AB320793

Rat alpha 2 Macroglobulin ELISA Kit

Recombinant
SimpleStep
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Rat alpha 2 Macroglobulin ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat alpha 2 Macroglobulin with a sensitivity of 14 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput

View Alternative Names

A2m1, A2m, Alpha-2-macroglobulin, Alpha-2-M

6 Images

Sandwich ELISA - Rat alpha 2 Macroglobulin ELISA Kit (AB320793)

sELISA

Supplier Data

Sandwich ELISA - Rat alpha 2 Macroglobulin ELISA Kit (AB320793)

Interpolated concentrations of rat alpha 2 Macroglobulin in serum, plasma (citrate), plasma (EDTA), and plasma (heparin). Interpolated concentration of native alpha 2 Macroglobulin was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 1 : 20,000, plasma (citrate) 1 : 10,000, plasma (EDTA) 1 : 10,000, and plasma (heparin) 1 : 10,000. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Rat alpha 2 Macroglobulin ELISA Kit (AB320793)

Interpolated concentrations of rat alpha 2 Macroglobulin in serum in 96-well vs. 384-well plates. Interpolated concentration of native alpha 2 Macroglobulin was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted sample is 1 : 20,000 serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Rat alpha 2 Macroglobulin ELISA Kit (AB320793)

Interpolated concentrations of rat alpha 2 Macroglobulin in urine. Interpolated concentration of native alpha 2 Macroglobulin was measured in duplicate at different sample concentrations. Undiluted sample is 50% urine. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Rat alpha 2 Macroglobulin ELISA Kit (AB320793)

Interpolated concentrations of alpha 2 Macroglobulin in rat, human, mouse, cow, and monkey serum samples. Serial dilutions of rat, human, mouse, cow, and monkey serum samples were measured in duplicate and interpolated from the rat standard curve. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Rat alpha 2 Macroglobulin ELISA Kit (AB320793)

Example of rat alpha 2 Macroglobulin standard curve in Sample Diluent NS. Example of rat alpha 2 Macroglobulin standard curve. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Rat alpha 2 Macroglobulin ELISA Kit (AB320793)

Example of rat alpha 2 Macroglobulin standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Example of rat alpha 2 Macroglobulin standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media, Urine

Reacts with

Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 14 pg/mL

Range

65.625 - 4200 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Rat alpha 2 Macroglobulin SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of alpha 2 Macroglobulin protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture media, Urine. Quantitate Rat alpha 2 Macroglobulin with 14 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.5" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.2" } ]

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Recovery

[ { "sample": "Serum", "range": "91 - 96 %", "average": "= 94" }, { "sample": "Citrate plasma", "range": "109 - 113 %", "average": "= 110" }, { "sample": "EDTA Plasma", "range": "95 - 114 %", "average": "= 104" }, { "sample": "Heparin Plasma", "range": "91 - 100 %", "average": "= 96" }, { "sample": "Cell culture media", "range": "83 - 85 %", "average": "= 84" }, { "sample": "Urine", "range": "94 - 110 %", "average": "= 101" } ]

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What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab320793-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB320793-CMP08" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB320793-CMP06" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB320793-CMP07" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB320793-CMP09" }, { "size":"20 x 1 Vial", "name":"Rat alpha 2 Macroglobulin Lyophilized Recombinant Protein", "number":"AB320793-CMP03" }, { "size":"10 x 600 µL", "name":"10X Rat alpha 2 Macroglobulin Detector Antibody", "number":"AB320793-CMP02" }, { "size":"10 x 600 µL", "name":"10X Rat alpha 2 Macroglobulin Capture Antibody", "number":"AB320793-CMP01" }, { "size":"10 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB320793-CMP04" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB320793-CMP05" }, { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB320793-CMP10" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab320793-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB320793-CMP08" }, { "size":"2 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB320793-CMP06" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB320793-CMP07" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB320793-CMP10" }, { "size":"2 x 1 Vial", "name":"Rat alpha 2 Macroglobulin Lyophilized Recombinant Protein", "number":"AB320793-CMP03" }, { "size":"1 x 600 µL", "name":"10X Rat alpha 2 Macroglobulin Detector Antibody", "number":"AB320793-CMP02" }, { "size":"1 x 600 µL", "name":"10X Rat alpha 2 Macroglobulin Capture Antibody", "number":"AB320793-CMP01" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB320793-CMP04" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB320793-CMP11" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB320793-CMP05" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab320793-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB320793-CMP08", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB320793-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB320793-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB320793-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rat alpha 2 Macroglobulin Lyophilized Recombinant Protein", "number":"AB320793-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat alpha 2 Macroglobulin Detector Antibody", "number":"AB320793-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat alpha 2 Macroglobulin Capture Antibody", "number":"AB320793-CMP01", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB320793-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB320793-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB320793-CMP10", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-2-macroglobulin also known as alpha-2-M and A2M is a large glycoprotein with a molecular mass of approximately 725 kDa. It is expressed in the liver and is found in high concentrations in the blood plasma. This protein functions mechanically as a broad-spectrum protease inhibitor. It can trap proteases within its structure preventing them from accessing substrates. Additionally alpha-2-macroglobulin can bind to cytokines and growth factors altering their availability and activities.

Biological function summary

Alpha-2-macroglobulin plays an important role in maintaining homeostasis by regulating protease activities and modulating immune system reactions. It does not form a permanent complex but can transiently associate with the proteases and various molecules mentioned. By interacting with proteases it controls processes like coagulation and fibrinolysis and participates in the clearance of proteinases and growth factors from the circulation. It influences cell signaling pathways and inflammatory responses highlighting its significance in maintaining balance within the body.

Pathways

Alpha-2-macroglobulin is an important component in the immune response and coagulation pathways. In the complement system it binds with complement components to regulate their activities. It associates with proteins such as plasmin and thrombin which are important for blood clotting and fibrinolysis. These relationships emphasize the protein's involvement in both protective mechanisms against pathogens and the regulation of blood coagulation and breakdown connecting the immune and hemostatic systems.

Alpha-2-macroglobulin has been linked to Alzheimer's disease and liver diseases. In Alzheimer's altered levels of this protein might affect the amyloid-beta peptide clearance due to its protease inhibition and binding properties. It associates with proteins like lipoprotein receptor-related protein-1 (LRP1) influencing amyloid-beta metabolism. In liver diseases its levels can indicate liver dysfunction or inflammation given its synthesis in the liver and can relate to proteins involved in inflammation such as cytokines. Its modulation of protease activity makes it a critical target in understanding these disorders.

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Product protocols

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Target data

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

See full target information A2m

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rat alpha 2 Macroglobulin ELISA Kit

Sandwich ELISA - Rat alpha 2 Macroglobulin ELISA Kit (AB320793)

Sandwich ELISA - Rat alpha 2 Macroglobulin ELISA Kit (AB320793)

Sandwich ELISA - Rat alpha 2 Macroglobulin ELISA Kit (AB320793)

Sandwich ELISA - Rat alpha 2 Macroglobulin ELISA Kit (AB320793)

Sandwich ELISA - Rat alpha 2 Macroglobulin ELISA Kit (AB320793)

Sandwich ELISA - Rat alpha 2 Macroglobulin ELISA Kit (AB320793)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Secondary Antibodies

Assay Kits

Assay Kits

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise