AB235642

Rat Albumin ELISA Kit

Recombinant
SimpleStep
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(6 Publications)

Rat Albumin ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat Albumin with a sensitivity of 94.64 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Albumin, Alb

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ELISA - Rat Albumin ELISA Kit (AB235642)

ELISA

Supplier Data

ELISA - Rat Albumin ELISA Kit (AB235642)

sELISA

Supplier Data

Sandwich ELISA - Rat Albumin ELISA Kit (AB235642)

Example of rat Albumin standard curve in Sample Diluent NS.

The Albumin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Rat Albumin ELISA Kit (AB235642)

Interpolated concentrations of native Albumin in rat serum and plasma samples.

The concentrations of Albumin were measured in duplicates, interpolated from the Albumin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 2 million, plasma (heparin) 1 : 8 million, plasma (EDTA) 1 : 4 million, and plasma (citrate) 1 : 4 million. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Albumin concentration in the neat samples was determined to be 32 mg/mL in serum, 29 mg/mL in plasma (heparin), 26 mg/mL in plasma (EDTA), and 23 mg/mL in plasma (citrate).

sELISA

Supplier Data

Sandwich ELISA - Rat Albumin ELISA Kit (AB235642)

Interpolated concentrations of native Albumin in rat urine.

The concentrations of Albumin were measured in duplicate and interpolated from the Albumin standard curve and corrected for sample dilution. Undiluted samples are 1 : 1,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Albumin concentration in the neat sample was determined to be 18 μg/mL.

Key facts

Detection method

Colorimetric

Sample types

Urine, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 94.64 pg/mL

Range

312.5 - 20000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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AB235628

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Why is this recommended?

We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

Rat Albumin ELISA Kit ab235642 is a rapid single-wash 90-min Sandwich ELISA to measure Rat Albumin in citrate plasma, EDTA plasma, heparin plasma, serum, urine. This SimpleStep sensitivity is 94.64 pg/mL.

How the assay works

Rat Albumin SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Rat Albumin ELISA Kit ab235642 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Albumin antibody [EPR21023-208] - BSA and Azide free (Capture) ab245044
- Anti-Albumin antibody [EPR21023-39] - BSA and Azide free (Detector) ab245044

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.3" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 9, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.7" } ]

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Recovery

[ { "sample": "Serum", "range": "108 - 111 %", "average": "= 109" }, { "sample": "EDTA Plasma", "range": "106 - 108 %", "average": "= 107" }, { "sample": "Urine", "range": "111 - 116 %", "average": "= 113" }, { "sample": "Heparin Plasma", "range": "102 - 106 %", "average": "= 104" }, { "sample": "Citrate plasma", "range": "108 - 117 %", "average": "= 113" } ]

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What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab235642-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB235642-CMP04" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB235642-CMP10" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB235642-CMP05" }, { "size":"1 x 6000 µL", "name":"10X Rat Albumin Capture Antibody", "number":"AB235642-CMP06" }, { "size":"2 x 10 Vial", "name":"Rat Albumin Lyophilized Recombinant Protein", "number":"AB235642-CMP08" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB235642-CMP01" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB235642-CMP02" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB235642-CMP03" }, { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB235642-CMP09" }, { "size":"1 x 6000 µL", "name":"10X Rat Albumin Detector Antibody", "number":"AB235642-CMP07" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab235642-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB235642-CMP04", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB235642-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB235642-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB235642-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB235642-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB235642-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB235642-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat Albumin Capture Antibody", "number":"AB235642-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rat Albumin Lyophilized Recombinant Protein", "number":"AB235642-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat Albumin Detector Antibody", "number":"AB235642-CMP07", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Albumin also known as ALB is a major plasma protein with a molecular mass of approximately 66.5 kDa. It predominantly gets synthesized in the liver and found abundantly in blood plasma. Albumin serves multiple functions including maintaining osmotic pressure and acting as a carrier protein for various endogenous substances like hormones fatty acids and bilirubin.

