Rat alpha 2 Macroglobulin ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat alpha 2 Macroglobulin with a sensitivity of 14 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media, Urine
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 65.625 - 4200 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 14 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
Alternative names
A2m1, A2m, Alpha-2-macroglobulin, Alpha-2-M
What's included?
Recommended products
Rat alpha 2 Macroglobulin ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat alpha 2 Macroglobulin with a sensitivity of 14 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media, Urine
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 65.625 - 4200 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 14 pg/mL
- General recovery
- 94%
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. 3.5 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 2.2 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 94 | Range 91 - 96 % |
Sample type Citrate plasma | Average % = 110 | Range 109 - 113 % |
Sample type EDTA Plasma | Average % = 104 | Range 95 - 114 % |
Sample type Heparin Plasma | Average % = 96 | Range 91 - 100 % |
Sample type Cell culture media | Average % = 84 | Range 83 - 85 % |
Sample type Urine | Average % = 101 | Range 94 - 110 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Rat alpha 2 Macroglobulin SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of alpha 2 Macroglobulin protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture media, Urine. Quantitate Rat alpha 2 Macroglobulin with 14 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Alpha-2-macroglobulin also known as alpha-2-M and A2M is a large glycoprotein with a molecular mass of approximately 725 kDa. It is expressed in the liver and is found in high concentrations in the blood plasma. This protein functions mechanically as a broad-spectrum protease inhibitor. It can trap proteases within its structure preventing them from accessing substrates. Additionally alpha-2-macroglobulin can bind to cytokines and growth factors altering their availability and activities.
- Biological function summary
Alpha-2-macroglobulin plays an important role in maintaining homeostasis by regulating protease activities and modulating immune system reactions. It does not form a permanent complex but can transiently associate with the proteases and various molecules mentioned. By interacting with proteases it controls processes like coagulation and fibrinolysis and participates in the clearance of proteinases and growth factors from the circulation. It influences cell signaling pathways and inflammatory responses highlighting its significance in maintaining balance within the body.
- Pathways
Alpha-2-macroglobulin is an important component in the immune response and coagulation pathways. In the complement system it binds with complement components to regulate their activities. It associates with proteins such as plasmin and thrombin which are important for blood clotting and fibrinolysis. These relationships emphasize the protein's involvement in both protective mechanisms against pathogens and the regulation of blood coagulation and breakdown connecting the immune and hemostatic systems.
- Associated diseases and disorders
Alpha-2-macroglobulin has been linked to Alzheimer's disease and liver diseases. In Alzheimer's altered levels of this protein might affect the amyloid-beta peptide clearance due to its protease inhibition and binding properties. It associates with proteins like lipoprotein receptor-related protein-1 (LRP1) influencing amyloid-beta metabolism. In liver diseases its levels can indicate liver dysfunction or inflammation given its synthesis in the liver and can relate to proteins involved in inflammation such as cytokines. Its modulation of protease activity makes it a critical target in understanding these disorders.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
Sandwich ELISA - Rat alpha 2 Macroglobulin ELISA Kit (ab320793)
Example of rat alpha 2 Macroglobulin standard curve in Sample Diluent NS. Example of rat alpha 2 Macroglobulin standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Rat alpha 2 Macroglobulin ELISA Kit (ab320793)
Interpolated concentrations of rat alpha 2 Macroglobulin in serum, plasma (citrate), plasma (EDTA), and plasma (heparin). Interpolated concentration of native alpha 2 Macroglobulin was measured in duplicate at different sample concentrations. Undiluted samples are as follows: serum 1:20,000, plasma (citrate) 1:10,000, plasma (EDTA) 1:10,000, and plasma (heparin) 1:10,000. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Rat alpha 2 Macroglobulin ELISA Kit (ab320793)
Interpolated concentrations of rat alpha 2 Macroglobulin in urine. Interpolated concentration of native alpha 2 Macroglobulin was measured in duplicate at different sample concentrations. Undiluted sample is 50% urine. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Rat alpha 2 Macroglobulin ELISA Kit (ab320793)
Interpolated concentrations of alpha 2 Macroglobulin in rat, human, mouse, cow, and monkey serum samples. Serial dilutions of rat, human, mouse, cow, and monkey serum samples were measured in duplicate and interpolated from the rat standard curve. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Rat alpha 2 Macroglobulin ELISA Kit (ab320793)
Example of rat alpha 2 Macroglobulin standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Example of rat alpha 2 Macroglobulin standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Rat alpha 2 Macroglobulin ELISA Kit (ab320793)
Interpolated concentrations of rat alpha 2 Macroglobulin in serum in 96-well vs. 384-well plates. Interpolated concentration of native alpha 2 Macroglobulin was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted sample is 1:20,000 serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com