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Rat ALT ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Rat ALT in Serum, EDTA Plasma samples.

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Images

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Rat

Range

15.6 - 1000 ng/mL

Assay time

1h 30m

Sensitivity

= 15.2 ng/mL

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Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles (By similarity).

Alternative names

What's included?

1 x 96 Tests
Components
10X Rat ALT Capture Antibody
1 x 600 µL
10X Rat ALT Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
50X Cell Extraction Enhancer Solution (ab193971)
1 x 1 mL
Antibody Diluent 4BR
1 x 6 mL
Plate Seals
1 x 1 Unit
Rat ALT Lyophilized Recombinant Protein
2 x 1 Vial
Sample Diluent NS (ab193972)
1 x 50 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

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Rat ALT ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Rat ALT in Serum, EDTA Plasma samples.

Alternative names

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Rat

Range

15.6 - 1000 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 15.2 ng/mL

Precision

Intra assay

Sample

Recombinant Protein

n

6

mean

-

SD

-

C.V.

1.9

Recovery

Sample specific recovery

Sample type

Serum

Average %

= 85

Range

78 - 91 %

Sample type

EDTA Plasma

Average %

= 101

Range

90 - 143 %

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Rat ALT ELISA Kit (ab234579) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ALT protein in edta plasma and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat ALT with 15.2 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Sandwich ELISA - Rat ALT ELISA Kit (ab234579), expandable thumbnail

    Sandwich ELISA - Rat ALT ELISA Kit (ab234579)

    Example of rat ALT standard curve in Sample Diluent NS + Enhancer.

    The ALT standard curve was prepared as described in Section 10.

  • Sandwich ELISA - Rat ALT ELISA Kit (ab234579), expandable thumbnail

    Sandwich ELISA - Rat ALT ELISA Kit (ab234579)

    Interpolated concentrations of recombinant ALT protein in rat serum and plasma-EDTA samples.

    The concentrations of ALT were measured in duplicates, interpolated from the ALT standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ALT concentration was determined to be 81.4 μg/mL in serum and 34.9 μg/mL in plasma-EDTA.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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