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AB288178

Rat AGT ELISA Kit

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Rat AGT ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat AGT with a sensitivity of 4.73 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Serpina8, Agt, Angiotensinogen, Serpin A8

3 Images
ELISA - Rat AGT ELISA Kit (AB288178)
  • ELISA

Supplier Data

ELISA - Rat AGT ELISA Kit (AB288178)
Sandwich ELISA - Rat AGT ELISA Kit (AB288178)
  • sELISA

Supplier Data

Sandwich ELISA - Rat AGT ELISA Kit (AB288178)

Example of rat AGT standard curve in Sample Diluent NS. Example rat AGT standard curve. Background-subtracted data values (mean =/- SD) are graphed.

Sandwich ELISA - Rat AGT ELISA Kit (AB288178)
  • sELISA

Supplier Data

Sandwich ELISA - Rat AGT ELISA Kit (AB288178)

Interpolated concentrations of rat AGT in rat serum and plasma samples. The endogenous concentration of AGT was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 50,000 serum, plasma (citrate), plasma (heparin) and plasma (EDTA). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 4.73 pg/mL

Range

14.063 - 900 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Rat AGT ELISA Kit ab288178 is a rapid single-wash 90-min Sandwich ELISA to measure Rat AGT in citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 4.73 pg/mL.

How the assay works

Rat AGT SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Rat AGT ELISA Kit ab288178 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Angiotensinogen antibody [EPR24418-99] - BSA and Azide free (Capture) ab281031
- Anti-Angiotensinogen antibody [EPR24418-38] - BSA and Azide free (Detector) ab281031
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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.5" } ]
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Recovery

[ { "sample": "Serum", "range": "97 - 120 %", "average": "= 109" }, { "sample": "EDTA Plasma", "range": "97 - 102 %", "average": "= 99" }, { "sample": "Heparin Plasma", "range": "89 - 96 %", "average": "= 93" }, { "sample": "Citrate plasma", "range": "95 - 118 %", "average": "= 105" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab288178-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB288178-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB288178-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"Rat AGT Lyophilized Recombinant Protein", "number":"AB288178-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB288178-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat AGT Capture Antibody", "number":"AB288178-CMP07", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB288178-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB288178-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB288178-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"10X Rat AGT Detector Antibody", "number":"AB288178-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB288178-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Angiotensin is a peptide hormone critical in regulating blood pressure and fluid balance. Often referred to as Angiotensin I II or III depending on its form this target has a variable mass depending on the specific peptide variant. It primarily expresses in the liver where it is originally synthesized as a precursor protein called angiotensinogen. This protein circulates in the bloodstream and is cleaved to form active peptides when needed. Angiotensin acts on vascular smooth muscles and adrenal glands playing a significant role in vasoconstriction and aldosterone secretion.
Biological function summary

The peptide interacts with specific receptors in different tissues to exert its effects. It is part of the renin-angiotensin system (RAS) where it forms a functional complex with enzymes such as renin and angiotensin-converting enzyme (ACE). This system includes various feedback mechanisms maintaining homeostasis within the cardiovascular and renal systems. Angiotensin II the most active form binds to receptors on blood vessels increasing their constriction and therefore raising blood pressure. It also influences sodium and water retention through its action on the kidneys.

Pathways

This peptide fits into the larger framework of the RAS which is important for the regulation of vascular resistance. Angiotensin interacts directly with angiotensinogen which renin processes to form Angiotensin I. Subsequently ACE converts Angiotensin I to Angiotensin II. This cascade affects several downstream pathways influencing cardiovascular function and fluid retention. By impacting other proteins involved in these pathways such as aldosterone it plays an integral role in circulatory and renal physiology.

Angiotensin is closely linked to hypertension and heart failure. Dysregulation of the renin-angiotensin system or overproduction of Angiotensin II often leads to elevated blood pressure contributing to these conditions. Angiotensin receptor blockers (ARBs) and ACE inhibitors serve as therapeutic interventions in these diseases. Moreover in the case of heart failure excessive activity of Angiotensin can worsen the condition by increasing cardiac workload. It interacts with other proteins like ACE in these disorders forming a target for therapeutic modulation to improve patient outcomes.
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Product protocols

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Target data

Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis.. Angiotensin-2. Acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone. Acts by binding to angiotensin receptors AGTR1 and AGTR2 (By similarity). Also binds the DEAR/FBXW7-AS1 receptor (PubMed : 9508787).. Angiotensin-3. Stimulates aldosterone release.. Angiotensin 1-7. Is a ligand for the G-protein coupled receptor MAS1 (By similarity). Has vasodilator and antidiuretic effects (By similarity). Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets (PubMed : 18026570).
See full target information Agt
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websiteProtocolBooklet
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