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AB263883

Rat AST ELISA Kit (Aspartate Aminotransferase)

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(18 Publications)

Rat AST ELISA Kit (Aspartate Aminotransferase) is a single-wash 90-min Simplestep used to quantify Rat AST (Aspartate Aminotransferase) with a sensitivity of 42.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 15 citations

View Alternative Names

cAspAT, Glutamate oxaloacetate transaminase 1, Transaminase A, cCAT, Got1

3 Images
Sandwich ELISA - Rat AST ELISA Kit (Aspartate Aminotransferase) (AB263883)
  • sELISA

Supplier Data

Sandwich ELISA - Rat AST ELISA Kit (Aspartate Aminotransferase) (AB263883)

Interpolated concentrations of native AST in rat serum and plasma samples.

The concentrations of AST were measured in duplicates, interpolated from the AST standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 20, plasma (citrate) 1 : 20, plasma (EDTA) 1 : 20, and plasma (heparin) 1 : 40. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AST concentration was determined to be 148 ng/mL in serum, 143 ng/mL in plasma (citrate), 127 ng/mL in plasma (EDTA), and 174 ng/mL in plasma (heparin).

Sandwich ELISA - Rat AST ELISA Kit (Aspartate Aminotransferase) (AB263883)
  • sELISA

Supplier Data

Sandwich ELISA - Rat AST ELISA Kit (Aspartate Aminotransferase) (AB263883)

Interpolated concentrations of native AST in rat liver extract based on a 6,250 ng/mL extract load.

The concentrations of AST were measured in duplicate and interpolated from the AST standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AST concentration was determined to be 5,247 pg/mL in rat liver extract.

Sandwich ELISA - Rat AST ELISA Kit (Aspartate Aminotransferase) (AB263883)
  • sELISA

Supplier Data

Sandwich ELISA - Rat AST ELISA Kit (Aspartate Aminotransferase) (AB263883)

Example of rat AST standard curve in Sample Diluent NS.

The AST standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 42.4 pg/mL

Range

125 - 8000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Rat AST ELISA Kit (Aspartate Aminotransferase) (ab263883) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of AST (Aspartate Aminotransferase) protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat AST (Aspartate Aminotransferase) with 42.4 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Cytoplasmic aspartate aminotransaminase (AST), is a key enzyme in the regulation of glutamate levels. It catalyzes the reversible transfer of an a-amino group between aspartate and glutamate. Given its central role in amino acid metabolism, it plays a critical role in many biological functions. In a clinical context, AST levels in the serum are used as a marker for liver health. This kit targets the full-length protein.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" } ]
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Recovery

[ { "sample": "Serum", "range": "92 - 110 %", "average": "= 98" }, { "sample": "EDTA Plasma", "range": "98 - 107 %", "average": "= 102" }, { "sample": "Heparin Plasma", "range": "105 - 118 %", "average": "= 112" }, { "sample": "Citrate plasma", "range": "106 - 116 %", "average": "= 112" }, { "sample": "Tissue Extracts", "range": "99 - 105 %", "average": "= 101" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aspartate Aminotransferase (AST) also known as aspartate transaminase or aspartate transferase is an enzyme important for amino acid metabolism. Mechanically it catalyzes the reversible transfer of an amino group from aspartate to α-ketoglutarate forming oxaloacetate and glutamate. AST has a molecular mass of approximately 92 kDa and is expressed in various tissues with high levels found in liver heart muscle and kidneys. Its presence in these tissues highlights its importance in cellular metabolic processes.
Biological function summary

AST facilitates the interconversion between aspartate and oxaloacetate playing a role in the amino acid and urea cycles. Though not part of a large complex AST works closely with similar enzymes such as alanine aminotransferase (ALT) to maintain amino acid balance and support energy production. This enzymatic activity is important in nitrogen metabolism and glutamate use which are necessary for synthesizing other essential molecules within the cell.

Pathways

AST participates in the citric acid cycle and malate-aspartate shuttle enabling efficient energy production and NADH transport. The enzyme assists in converting oxaloacetate a pivotal intermediate in the citric acid cycle to keep the cycle active ensuring efficient cellular respiration. Additionally in the malate-aspartate shuttle AST works alongside malate dehydrogenase to facilitate the transfer of reducing equivalents across the mitochondrial membrane which is essential for ATP generation.

