Sample Prep & Detection Kits
Conjugation kitsPurification kitsSample preparation kitsChromogen kitsIHC kitsChIP kitsAccessory Reagents & Controls
Accessory reagents & controlsBiochemicals
BiochemicalsProteins and Peptides
Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
Learn about all product ranges with our product overviews.
Featured events
Make new connections at our global events.
Our programs
New Lab Program
Get a head start with our exclusive new lab discount. Enjoy 20% off and free shipping for three months.
New Biotech Program
Just starting out? Get 15% off and free shipping to your lab for six months.
Product promise
Peace of mind that all products perform as stated.
Product reviews
Leave reviews, get rewarded and help your community.
Trial program
Try untested species and applications to earn money off your next order.
Product Insider Program
Be the first to know about our latest product launches - and unlock exclusive offers and discounts.
Rat ALT ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Rat ALT in Serum, EDTA Plasma samples.
Colorimetric
Serum, EDTA Plasma
Sandwich (quantitative)
Rat
15.6 - 1000 ng/mL
1h 30m
= 15.2 ng/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles (By similarity).
Alanine aminotransferase 1, ALT1, Glutamate pyruvate transaminase 1, Glutamic--alanine transaminase 1, Glutamic--pyruvic transaminase 1, GPT 1, Gpt1, Aat1, Gpt
Rat ALT ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Rat ALT in Serum, EDTA Plasma samples.
Alanine aminotransferase 1, ALT1, Glutamate pyruvate transaminase 1, Glutamic--alanine transaminase 1, Glutamic--pyruvic transaminase 1, GPT 1, Gpt1, Aat1, Gpt
Colorimetric
Serum, EDTA Plasma
Sandwich (quantitative)
Rat
15.6 - 1000 ng/mL
1h 30m
Pre-coated microplate (12 x 8 well strips)
= 15.2 ng/mL
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Recombinant Protein | n 6 | mean - | SD - | C.V. 1.9 |
Sample type | Average % | Range |
---|---|---|
Sample type Serum | Average % = 85 | Range 78 - 91 % |
Sample type EDTA Plasma | Average % = 101 | Range 90 - 143 % |
Blue Ice
+4°C
+4°C
Rat ALT ELISA Kit (ab234579) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ALT protein in edta plasma and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat ALT with 15.2 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Example of rat ALT standard curve in Sample Diluent NS + Enhancer.
The ALT standard curve was prepared as described in Section 10.
Interpolated concentrations of recombinant ALT protein in rat serum and plasma-EDTA samples.
The concentrations of ALT were measured in duplicates, interpolated from the ALT standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ALT concentration was determined to be 81.4 μg/mL in serum and 34.9 μg/mL in plasma-EDTA.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com