Rat AMH ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat AMH with a sensitivity of 19.2 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 62.5 - 4000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 19.2 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Plays an important role in several reproductive functions. Induces Muellerian duct regression during male fetal sexual differentiation and plays a role in Leydig cell differentiation and function (By similarity). In female acts as a negative regulator of the primordial to primary follicle transition and decreases FSH sensitivity of growing follicles. AMH signals by binding to a specific type-II receptor, AMHR2, that heterodimerizes with type-I receptors (ACVR1 and BMPR1A), and recruiting SMAD proteins that are translocated to the nucleus to regulate target gene expression (By similarity).
Alternative names
Muellerian-inhibiting factor, Anti-Muellerian hormone, Muellerian-inhibiting substance, AMH, MIS, Amh
What's included?
Rat AMH ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat AMH with a sensitivity of 19.2 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 62.5 - 4000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 19.2 pg/mL
- General recovery
- 96%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. = 2.6 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 0 | mean - | SD - | C.V. = 4.8 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 96 | Range 93 - 100 % |
Sample type Citrate plasma | Average % = 100 | Range 98 - 103 % |
Sample type EDTA Plasma | Average % = 91 | Range 83 - 95 % |
Sample type Heparin Plasma | Average % = 97 | Range 96 - 98 % |
Sample type Cell culture media | Average % = 97 | Range 89 - 102 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Rat AMH SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of AMH protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture media. Quantitate Rat AMH with 19.2 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
- Also available in a fully validated 384-well format.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Sandwich ELISA - Rat AMH ELISA Kit (ab323518)
Interpolated concentrations of rat AMH in serum in 96-well vs. 384-well plates.
Interpolated concentration of native AMH was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 8% serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.
Sandwich ELISA - Rat AMH ELISA Kit (ab323518)
Example of rat AMH standard curve in Sample Diluent NS + 1X Enhancer in 96-well vs. 384-well plate.
Example of rat AMH standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Rat AMH ELISA Kit (ab323518)
Interpolated concentrations of rat AMH in serum, plasma (citrate), plasma (EDTA), and plasma (heparin).
Interpolated concentration of native AMH was measured in duplicate at different sample concentrations. Undiluted samples are 8% serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.
Sandwich ELISA - Rat AMH ELISA Kit (ab323518)
Example of rat AMH standard curve in Sample Diluent NS + 1X Enhancer.
Example of rat AMH standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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