AB108789

Rat Albumin ELISA Kit

Name: Rat Albumin ELISA Kit (ab108789) | Abcam
Brand: Abcam Limited
SKU: AB108789

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(32 Publications)

Rat Albumin ELISA Kit is a sandwich ELISA designed to quantify Rat Albumin with a sensitivity of 0.39 ng/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 0.781 - 200 ng/mL
- Cited in over 20 publications

View Alternative Names

Albumin, Alb

2 Images

Sandwich ELISA - Rat Albumin ELISA Kit (AB108789)

sELISA

Supplier Data

Sandwich ELISA - Rat Albumin ELISA Kit (AB108789)

Representative Standard Curve using ab108789.

sELISA

Lab

Sandwich ELISA - Rat Albumin ELISA Kit (AB108789)

Rat albumin measured in tissue lysates showing quantity (microgram) per mg protein

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum, Urine

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 0.39 ng/mL

Range

0.781 - 200 ng/mL

Assay time

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Rat Albumin ELISA Kit ab108789 is a sandwich ELISA to measure Rat Albumin in serum, plasma, urine with a sensitivity of 0.39 ng/ml.

How the assay works

An Albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Albumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Albumin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Rat Albumin ELISA Kit ab108789 protocol summary

1. Add standard or sample to appropriate wells. Incubate at room temperature
2. Wash and add prepared biotin antibody to each well. Incubate at room temperature.
3. Wash and add prepared Streptavidin-Peroxidase Conjugate. Incubate at room temperature
4. Add Chromogen Substrate to each well. Incubate at room temperature
5. Add Stop Solution to each well. Read at 450 nm immediately.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.6" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.4" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108789-1x96Tests", "assetComponentDetails": [ { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108789-CMP04", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108789-CMP09", "productcode":"" }, { "size":"1 x 150 µL", "name":"40X Biotinylated Rat Albumin Antibody", "number":"AB108789-CMP02", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108789-CMP08", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108789-CMP07", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108789-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Albumin Standard", "number":"AB108789-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Albumin Microplate (12 x 8 well strips)", "number":"AB108789-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB108789-CMP01", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Albumin also known as ALB is a major plasma protein with a molecular mass of approximately 66.5 kDa. It predominantly gets synthesized in the liver and found abundantly in blood plasma. Albumin serves multiple functions including maintaining osmotic pressure and acting as a carrier protein for various endogenous substances like hormones fatty acids and bilirubin.

Biological function summary

Albumin plays a critical role in transporting small molecules like metabolites and drugs through the circulatory system. It is not a part of any complex but functions independently to bind transport and release various ligands. Albumin's affinity for hydrophobic molecules makes it essential for they to be solubilized in blood for proper metabolism and excretion.

Pathways

More than five binding sites on albumin facilitate its role in the fatty acid metabolism and renin-angiotensin system pathways. In fatty acid metabolism albumin interacts with transport proteins carrying fatty acids to tissues for energy production. Additionally during the renin-angiotensin system albumin modulates blood pressure through its interaction with angiotensin II which can influence vasoconstriction.

Researchers link albumin with nephrotic syndrome and liver cirrhosis. In nephrotic syndrome low levels of albumin (albumin low) can cause edema due to imbalanced osmotic pressure. In liver cirrhosis a reduction in albumin synthesis signifies liver damage. Albumin's interaction with proteins like angiotensin II correlates with complications such as hypertension further establishing the protein's role in health and disease contexts.

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Product protocols

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Target data

Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (By similarity). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (By similarity). Does not prevent iron uptake by the bacterial siderophore aerobactin (By similarity).

See full target information Alb

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Publications (32)

Recent publications for all applications. Explore the full list and refine your search

Drug design, development and therapy 19:3925-3947 PubMed40395438

2025

ShenQi ShenKang Granule Alleviates Chronic Kidney Disease by Inhibiting the PI3K/AKT/mTOR Pathway and Restoring Autophagy Flux and Mitochondrial Integrity.

