Rat Albumin ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat Albumin with a sensitivity of 94.64 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Publications
Filter by
- Life (Basel, Switzerland) 13:2023Seeds Confer Kidney Protection against Early Life Exposure to Acephate by Regulating Oxidative Insult and Activating Autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAfoua Mufti,Anouar Feriani,María Del Mar Contreras,Saber Nehdi,Najla Hfaeidh,Nizar Tlili,Abdel Halim HarrathPubMed 38137855
- Chemical biology & drug design 103:e143862023Protective effects of baicalin on diethyl nitrosamine-induced liver cirrhosis by suppressing oxidative stress and inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui Wang,Yufei Chang,Xiao Liu,Linan Liu,Mingxi Hua,Ang LiPubMed 37923393
- American journal of physiology. Cell physiology 324:C951-C9622023Renoprotective effects of empagliflozin are linked to activation of the tubuloglomerular feedback mechanism and blunting of the complement system.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Chen,Denis Delić,Yaochen Cao,Linghong Shen,Qin Shao,Zheyu Zhang,Hongwei Wu,Ahmed A Hasan,Christoph Reichetzeder,Mohamed M S Gaballa,Bernhard K Krämer,Thomas Klein,Lianghong Yin,Ben He,Stanislao Morgera,Berthold HocherPubMed 36779666
- ALTEX 40:83-1022022A rodent thyroid-liver chip to capture thyroid toxicity on organ function level.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDiana Karwelat,Julia Kühnlenz,Thomas Steger-Hartmann,Remi Bars,Helen Tinwell,Uwe Marx,Sophie Bauer,Oliver Born,Marian RaschkePubMed 35791291
- BMC nephrology 23:1172022Regulation of SARS CoV-2 host factors in the kidney and heart in rats with 5/6 nephrectomy-effects of salt, ARB, DPP4 inhibitor and SGLT2 blocker.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYingquan Xiong,Denis Delic,Shufei Zeng,Xin Chen,Chang Chu,Ahmed A Hasan,Bernhard K Krämer,Thomas Klein,Lianghong Yin,Berthold HocherPubMed 35331159
- IET nanobiotechnology 15:473-4832021Alleviation of diabetic nephropathy by zinc oxide nanoparticles in streptozotocin-induced type 1 diabetes in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGhada Alomari,Bahaa Al-Trad,Salehhuddin Hamdan,Alaa A A Aljabali,Mazhar Salim Al Zoubi,Khalid Al-Batanyeh,Janti Qar,Gregory J Eaton,Almuthanna K Alkaraki,Walhan Alshaer,Saja Haifawi,Khairunadwa Jemon,Dinesh Kumar Chellappan,Kamal Dua,Murtaza M TambuwalaPubMed 34694755
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 312.5 - 20000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 94.64 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (By similarity). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (By similarity). Does not prevent iron uptake by the bacterial siderophore aerobactin (By similarity).
Alternative names
Albumin, Alb
What's included?
Recommended products
Rat Albumin ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat Albumin with a sensitivity of 94.64 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 312.5 - 20000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 94.64 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 9 | C.V. 3.7 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 3.3 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 109 | Range 108 - 111 % |
Sample type EDTA Plasma | Average % = 107 | Range 106 - 108 % |
Sample type Urine | Average % = 113 | Range 111 - 116 % |
Sample type Heparin Plasma | Average % = 104 | Range 102 - 106 % |
Sample type Citrate plasma | Average % = 113 | Range 108 - 117 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Rat Albumin ELISA Kit (ab235642) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Albumin protein in cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat Albumin with 94.64 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Albumin also known as ALB is a major plasma protein with a molecular mass of approximately 66.5 kDa. It predominantly gets synthesized in the liver and found abundantly in blood plasma. Albumin serves multiple functions including maintaining osmotic pressure and acting as a carrier protein for various endogenous substances like hormones fatty acids and bilirubin.
- Biological function summary
Albumin plays a critical role in transporting small molecules like metabolites and drugs through the circulatory system. It is not a part of any complex but functions independently to bind transport and release various ligands. Albumin's affinity for hydrophobic molecules makes it essential for they to be solubilized in blood for proper metabolism and excretion.
- Pathways
More than five binding sites on albumin facilitate its role in the fatty acid metabolism and renin-angiotensin system pathways. In fatty acid metabolism albumin interacts with transport proteins carrying fatty acids to tissues for energy production. Additionally during the renin-angiotensin system albumin modulates blood pressure through its interaction with angiotensin II which can influence vasoconstriction.
- Associated diseases and disorders
Researchers link albumin with nephrotic syndrome and liver cirrhosis. In nephrotic syndrome low levels of albumin (albumin low) can cause edema due to imbalanced osmotic pressure. In liver cirrhosis a reduction in albumin synthesis signifies liver damage. Albumin's interaction with proteins like angiotensin II correlates with complications such as hypertension further establishing the protein's role in health and disease contexts.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Sandwich ELISA - Rat Albumin ELISA Kit (ab235642)
Example of rat Albumin standard curve in Sample Diluent NS.
The Albumin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Rat Albumin ELISA Kit (ab235642)
Interpolated concentrations of native Albumin in rat serum and plasma samples.
The concentrations of Albumin were measured in duplicates, interpolated from the Albumin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:2 million, plasma (heparin) 1:8 million, plasma (EDTA) 1:4 million, and plasma (citrate) 1:4 million. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Albumin concentration in the neat samples was determined to be 32 mg/mL in serum, 29 mg/mL in plasma (heparin), 26 mg/mL in plasma (EDTA), and 23 mg/mL in plasma (citrate).
Sandwich ELISA - Rat Albumin ELISA Kit (ab235642)
Interpolated concentrations of native Albumin in rat urine.
The concentrations of Albumin were measured in duplicate and interpolated from the Albumin standard curve and corrected for sample dilution. Undiluted samples are 1:1,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Albumin concentration in the neat sample was determined to be 18 μg/mL.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com