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AB285264

Rat Alanine Aminotransferase (ALT) ELISA Kit

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(3 Publications)

Rat Alanine Aminotransferase (ALT) ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Rat Alanine Aminotransferase (ALT) in Rat in Biofluids, Cell/Tissue Extracts samples.

View Alternative Names

Aat1, Gpt1, Gpt, Alanine aminotransferase 1, ALT1, Glutamate pyruvate transaminase 1, Glutamic--alanine transaminase 1, Glutamic--pyruvic transaminase 1, GPT 1

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Sandwich ELISA - Rat Alanine Aminotransferase (ALT) ELISA Kit (AB285264)
  • sELISA

Supplier Data

Sandwich ELISA - Rat Alanine Aminotransferase (ALT) ELISA Kit (AB285264)

Rat Alanine Aminotransferase (ALT) ELISA Kit.

Typical Standard Curve : These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Tissue Homogenate, Serum, Other biological fluids

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

< 0.469 ng/mL

Range

0.78 - 50 ng/mL

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Alanine aminotransferase 1 mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins through calcium-binding. Among the enzymes to be stimulated by the calmodulin-calcium complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.

This GPT ELISA kit (ab285264, E4325) is a sandwich ELISA assay for the quantitative measurement of rat GPT in serum, plasma and cell culture supernatants. The density of color is proportional to the amount of rat GPT captured from the samples

This product is manufactured by BioVision, an Abcam company and was previously called E4325 Alanine Aminotransferase 1 (GPT) (rat) ELISA Kit. E4325-100 is the same size as the 96 test size of ab285264.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 8" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab285264-96Test", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"25X Wash buffer", "number":"AB285264-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Substrate", "number":"AB285264-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"Antibody dilution buffer", "number":"AB285264-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"Stop Solution", "number":"AB285264-CMP11", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample and Standard Dilution Buffer", "number":"AB285264-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"SABC dilution buffer", "number":"AB285264-CMP05", "productcode":"" }, { "size":"1 x 120 µL", "name":"HRP-Streptavidin Conjugate (SABC)", "number":"AB285264-CMP08", "productcode":"" }, { "size":"5 x 1 Unit", "name":"Plate sealers", "number":"AB285264-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Standard", "number":"AB285264-CMP03", "productcode":"" }, { "size":"1 x 120 µL", "name":"Biotin/Detection antibody (Concentrated)", "number":"AB285264-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Micro ELISA Plate", "number":"AB285264-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alanine transaminase also known as ALT or alanine aminotransferase is an important enzyme in the body. It has a molecular mass of approximately 54 kDa and catalyzes the conversion between alanine and pyruvate by transferring an amino group. ALT is mainly expressed in the liver but also present in other tissues like the heart and muscles. It is often measured as 'ALT activity' in clinical settings to ascertain liver health. In laboratories an 'ALT assay' or 'ALT kit' can determine alanine transaminase activity.
Biological function summary

Alanine transaminase plays a critical role in amino acid metabolism and energy production. It facilitates the transamination process which is significant for the balance of amino acids and carbohydrates. ALT as a standalone enzyme does not form part of a protein complex but works closely with alanine aminotransferase reactions. It helps maintain the cellular nitrogen balance linking nitrogen metabolism with energy pathways.

Pathways

The role of alanine transaminase is to interconnect carbohydrate and amino acid metabolism. It participates in the gluconeogenesis pathway and the citric acid cycle important for energy production. Through its activity in these pathways it interacts with proteins like aspartate aminotransferase (AST) affecting the balance of amino and keto acids in cells.

Alanine transaminase levels indicate liver conditions such as hepatitis and liver cirrhosis. Elevated ALT activity suggests liver damage or inflammation. It also connects to conditions involving muscular injury. In these scenarios increased presence and activity of AST often accompany ALT providing a broader picture of the extent of tissue damage. Understanding the involvement of ALT in these pathways helps diagnose and monitor the progression of relevant health issues.
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Product protocols

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Target data

Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles (By similarity).
See full target information Gpt
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nutrients 16: PubMed38931176

2024

The Metabolic and Endocrine Effects of a 12-Week Allulose-Rich Diet.

Applications

Unspecified application

Species

Unspecified reactive species

Kevin B Cayabyab,Marley J Shin,Micah S Heimuli,Iris J Kim,Dominic P D'Agostino,Richard J Johnson,Andrew P Koutnik,Nick Bellissimo,David M Diamond,Nicholas G Norwitz,Juan A Arroyo,Paul R Reynolds,Benjamin T Bikman

Cells 13: PubMed38534382

2024

Tofogliflozin Delays Portal Hypertension and Hepatic Fibrosis by Inhibiting Sinusoidal Capillarization in Cirrhotic Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Shohei Asada,Kosuke Kaji,Norihisa Nishimura,Aritoshi Koizumi,Takuya Matsuda,Misako Tanaka,Nobuyuki Yorioka,Shinya Sato,Koh Kitagawa,Tadashi Namisaki,Takemi Akahane,Hitoshi Yoshiji

International journal of molecular medicine 50: PubMed35686541

2022

Rifaximin enhances the L‑carnitine‑mediated preventive effects on skeletal muscle atrophy in cirrhotic rats by modulating the gut‑liver‑muscle axis.

Applications

Unspecified application

Species

Unspecified reactive species

Koji Murata,Kosuke Kaji,Norihisa Nishimura,Masahide Enomoto,Yuki Fujimoto,Soichi Takeda,Yuki Tsuji,Yukihisa Fujinaga,Hiroaki Takaya,Hideto Kawaratani,Tadashi Namisaki,Takemi Akahane,Hitoshi Yoshiji
View all publications
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