Applications

Unspecified application

Species

Unspecified reactive species

Chenhui Xia,Jiale Zhang,Huixi Chen,Shaofeng Zhou,Weimin Jiang,Huijuan Zheng,Zaoqiang Lin,Qinxiang Tan,Weiwei Sun

Nature communications 16:3953 PubMed40289112

2025

S-nitrosylation of EZH2 alters PRC2 assembly, methyltransferase activity, and EZH2 stability to maintain endothelial homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Ashima Sakhuja,Ritobrata Bhattacharyya,Yash Tushar Katakia,Shyam Kumar Ramakrishnan,Srinjoy Chakraborty,Hariharan Jayakumar,Shailesh Mani Tripathi,Niyati Pandya Thakkar,Sumukh Thakar,Sandeep Sundriyal,Shibasish Chowdhury,Syamantak Majumder

Diagnostics (Basel, Switzerland) 15: PubMed40150124

2025

The Zucker Diabetic Fatty Rat as a Model for Vascular Changes in Diabetic Kidney Disease: Characterising Hydronephrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Amy McDermott,Nathalie Sarup Panduro,Iman Taghavi,Hans Martin Kjer,Stinne Byrholdt Søgaard,Michael Bachmann Nielsen,Jørgen Arendt Jensen,Charlotte Mehlin Sørensen

Bioinformation 20:508-514 PubMed39132239

2024

ameliorates AlCl3 and D-galactose induced nephrotoxicity in rats via modulation of oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Thirupathirao Vishnumukkala,Prarthana Kalerammana Gopalakrishna,Barani Karikalan,Warren Thomas,Saravanan Jagadeesan,Samaila Musa Chiroma,Nurul Huda Mohd Nor,Mohamad Aris Mohd Moklas

Hypertension research : official journal of the Japanese Society of Hypertension 47:1627-1641 PubMed38605139

2024

The soluble (pro)renin receptor promotes a preeclampsia-like phenotype both in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Lachlan G Schofield,Sarah J Delforce,Jennifer C Pryor,Saije K Endacott,Eugenie R Lumbers,Sarah A Marshall,Kirsty G Pringle

AMB Express 13:129 PubMed37985554

2023

Novel grape seed extract nanoparticles attenuate amikacin-induced nephrotoxicity in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Alyaa Farid,Dina Mohamed,Dina Mostafa,Rawan Tarek,Viola Sherif,Gehan Safwat

Diagnostics (Basel, Switzerland) 13: PubMed37892017

2023

Super-Resolution Ultrasound Imaging of Renal Vascular Alterations in Zucker Diabetic Fatty Rats during the Development of Diabetic Kidney Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Stinne Byrholdt Søgaard,Sofie Bech Andersen,Iman Taghavi,Mikkel Schou,Christina Christoffersen,Jens Christian Brings Jacobsen,Hans Martin Kjer,Carsten Gundlach,Amy McDermott,Jørgen Arendt Jensen,Michael Bachmann Nielsen,Charlotte Mehlin Sørensen

Scientific reports 13:16424 PubMed37777583

2023

Melatonin loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles reduce inflammation, inhibit apoptosis and protect rat's liver from the hazardous effects of CCL4.

Applications

Unspecified application

Species

Unspecified reactive species

Alyaa Farid,Valina Michael,Gehan Safwat

Frontiers in endocrinology 14:1219205 PubMed37842294

2023

A comparison of rat models that best mimic immune-driven preeclampsia in humans.

Applications

Unspecified application

Species

Unspecified reactive species

Fahmida Jahan,Goutham Vasam,Yusmaris Cariaco,Abolfazl Nik-Akhtar,Alex Green,Keir J Menzies,Shannon A Bainbridge

EBioMedicine 93:104664 PubMed37331163

2023

H3K27me3-modulated Hofbauer cell BMP2 signalling enhancement compensates for shallow trophoblast invasion in preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Jianye Deng,Hong-Jin Zhao,Ying Zhong,Cuiping Hu,Jinlai Meng,Chunling Wang,Xiangxin Lan,Xiyao Wang,Zi-Jiang Chen,Junhao Yan,Wei Wang,Yan Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Rat Albumin ELISA Kit

Sandwich ELISA - Rat Albumin ELISA Kit (AB108789)

Sandwich ELISA - Rat Albumin ELISA Kit (AB108789)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (32)

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