Biological function summary

Albumin plays a critical role in transporting small molecules like metabolites and drugs through the circulatory system. It is not a part of any complex but functions independently to bind transport and release various ligands. Albumin's affinity for hydrophobic molecules makes it essential for they to be solubilized in blood for proper metabolism and excretion.

Pathways

More than five binding sites on albumin facilitate its role in the fatty acid metabolism and renin-angiotensin system pathways. In fatty acid metabolism albumin interacts with transport proteins carrying fatty acids to tissues for energy production. Additionally during the renin-angiotensin system albumin modulates blood pressure through its interaction with angiotensin II which can influence vasoconstriction.

Researchers link albumin with nephrotic syndrome and liver cirrhosis. In nephrotic syndrome low levels of albumin (albumin low) can cause edema due to imbalanced osmotic pressure. In liver cirrhosis a reduction in albumin synthesis signifies liver damage. Albumin's interaction with proteins like angiotensin II correlates with complications such as hypertension further establishing the protein's role in health and disease contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (By similarity). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (By similarity). Does not prevent iron uptake by the bacterial siderophore aerobactin (By similarity).

See full target information Alb

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Life (Basel, Switzerland) 13: PubMed38137855

2023

Seeds Confer Kidney Protection against Early Life Exposure to Acephate by Regulating Oxidative Insult and Activating Autophagy.

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Afoua Mufti,Anouar Feriani,María Del Mar Contreras,Saber Nehdi,Najla Hfaeidh,Nizar Tlili,Abdel Halim Harrath

Chemical biology & drug design 103:e14386 PubMed37923393

2023

Protective effects of baicalin on diethyl nitrosamine-induced liver cirrhosis by suppressing oxidative stress and inflammation.

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Hui Wang,Yufei Chang,Xiao Liu,Linan Liu,Mingxi Hua,Ang Li

American journal of physiology. Cell physiology 324:C951-C962 PubMed36779666

2023

Renoprotective effects of empagliflozin are linked to activation of the tubuloglomerular feedback mechanism and blunting of the complement system.

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Xin Chen,Denis Delić,Yaochen Cao,Linghong Shen,Qin Shao,Zheyu Zhang,Hongwei Wu,Ahmed A Hasan,Christoph Reichetzeder,Mohamed M S Gaballa,Bernhard K Krämer,Thomas Klein,Lianghong Yin,Ben He,Stanislao Morgera,Berthold Hocher

ALTEX 40:83-102 PubMed35791291

2022

A rodent thyroid-liver chip to capture thyroid toxicity on organ function level.

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Diana Karwelat,Julia Kühnlenz,Thomas Steger-Hartmann,Remi Bars,Helen Tinwell,Uwe Marx,Sophie Bauer,Oliver Born,Marian Raschke

BMC nephrology 23:117 PubMed35331159

2022

Regulation of SARS CoV-2 host factors in the kidney and heart in rats with 5/6 nephrectomy-effects of salt, ARB, DPP4 inhibitor and SGLT2 blocker.

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Yingquan Xiong,Denis Delic,Shufei Zeng,Xin Chen,Chang Chu,Ahmed A Hasan,Bernhard K Krämer,Thomas Klein,Lianghong Yin,Berthold Hocher

IET nanobiotechnology 15:473-483 PubMed34694755

2021

Alleviation of diabetic nephropathy by zinc oxide nanoparticles in streptozotocin-induced type 1 diabetes in rats.

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Ghada Alomari,Bahaa Al-Trad,Salehhuddin Hamdan,Alaa A A Aljabali,Mazhar Salim Al Zoubi,Khalid Al-Batanyeh,Janti Qar,Gregory J Eaton,Almuthanna K Alkaraki,Walhan Alshaer,Saja Haifawi,Khairunadwa Jemon,Dinesh Kumar Chellappan,Kamal Dua,Murtaza M Tambuwala

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

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