AB231923

Rat Arginase1 ELISA Kit

Recombinant
SimpleStep
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(1 Publication)

Rat Arginase1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat Arginase1 with a sensitivity of 22.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Arginase-1, Liver-type arginase, Type I arginase, Arg1

3 Images

Sandwich ELISA - Rat Arginase1 ELISA Kit (AB231923)

sELISA

Supplier Data

Sandwich ELISA - Rat Arginase1 ELISA Kit (AB231923)

Example of rat Arginase1 standard curve in 1X Cell Extraction Buffer PTR.

The Arginase1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Rat Arginase1 ELISA Kit (AB231923)

Interpolated concentrations of native Arginase1 in rat liver lysate based on a 2.5 μg/mL extract load.

The concentrations of Arginase1 were measured in duplicate and interpolated from the Arginase1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Arginase1 concentration was determined to be 1819.14 pg/mL in liver lysate.

sELISA

Supplier Data

Sandwich ELISA - Rat Arginase1 ELISA Kit (AB231923)

Representative image of Rat Arginase1 ELISA Kit (ab231923)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Rat Arginase1 ELISA Kit Capture Antibody

- 10X Rat Arginase1 Detector Antibody

- 10X Rat Arginase1 ELISA Kit Lyophilised Purified Protein (2 vials)

- Rat Arginase1 ELISA Kit 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BR

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Key facts

Detection method

Colorimetric

Sample types

Tissue Lysate

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 22.1 pg/mL

Range

156.25 - 10000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Rat Arginase1 ELISA Kit (ab231923) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Arginase1 protein in tissue lysate. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat Arginase1 with 22.1 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Liver Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.1" }, { "reproducibilityType": "Intra", "sample": "Liver Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" } ]

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Recovery

[ { "sample": "Tissue Lysate", "range": "96 - 102 %", "average": "= 98" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab231923-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB231923-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB231923-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB231923-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB231923-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB231923-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB231923-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB231923-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat Arginase 1 Capture Antibody", "number":"AB231923-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rat Arginase 1 Lyophilized Recombinant Protein", "number":"AB231923-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB231923-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB231923-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat Arginase 1 Detector Antibody", "number":"AB231923-CMP09", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys.. Functions in L-arginine homeostasis in nonhepatic tissues characterized by the competition between nitric oxide synthase (NOS) and arginase for the available intracellular substrate arginine. Arginine metabolism is a critical regulator of innate and adaptive immune responses. Involved in an antimicrobial effector pathway in polymorphonuclear granulocytes (PMN). Upon PMN cell death is liberated from the phagolysosome and depletes arginine in the microenvironment leading to suppressed T cell and natural killer (NK) cell proliferation and cytokine secretion (By similarity). In group 2 innate lymphoid cells (ILC2s) promotes acute type 2 inflammation in the lung and is involved in optimal ILC2 proliferation but not survival. Plays a role in the immune response of alternatively activated or M2 macrophages in processes such as wound healing and tissue regeneration, immune defense against multicellular pathogens and parasites, and immune suppression and allergic inflammation; the regulatory outcome seems to be organ specific. In tumor-infiltrating dendritic cells (DCs) and myeloid-derived suppressor cells (MDSCs) plays a role in suppression of T cell-mediated antitumor immunity.

See full target information Arg1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of immunology (Baltimore, Md. : 1950) 206:2478-2488 PubMed33903172

2021

HE4 Overexpression by Ovarian Cancer Promotes a Suppressive Tumor Immune Microenvironment and Enhanced Tumor and Macrophage PD-L1 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Rachael B Rowswell-Turner,Rakesh K Singh,Anze Urh,Naohiro Yano,Kyu Kwang Kim,Negar Khazan,Ravina Pandita,Umayal Sivagnanalingam,Virginia Hovanesian,Nicole E James,Jennifer R Ribeiro,Sindhuja Kadambi,David C Linehan,Richard G Moore

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rat Arginase1 ELISA Kit

Sandwich ELISA - Rat Arginase1 ELISA Kit (AB231923)

Sandwich ELISA - Rat Arginase1 ELISA Kit (AB231923)

Sandwich ELISA - Rat Arginase1 ELISA Kit (AB231923)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (1)

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