Elevated AST levels often indicate liver damage such as in hepatitis or cirrhosis reflecting its significant tissue expression. The enzyme is also linked to myocardial infarction as damaged heart muscle releases AST into the bloodstream. In these conditions AST serves as a biomarker for tissue damage often in conjunction with ALT levels allowing clinicians to assess the extent of liver or cardiac injury.
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Product protocols

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Target data

Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.
See full target information Got1
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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:18460 PubMed40425777

2025

MOTS-c mimics exercise to combat diabetic liver fibrosis by targeting Keap1-Nrf2-Smad2/3.

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Feilong Chen,Zhiyu Li,Tutu Wang,Yu Fu,Lei Lyu,Chengyuan Xing,Shunchang Li, Li

Molecular medicine reports 30: PubMed39219289

2024

inhibits hepatic fibrosis and hepatic stellate cell activation by targeting the PI3K/Akt/mTOR pathway.

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Zhiheng Dong,Haibin Guan,Lu Wang,Lijuan Liang,Yifan Zang,Lan Wu,Lidao Bao

Nutrients 16: PubMed38931176

2024

The Metabolic and Endocrine Effects of a 12-Week Allulose-Rich Diet.

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Kevin B Cayabyab,Marley J Shin,Micah S Heimuli,Iris J Kim,Dominic P D'Agostino,Richard J Johnson,Andrew P Koutnik,Nick Bellissimo,David M Diamond,Nicholas G Norwitz,Juan A Arroyo,Paul R Reynolds,Benjamin T Bikman

Reports of biochemistry & molecular biology 12:403-414 PubMed38618269

2024

Melatonin Mitigates the Progression of Chemically Induced Hepatocellular Carcinoma in Rats via Targeting Wnt/Β-Catenin Pathway, and Small Noncoding miR-let-7b.

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Nesma Mohammed Bahaa Eldeen,Moataz Maher Kamel,Abbas Mohamed,Samaa Samir Kamar,Laila Rashed,Asmaa Mohammed ShamsEldeen

Cells 13: PubMed38534382

2024

Tofogliflozin Delays Portal Hypertension and Hepatic Fibrosis by Inhibiting Sinusoidal Capillarization in Cirrhotic Rats.

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Shohei Asada,Kosuke Kaji,Norihisa Nishimura,Aritoshi Koizumi,Takuya Matsuda,Misako Tanaka,Nobuyuki Yorioka,Shinya Sato,Koh Kitagawa,Tadashi Namisaki,Takemi Akahane,Hitoshi Yoshiji

Open veterinary journal 13:1718-1728 PubMed38292716

2024

The role of hesperidin as a cardioprotective strategy against doxorubicin-induced cardiotoxicity: The antioxidant, anti-inflammatory, antiapoptotic, and cytoprotective potentials.

Applications

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Species

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Fawiziah Khalaf Alharbi,Zafer S Alshehri,Faez F Alshehri,Sharif Alhajlah,Hesham A Khalifa,Naief Dahran,Wael A M Ghonimi

Chemical biology & drug design 103:e14386 PubMed37923393

2023

Protective effects of baicalin on diethyl nitrosamine-induced liver cirrhosis by suppressing oxidative stress and inflammation.

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Species

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Hui Wang,Yufei Chang,Xiao Liu,Linan Liu,Mingxi Hua,Ang Li

International heart journal 64:935-944 PubMed37778997

2023

Exosomes from Adipose-Derived Mesenchymal Stem Cells Protect Against Cyclophosphamide-Induced Cardiotoxicity in Rats.

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Species

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Jianhua Xiong,Binjun Ding,Wei Zhu,Lanlan Xu,Songping Yu

Scientific reports 13:16424 PubMed37777583

2023

Melatonin loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles reduce inflammation, inhibit apoptosis and protect rat's liver from the hazardous effects of CCL4.

Applications

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Species

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Alyaa Farid,Valina Michael,Gehan Safwat

International journal of molecular sciences 24: PubMed37373368

2023

Effects of Exercise Preconditioning on Doxorubicin-Induced Liver and Kidney Toxicity in Male and Female Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Franccesco P Boeno,Jay Patel,Ryan N Montalvo,Stephanie S Lapierre-Nguyen,Claire M Schreiber,Ashley J Smuder
View all publications
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