Rat Alpha 1 Acid Glycoprotein / AGP ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Rat Alpha 1 Acid Glycoprotein / AGP in Rat in Plasma, Serum samples.
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- Journal of orthopaedic surgery and research 18:5222023The early infection characterization of septic arthritis by Staphylococcus aureus after anterior cruciate ligament reconstruction in a novel rat model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKai Tong,Jian Wei,Zilin Liu,Xiaoming Yang,Yong HuPubMed 37481547
- Diabetes 65:1630-412016The Acute-Phase Protein Orosomucoid Regulates Food Intake and Energy Homeostasis via Leptin Receptor Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Sun et. al.PubMed 27207522
Key facts
- Detection method
Colorimetric
- Sample types
Plasma, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Rat
- Range
12.5 - 400 ng/mL
- Sensitivity
= 2.1663 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Functions as transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain (By similarity). Appears to function in modulating the activity of the immune system during the acute-phase reaction.
Alternative names
Alpha-1-acid glycoprotein, Orosomucoid, OMD, Orm1
What's included?
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Rat Alpha 1 Acid Glycoprotein / AGP ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Rat Alpha 1 Acid Glycoprotein / AGP in Rat in Plasma, Serum samples.
Key facts
- Detection method
Colorimetric
- Sample types
Plasma, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Rat
- Range
12.5 - 400 ng/mL
- Assay Platform
Microplate
- Sensitivity
= 2.1663 ng/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % > 85 | Range |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Abcam's Rat Alpha 1 Acid Glycoprotein / AGP ELISA kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Alpha 1 Acid Glycoprotein / AGP levels in rat serum and plasma samples.
In this assay the Alpha 1 Acid Glycoprotein / AGP present in samples reacts with the anti-Alpha 1 Acid Glycoprotein / AGP antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-Alpha 1 Acid Glycoprotein / AGP antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound Alpha 1 Acid Glycoprotein / AGP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Alpha 1 Acid Glycoprotein / AGP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Alpha 1 Acid Glycoprotein / AGP in the test sample. The quantity of Alpha 1 Acid Glycoprotein / AGP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Alpha 1 Acid Glycoprotein also known as AGP or Alpha 1 Glycoprotein is a plasma protein with a molecular weight of approximately 41 to 43 kDa. This protein circulates primarily in the bloodstream and is produced mainly by the liver. AGP is known for its highly glycosylated structure carrying several branched oligosaccharide chains. It exhibits a significant capacity to bind various endogenous and exogenous lipophilic substances influencing their transport and distribution in the body.
- Biological function summary
AGP plays a role in modulating the immune response and maintaining homeostasis. As an acute-phase protein its levels rise significantly during inflammation infection and trauma where it acts to modulate the immune system. AGP does not exist as part of a larger protein complex but interacts with plasma lipoproteins and other glycoproteins aiding in its function of maintaining vascular integrity during acute-phase reactions.
- Pathways
Alpha 1 Glycoprotein serves as an important component in the inflammatory response and drug-binding pathways. In the inflammatory pathway AGP interacts with cytokines and acute-phase reactants adjusting the body’s inflammatory processes. It is also involved in the binding and distribution of drugs interacting with albumin and other transport proteins to influence the pharmacokinetics of many medications.
- Associated diseases and disorders
AGP levels change in response to conditions like rheumatoid arthritis and liver diseases. In rheumatoid arthritis its increased levels correlate with disease activity and inflammation. In liver diseases the production and plasma concentration of AGP can reflect hepatic synthesis capability. AGP's interaction with cytokines in these conditions often links it to elevated levels of other acute-phase proteins like C-reactive protein reflecting its participation in the pathophysiological processes of inflammation.
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Sandwich ELISA - Rat Alpha 1 Acid Glycoprotein / AGP ELISA Kit (ab157729)
Representative standard curve using ab157729 Alpha 1 Acid Glycoprotein / AGP Rat ELISA Kit
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