AB323686

Rat Alpha fetoprotein (AFP) ELISA Kit

Recombinant
SimpleStep
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Rat Alpha fetoprotein (AFP) ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat Alpha fetoprotein (AFP) with a sensitivity of 13.364 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, Afp

5 Images

Sandwich ELISA - Rat Alpha fetoprotein (AFP) ELISA Kit (AB323686)

sELISA

Supplier Data

Sandwich ELISA - Rat Alpha fetoprotein (AFP) ELISA Kit (AB323686)

Example of rat Alpha fetoprotein standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Rat Alpha fetoprotein (AFP) ELISA Kit (AB323686)

Interpolated concentration of native Alpha fetoprotein was measured in duplicate at different sample concentrations. Undiluted samples are as follows : plasma (citrate)1 : 20, plasma (EDTA) 1 : 200, and plasma (heparin) 1 : 5. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Rat Alpha fetoprotein (AFP) ELISA Kit (AB323686)

Interpolated concentration of native Alpha fetoprotein was measured in duplicate at different sample concentrations. Undiluted samples are as follows : pregnant serum 1 : 12,800 and serum 1 : 5. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Rat Alpha fetoprotein (AFP) ELISA Kit (AB323686)

Example of rat Alpha fetoprotein standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Rat Alpha fetoprotein (AFP) ELISA Kit (AB323686)

Interpolated concentration of native Alpha fetoprotein was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 5 serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Urine, Cell culture media

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 13.364 pg/mL

Range

46.875 - 3000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Rat Alpha fetoprotein (AFP) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Alpha fetoprotein (AFP) protein in Serum, Citrate Plasma, EDTA Plasma, Heparin Plasma, Urine, Cell culture media. Quantitate Rat Alpha fetoprotein (AFP) with 13.364 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

style

left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 8.4" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.4" } ]

style

left-column

Recovery

[ { "sample": "Serum", "range": "85 - 97 %", "average": "= 91" }, { "sample": "Citrate plasma", "range": "84 - 85 %", "average": "= 85" }, { "sample": "EDTA Plasma", "range": "100 - 113 %", "average": "= 107" }, { "sample": "Heparin Plasma", "range": "113 - 119 %", "average": "= 115" }, { "sample": "Urine", "range": "86 - 94 %", "average": "= 89" }, { "sample": "Cell culture media", "range": "95 - 107 %", "average": "= 99" } ]

style

left-column

What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab323686-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB323686-CMP08" }, { "size":"20 x 1 Vial", "name":"Rat Alpha fetoprotein (AFP) Lyophilized Recombinant Protein", "number":"AB323686-CMP03" }, { "size":"10 x 600 µL", "name":"10X Rat Alpha fetoprotein (AFP) Detector Antibody", "number":"AB323686-CMP02" }, { "size":"10 x 600 µL", "name":"10X Rat Alpha fetoprotein (AFP) Capture Antibody", "number":"AB323686-CMP01" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB323686-CMP07" }, { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB323686-CMP10" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB323686-CMP09" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB323686-CMP06" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB323686-CMP05" }, { "size":"10 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB323686-CMP04" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab323686-1x384Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Rat Alpha fetoprotein (AFP) Lyophilized Recombinant Protein", "number":"AB323686-CMP03" }, { "size":"1 x 600 µL", "name":"10X Rat Alpha fetoprotein (AFP) Detector Antibody", "number":"AB323686-CMP02" }, { "size":"1 x 600 µL", "name":"10X Rat Alpha fetoprotein (AFP) Capture Antibody", "number":"AB323686-CMP01" }, { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB323686-CMP08" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB323686-CMP07" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB323686-CMP10" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB323686-CMP11" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB323686-CMP06" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB323686-CMP05" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB323686-CMP04" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab323686-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB323686-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rat Alpha fetoprotein (AFP) Lyophilized Recombinant Protein", "number":"AB323686-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat Alpha fetoprotein (AFP) Detector Antibody", "number":"AB323686-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat Alpha fetoprotein (AFP) Capture Antibody", "number":"AB323686-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB323686-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB323686-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB323686-CMP09", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB323686-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB323686-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB323686-CMP04", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

style

left-column

Product protocols

style

left-column

Target data

Binds estrogens, fatty acids and metals.

See full target information Afp

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com