Unspecified application

Species

Unspecified reactive species

Nian-Cih Huang,Tsorng-Shyang Yang,Prabhakar Busa,Ching-Ling Lin,Ya-Chieh Fang,Ing-Jung Chen,Chih-Shung Wong

Saudi journal of biological sciences 28:3333-3342 PubMed34121870

2021

leaf extracts produce anti-obesity, hypoglycemic, anti-hyperlipidemic, and hepatoprotective effects on high-fat diet fed rats.

Applications

Unspecified application

Species

Unspecified reactive species

Dalal A Alkhudhayri,Magdi A Osman,Ghedeir M Alshammari,Salah A Al Maiman,Mohammed Abdo Yahya
View all publications
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