Rat Albumin ELISA Kit is a Competitive ELISA kit for the measurement of Rat Albumin in Rat in Plasma, Serum samples.
Images
Publications
Filter by
- Clinical science (London, England : 1979) 136:675-6942022Differential effects of cyclo-oxygenase 1 and 2 inhibition on angiogenesis inhibitor-induced hypertension and kidney damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatrina M Mirabito Colafella,Daan C H van Dorst,Rugina I Neuman,Leni van Doorn,Karla Bianca Neves,Augusto C Montezano,Ingrid M Garrelds,Richard van Veghel,René de Vries,Estrellita Uijl,Marian C Clahsen-van Groningen,Hans J Baelde,Anton H van den Meiracker,Rhian M Touyz,Willy Visser,A H Jan Danser,Jorie VersmissenPubMed 35441670
- Brain pathology (Zurich, Switzerland) 31:333-3452021Iron accumulation in the choroid plexus, ependymal cells and CNS parenchyma in a rat strain with low-grade haemolysis of fragile macrocytic red blood cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIsabella Wimmer,Cornelia Scharler,Taro Kadowaki,Sophie Hillebrand,Barbara Scheiber-Mojdehkar,Shuichi Ueda,Monika Bradl,Thomas Berger,Hans Lassmann,Simon HametnerPubMed 33220123
- The Journal of clinical investigation 129:4901-49112019AAV8-vectored suprachoroidal gene transfer produces widespread ocular transgene expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKun Ding et. al.PubMed 31408444
- Frontiers in physiology 8:5172017Change in the Lipid Transport Capacity of the Liver and Blood during Reproduction in Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYufeng Zhang et. al.PubMed 28798692
- Oxidative medicine and cellular longevity 2014:9613262014Urinary excretion of neutrophil gelatinase-associated lipocalin in diabetic rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAbraham Said Arellano-Buendía et. al.PubMed 25243053
Key facts
- Detection method
Colorimetric
- Sample types
Plasma, Serum
- Assay type
Competitive
- Reactive species
Rat
- Range
1.563 - 12.5 µg/mL
- Assay time
2h
- Sensitivity
= 0.74 µg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application cELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (By similarity). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (By similarity). Does not prevent iron uptake by the bacterial siderophore aerobactin (By similarity).
Alternative names
Albumin, Alb
What's included?
Recommended products
Rat Albumin ELISA Kit is a Competitive ELISA kit for the measurement of Rat Albumin in Rat in Plasma, Serum samples.
Alternative names
Albumin, Alb
Key facts
- Detection method
Colorimetric
- Sample types
Plasma, Serum
- Assay type
Competitive
- Reactive species
Rat
- Range
1.563 - 12.5 µg/mL
- Assay time
2h
- Assay Platform
Microplate
- Sensitivity
= 0.74 µg/mL
- General recovery
98%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. 3.8 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. 4.9 |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
Multi
- Storage information
Please refer to protocols
Notes
Abcam's Albumin Rat in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of rat Albumin in plasma and serum.
An Albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Albumin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Albumin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Albumin also known as ALB is a major plasma protein with a molecular mass of approximately 66.5 kDa. It predominantly gets synthesized in the liver and found abundantly in blood plasma. Albumin serves multiple functions including maintaining osmotic pressure and acting as a carrier protein for various endogenous substances like hormones fatty acids and bilirubin.
- Biological function summary
Albumin plays a critical role in transporting small molecules like metabolites and drugs through the circulatory system. It is not a part of any complex but functions independently to bind transport and release various ligands. Albumin's affinity for hydrophobic molecules makes it essential for they to be solubilized in blood for proper metabolism and excretion.
- Pathways
More than five binding sites on albumin facilitate its role in the fatty acid metabolism and renin-angiotensin system pathways. In fatty acid metabolism albumin interacts with transport proteins carrying fatty acids to tissues for energy production. Additionally during the renin-angiotensin system albumin modulates blood pressure through its interaction with angiotensin II which can influence vasoconstriction.
- Associated diseases and disorders
Researchers link albumin with nephrotic syndrome and liver cirrhosis. In nephrotic syndrome low levels of albumin (albumin low) can cause edema due to imbalanced osmotic pressure. In liver cirrhosis a reduction in albumin synthesis signifies liver damage. Albumin's interaction with proteins like angiotensin II correlates with complications such as hypertension further establishing the protein's role in health and disease contexts.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Sandwich ELISA - Rat Albumin ELISA Kit (ab108790)
Rat albumin measured in various samples showing quantity (microgram) per mL of tested sample
Sandwich ELISA - Rat Albumin ELISA Kit (ab108790)
Representative Standard Curve using ab108790.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com