AB108790

Rat Albumin ELISA Kit

Name: Rat Albumin ELISA Kit (ab108790) | Abcam
Brand: Abcam Limited
SKU: AB108790
Price: 780 USD
Availability: InStock

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(6 Publications)

Rat Albumin ELISA Kit is a Competitive ELISA for the measurement of Rat Albumin in Rat in Biofluids samples.

View Alternative Names

Albumin, Alb

2 Images

Sandwich ELISA - Rat Albumin ELISA Kit (AB108790)

sELISA

Supplier Data

Sandwich ELISA - Rat Albumin ELISA Kit (AB108790)

Representative Standard Curve using ab108790.

sELISA

Lab

Sandwich ELISA - Rat Albumin ELISA Kit (AB108790)

Rat albumin measured in various samples showing quantity (microgram) per mL of tested sample

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Rat

Assay type

Competitive

Sensitivity

= 0.74 µg/mL

Range

1.563 - 12.5 µg/mL

Assay time

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Abcam's Albumin Rat in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of rat Albumin in plasma and serum.

An Albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Albumin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Albumin captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.9" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.8" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108790-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108790-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"1X Biotinylated Rat Albumin Protein (Lyophilized)", "number":"AB108790-CMP01", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108790-CMP09", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108790-CMP08", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108790-CMP07", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Albumin Standard", "number":"AB108790-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Albumin Microplate (12 x 8 well strips)", "number":"AB108790-CMP05", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108790-CMP03", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB108790-CMP02", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Albumin also known as ALB is a major plasma protein with a molecular mass of approximately 66.5 kDa. It predominantly gets synthesized in the liver and found abundantly in blood plasma. Albumin serves multiple functions including maintaining osmotic pressure and acting as a carrier protein for various endogenous substances like hormones fatty acids and bilirubin.

Biological function summary

Albumin plays a critical role in transporting small molecules like metabolites and drugs through the circulatory system. It is not a part of any complex but functions independently to bind transport and release various ligands. Albumin's affinity for hydrophobic molecules makes it essential for they to be solubilized in blood for proper metabolism and excretion.

Pathways

More than five binding sites on albumin facilitate its role in the fatty acid metabolism and renin-angiotensin system pathways. In fatty acid metabolism albumin interacts with transport proteins carrying fatty acids to tissues for energy production. Additionally during the renin-angiotensin system albumin modulates blood pressure through its interaction with angiotensin II which can influence vasoconstriction.

Researchers link albumin with nephrotic syndrome and liver cirrhosis. In nephrotic syndrome low levels of albumin (albumin low) can cause edema due to imbalanced osmotic pressure. In liver cirrhosis a reduction in albumin synthesis signifies liver damage. Albumin's interaction with proteins like angiotensin II correlates with complications such as hypertension further establishing the protein's role in health and disease contexts.

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Product protocols

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Target data

Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (By similarity). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (By similarity). Does not prevent iron uptake by the bacterial siderophore aerobactin (By similarity).

See full target information Alb

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Nephron 147:281-300 PubMed36265461

2022

Responses in Blood Pressure and Kidney Function to Soluble Guanylyl Cyclase Stimulation or Activation in Normal and Diabetic Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Rohit Patel,Yiling Fu,Ser Khang,Agnes M Benardeau,Scott C Thomson,Volker Vallon

Clinical science (London, England : 1979) 136:675-694 PubMed35441670

2022

Differential effects of cyclo-oxygenase 1 and 2 inhibition on angiogenesis inhibitor-induced hypertension and kidney damage.

Applications

Unspecified application

Species

Unspecified reactive species

Katrina M Mirabito Colafella,Daan C H van Dorst,Rugina I Neuman,Leni van Doorn,Karla Bianca Neves,Augusto C Montezano,Ingrid M Garrelds,Richard van Veghel,René de Vries,Estrellita Uijl,Marian C Clahsen-van Groningen,Hans J Baelde,Anton H van den Meiracker,Rhian M Touyz,Willy Visser,A H Jan Danser,Jorie Versmissen

Brain pathology (Zurich, Switzerland) 31:333-345 PubMed33220123

2021

Iron accumulation in the choroid plexus, ependymal cells and CNS parenchyma in a rat strain with low-grade haemolysis of fragile macrocytic red blood cells.

Applications

Unspecified application

Species

Unspecified reactive species

Isabella Wimmer,Cornelia Scharler,Taro Kadowaki,Sophie Hillebrand,Barbara Scheiber-Mojdehkar,Shuichi Ueda,Monika Bradl,Thomas Berger,Hans Lassmann,Simon Hametner

The Journal of clinical investigation 129:4901-4911 PubMed31408444

2019

AAV8-vectored suprachoroidal gene transfer produces widespread ocular transgene expression.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Ding,Jikui Shen,Zibran Hafiz,Sean F Hackett,Raquel Lima E Silva,Mahmood Khan,Valeria E Lorenc,Daiqin Chen,Rishi Chadha,Minie Zhang,Sherri Van Everen,Nicholas Buss,Michele Fiscella,Olivier Danos,Peter A Campochiaro

Frontiers in physiology 8:517 PubMed28798692

2017

Change in the Lipid Transport Capacity of the Liver and Blood during Reproduction in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yufeng Zhang,Christine Kallenberg,Hayden W Hyatt,Andreas N Kavazis,Wendy R Hood

Oxidative medicine and cellular longevity 2014:961326 PubMed25243053

2014

Urinary excretion of neutrophil gelatinase-associated lipocalin in diabetic rats.

Applications

Unspecified application

Species

Unspecified reactive species

Abraham Said Arellano-Buendía,Fernando Enrique García-Arroyo,Magdalena Cristóbal-García,María Lilia Loredo-Mendoza,Edilia Tapia-Rodríguez,Laura Gabriela Sánchez-Lozada,Horacio Osorio-Alonso

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Rat Albumin ELISA Kit

Sandwich ELISA - Rat Albumin ELISA Kit (AB108790)

Sandwich ELISA - Rat Albumin ELISA Kit (AB108790)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Secondary Antibodies

Assay Kits

Assay Kits

Primary Antibodies

Reactivity data

Product details

Precision

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise