Rat AST ELISA Kit (Aspartate Aminotransferase) is a single-wash 90-min Simplestep used to quantify Rat AST (Aspartate Aminotransferase) with a sensitivity of 42.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 15 citations
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- Reports of biochemistry & molecular biology 12:403-4142024Melatonin Mitigates the Progression of Chemically Induced Hepatocellular Carcinoma in Rats via Targeting Wnt/Β-Catenin Pathway, and Small Noncoding miR-let-7b.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNesma Mohammed Bahaa Eldeen,Moataz Maher Kamel,Abbas Mohamed,Samaa Samir Kamar,Laila Rashed,Asmaa Mohammed ShamsEldeenPubMed 38618269
- Chemical biology & drug design 103:e143862023Protective effects of baicalin on diethyl nitrosamine-induced liver cirrhosis by suppressing oxidative stress and inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui Wang,Yufei Chang,Xiao Liu,Linan Liu,Mingxi Hua,Ang LiPubMed 37923393
- International heart journal 64:935-9442023Exosomes from Adipose-Derived Mesenchymal Stem Cells Protect Against Cyclophosphamide-Induced Cardiotoxicity in Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianhua Xiong,Binjun Ding,Wei Zhu,Lanlan Xu,Songping YuPubMed 37778997
- Scientific reports 13:164242023Melatonin loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles reduce inflammation, inhibit apoptosis and protect rat's liver from the hazardous effects of CCL4.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlyaa Farid,Valina Michael,Gehan SafwatPubMed 37777583
- International journal of molecular sciences 24:2023Effects of Exercise Preconditioning on Doxorubicin-Induced Liver and Kidney Toxicity in Male and Female Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFranccesco P Boeno,Jay Patel,Ryan N Montalvo,Stephanie S Lapierre-Nguyen,Claire M Schreiber,Ashley J SmuderPubMed 37373368
- eLife 12:2023Gene and protein expression and metabolic flux analysis reveals metabolic scaling in liver ex vivo and in vivo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNgozi D Akingbesote,Brooks P Leitner,Daniel G Jovin,Reina Desrouleaux,Dennis Owusu,Wanling Zhu,Zongyu Li,Michael N Pollak,Rachel J PerryPubMed 37219930
- Life (Basel, Switzerland) 12:2022Ameliorative Effect of D-Carvone against Hepatic Ischemia-Reperfusion-Induced Injury in Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaged E Mohamed,Nancy S YounisPubMed 36294936
- Antibiotics (Basel, Switzerland) 11:2022Anti-Biofilm and Antibacterial Activities of R. Br Secondary Metabolites: In Silico, In Vitro, and In Vivo Approaches.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNashwah G M Attallah,Omnia Momtaz Al-Fakhrany,Engy Elekhnawy,Ismail A Hussein,Moataz A Shaldam,Najla Altwaijry,Moneerah J Alqahtani,Walaa A NegmPubMed 35892383
- Annals of translational medicine 10:3502022Sevoflurane attenuates hepatic ischemia reperfusion injury by the miR-122/Nrf2 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKai Zhang,Xia Xu,Lihong HuPubMed 35433991
- Journal of cellular and molecular medicine 25:9983-99942021Dexmedetomidine alleviates hepatic ischaemia-reperfusion injury via the PI3K/AKT/Nrf2-NLRP3 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Wu,Gaolin Qiu,Hainie Zhang,Leilei Zhu,Gao Cheng,Yiqiao Wang,Yuanhai Li,Weiwei WuPubMed 34664412
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 125 - 8000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 42.4 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.
Alternative names
cAspAT, Glutamate oxaloacetate transaminase 1, Transaminase A, cCAT, Got1
What's included?
Recommended products
Rat AST ELISA Kit (Aspartate Aminotransferase) is a single-wash 90-min Simplestep used to quantify Rat AST (Aspartate Aminotransferase) with a sensitivity of 42.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 15 citations
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 125 - 8000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 42.4 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 2.5 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 2.2 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 98 | Range 92 - 110 % |
Sample type EDTA Plasma | Average % = 102 | Range 98 - 107 % |
Sample type Heparin Plasma | Average % = 112 | Range 105 - 118 % |
Sample type Citrate plasma | Average % = 112 | Range 106 - 116 % |
Sample type Tissue Extracts | Average % = 101 | Range 99 - 105 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Rat AST ELISA Kit (Aspartate Aminotransferase) (ab263883) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of AST (Aspartate Aminotransferase) protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat AST (Aspartate Aminotransferase) with 42.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Cytoplasmic aspartate aminotransaminase (AST), is a key enzyme in the regulation of glutamate levels. It catalyzes the reversible transfer of an a-amino group between aspartate and glutamate. Given its central role in amino acid metabolism, it plays a critical role in many biological functions. In a clinical context, AST levels in the serum are used as a marker for liver health. This kit targets the full-length protein.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Aspartate Aminotransferase (AST) also known as aspartate transaminase or aspartate transferase is an enzyme important for amino acid metabolism. Mechanically it catalyzes the reversible transfer of an amino group from aspartate to α-ketoglutarate forming oxaloacetate and glutamate. AST has a molecular mass of approximately 92 kDa and is expressed in various tissues with high levels found in liver heart muscle and kidneys. Its presence in these tissues highlights its importance in cellular metabolic processes.
- Biological function summary
AST facilitates the interconversion between aspartate and oxaloacetate playing a role in the amino acid and urea cycles. Though not part of a large complex AST works closely with similar enzymes such as alanine aminotransferase (ALT) to maintain amino acid balance and support energy production. This enzymatic activity is important in nitrogen metabolism and glutamate use which are necessary for synthesizing other essential molecules within the cell.
- Pathways
AST participates in the citric acid cycle and malate-aspartate shuttle enabling efficient energy production and NADH transport. The enzyme assists in converting oxaloacetate a pivotal intermediate in the citric acid cycle to keep the cycle active ensuring efficient cellular respiration. Additionally in the malate-aspartate shuttle AST works alongside malate dehydrogenase to facilitate the transfer of reducing equivalents across the mitochondrial membrane which is essential for ATP generation.
- Associated diseases and disorders
Elevated AST levels often indicate liver damage such as in hepatitis or cirrhosis reflecting its significant tissue expression. The enzyme is also linked to myocardial infarction as damaged heart muscle releases AST into the bloodstream. In these conditions AST serves as a biomarker for tissue damage often in conjunction with ALT levels allowing clinicians to assess the extent of liver or cardiac injury.
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3 product images
Sandwich ELISA - Rat AST ELISA Kit (Aspartate Aminotransferase) (ab263883)
Example of rat AST standard curve in Sample Diluent NS.
The AST standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Rat AST ELISA Kit (Aspartate Aminotransferase) (ab263883)
Interpolated concentrations of native AST in rat serum and plasma samples.
The concentrations of AST were measured in duplicates, interpolated from the AST standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:20, plasma (citrate) 1:20, plasma (EDTA) 1:20, and plasma (heparin) 1:40. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AST concentration was determined to be 148 ng/mL in serum, 143 ng/mL in plasma (citrate), 127 ng/mL in plasma (EDTA), and 174 ng/mL in plasma (heparin).
Sandwich ELISA - Rat AST ELISA Kit (Aspartate Aminotransferase) (ab263883)
Interpolated concentrations of native AST in rat liver extract based on a 6,250 ng/mL extract load.
The concentrations of AST were measured in duplicate and interpolated from the AST standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AST concentration was determined to be 5,247 pg/mL in rat liver extract.
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