AB157729

Rat Alpha 1 Acid Glycoprotein / AGP ELISA Kit

Name: Rat Alpha 1 Acid Glycoprotein / AGP ELISA Kit (ab157729) | Abcam
Brand: Abcam Limited
SKU: AB157729

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(2 Publications)

Rat Alpha 1 Acid Glycoprotein / AGP ELISA Kit is a sandwich ELISA designed to quantify Rat Alpha 1 Acid Glycoprotein / AGP with a sensitivity of 2.1663 ng/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 12.5 - 400 ng/mL

View Alternative Names

Alpha-1-acid glycoprotein, Orosomucoid, OMD, Orm1

1 Images

Sandwich ELISA - Rat Alpha 1 Acid Glycoprotein / AGP ELISA Kit (AB157729)

sELISA

Supplier Data

Sandwich ELISA - Rat Alpha 1 Acid Glycoprotein / AGP ELISA Kit (AB157729)

Representative standard curve using ab157729 Alpha 1 Acid Glycoprotein / AGP Rat ELISA Kit

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 2.1663 ng/mL

Range

12.5 - 400 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Rat Alpha 1 Acid Glycoprotein / AGP ELISA Kit ab157729 is a sandwich ELISA to measure Rat Alpha 1 Acid Glycoprotein / AGP in serum, plasma with a sensitivity of 2.1663 ng/ml.

How the assay works

In this assay the Alpha 1 Acid Glycoprotein / AGP present in samples reacts with the anti-Alpha 1 Acid Glycoprotein / AGP antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-Alpha 1 Acid Glycoprotein / AGP antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound Alpha 1 Acid Glycoprotein / AGP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Alpha 1 Acid Glycoprotein / AGP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Alpha 1 Acid Glycoprotein / AGP in the test sample. The quantity of Alpha 1 Acid Glycoprotein / AGP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Rat Alpha 1 Acid Glycoprotein / AGP ELISA Kit ab157729 protocol summary

1. Add standard or sample to each well used. Incubate at room temperature.
2. Aspirate and wash each well.
3. Add prepared HRP labeled secondary detector antibody. Incubate at room temperature
4. Aspirate and wash each well.
5. Add Chromogen Substrate Solution to each well.
6. Immediately begin recording the color development

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]

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Recovery

[ { "sample": "Serum", "range": null, "average": "> 85" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha 1 Acid Glycoprotein also known as AGP or Alpha 1 Glycoprotein is a plasma protein with a molecular weight of approximately 41 to 43 kDa. This protein circulates primarily in the bloodstream and is produced mainly by the liver. AGP is known for its highly glycosylated structure carrying several branched oligosaccharide chains. It exhibits a significant capacity to bind various endogenous and exogenous lipophilic substances influencing their transport and distribution in the body.

Biological function summary

AGP plays a role in modulating the immune response and maintaining homeostasis. As an acute-phase protein its levels rise significantly during inflammation infection and trauma where it acts to modulate the immune system. AGP does not exist as part of a larger protein complex but interacts with plasma lipoproteins and other glycoproteins aiding in its function of maintaining vascular integrity during acute-phase reactions.

Pathways

Alpha 1 Glycoprotein serves as an important component in the inflammatory response and drug-binding pathways. In the inflammatory pathway AGP interacts with cytokines and acute-phase reactants adjusting the body's inflammatory processes. It is also involved in the binding and distribution of drugs interacting with albumin and other transport proteins to influence the pharmacokinetics of many medications.

AGP levels change in response to conditions like rheumatoid arthritis and liver diseases. In rheumatoid arthritis its increased levels correlate with disease activity and inflammation. In liver diseases the production and plasma concentration of AGP can reflect hepatic synthesis capability. AGP's interaction with cytokines in these conditions often links it to elevated levels of other acute-phase proteins like C-reactive protein reflecting its participation in the pathophysiological processes of inflammation.

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Product protocols

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Target data

Functions as a transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain (By similarity). Appears to function in modulating the activity of the immune system during the acute-phase reaction.

See full target information Orm1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of orthopaedic surgery and research 18:522 PubMed37481547

2023

The early infection characterization of septic arthritis by Staphylococcus aureus after anterior cruciate ligament reconstruction in a novel rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Tong,Jian Wei,Zilin Liu,Xiaoming Yang,Yong Hu

Diabetes 65:1630-41 PubMed27207522

2016

The Acute-Phase Protein Orosomucoid Regulates Food Intake and Energy Homeostasis via Leptin Receptor Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Sun,Yili Yang,Zhen Qin,Jinya Cai,Xiuming Guo,Yun Tang,Jingjing Wan,Ding-Feng Su,Xia Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rat Alpha 1 Acid Glycoprotein / AGP ELISA Kit

Sandwich ELISA - Rat Alpha 1 Acid Glycoprotein / AGP ELISA Kit (AB157729)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay Platform

Complementary Products

Assay Kits

Secondary Antibodies

Assay Kits

Primary Antibodies

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise