AB264619

Rat Beta-2-Microglobulin ELISA Kit

Recombinant
SimpleStep
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Rat Beta-2-Microglobulin ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat Beta-2-Microglobulin with a sensitivity of 268 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Beta-2-microglobulin, B2m

4 Images

Sandwich ELISA - Rat Beta-2-Microglobulin ELISA Kit (AB264619)

sELISA

Supplier Data

Sandwich ELISA - Rat Beta-2-Microglobulin ELISA Kit (AB264619)

Representative image of Rat Beta-2-Microglobulin ELISA Kit (ab264619)

Components shown from left to right :

- Sample diluent NS

- Denaturant

- 10X Rat Beta-2-Microglobulin Capture Antibody

- 10X Rat Beta-2-Microglobulin Detector Antibody

- Rat Beta-2-Microglobulin Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Stop Solution

- TMB solution

- 10X Wash Buffer PT (ab206977)

- Antibody Diluent 5BR

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

sELISA

Supplier Data

Sandwich ELISA - Rat Beta-2-Microglobulin ELISA Kit (AB264619)

Example of rat Beta-2-Microglobulin standard curve in Sample Diluent NS + Enhancer.

The Beta-2-Microglobulin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Rat Beta-2-Microglobulin ELISA Kit (AB264619)

Interpolated concentrations of native Beta-2-Microglobulin in rat urine and cell culture supernatant samples.

The concentrations of Beta-2-Microglobulin were measured in duplicates, interpolated from the Beta-2-Microglobulin standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 1 : 150 and lung cell culture supernatant 1 : 100. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Beta-2-Microglobulin concentration was determined to be 491.5 ng/mL in neat rat urine and 434.3 ng/mL in neat rat lung cell culture supernatant.

sELISA

Supplier Data

Sandwich ELISA - Rat Beta-2-Microglobulin ELISA Kit (AB264619)

Interpolated concentrations of native Beta-2-Microglobulin in rat serum and plasma samples.

The concentrations of Beta-2-Microglobulin were measured in duplicates, interpolated from the Beta-2-Microglobulin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 3,000, plasma (citrate) 1 : 1,500, plasma (heparin) 1 : 2,250, and plasma (EDTA) 1 : 2,250. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Beta-2-Microglobulin concentration was determined to be 12.23 μg/mL in neat serum, 7.025 μg/mL in neat plasma (citrate), 8.710 μg/mL in neat plasma (heparin), and 7.155 μg/mL in neat plasma (EDTA).

Key facts

Detection method

Colorimetric

Sample types

Urine, Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 268 pg/mL

Range

439 - 5000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Rat Beta-2-Microglobulin ELISA Kit (ab264619) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Beta-2-Microglobulin protein in cell culture media, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat Beta-2-Microglobulin with 268 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Beta-2-Microglobulin is the light chain of the class I major histocompatibility complex (MHC); it forms heterodimers with classical (HLA-A, -B, -C) and non-classical (HLA-E, -F, -G) class I MHC heavy chains and with class I non-MHC heavy chains (CD1). Beta-2-Microglobulin is involved in the presentation of peptide antigens to the immune system. Beta-2-Microglobulin can dissociate from the MHC and circulate in complex with alpha 2-Macroglobulin.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.1" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "110 - 123 %", "average": "= 115" }, { "sample": "Serum", "range": "111 - 118 %", "average": "= 115" }, { "sample": "EDTA Plasma", "range": "117 - 119 %", "average": "= 118" }, { "sample": "Urine", "range": "116 - 123 %", "average": "= 119" }, { "sample": "Cell culture media", "range": "93 - 104 %", "average": "= 99" }, { "sample": "Heparin Plasma", "range": "106 - 114 %", "average": "= 111" }, { "sample": "Citrate plasma", "range": "107 - 111 %", "average": "= 109" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab264619-1x96Tests", "assetComponentDetails": [ { "size":"1 x 500 µL", "name":"Denaturant", "number":"AB264619-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB264619-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB264619-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB264619-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB264619-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat Beta-2-Microglobulin Detector Antibody", "number":"AB264619-CMP10", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB264619-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB264619-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB264619-CMP05", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB264619-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rat Beta-2-Microglobulin Lyophilized Recombinant Protein", "number":"AB264619-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat Beta-2-Microglobulin Capture Antibody", "number":"AB264619-CMP09", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta-2-Microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC I) and plays an important role in presenting peptides to the immune system. B2M weighs approximately 11.8 kDa and is found abundantly in all nucleated cells. It has alternate names such as B2 microglobulin or beta-2-microglobulin. This protein is present in the cell membrane as a part of the MHC I which is important for immune surveillance. Additionally B2M is detectable in various biological fluids including serum and its levels can reflect physiological and pathological states.

Biological function summary

Beta-2-microglobulin is important for the stability and transport of MHC class I molecules to the cell surface. As part of the MHC class I complex B2M assists in binding peptides allowing immune cells to identify and target pathogen-infected cells. Without B2M the MHC class I molecules are not properly expressed on the cell surface disrupting immune recognition. In laboratory settings researchers often use anti-beta-2-microglobulin antibodies to investigate its role in MHC class I function.

Pathways

Beta-2-microglobulin interacts significantly with the immune system most notably in the antigen processing and presentation pathway. It works alongside proteins such as the heavy chain of MHC class I. B2M is important in the pathway that involves the transport of antigens to the endoplasmic reticulum where they are loaded onto MHC class I molecules for inspection by cytotoxic T cells. Another related pathway is the tapasin-mediated processing of antigen peptides highlighting the indispensable role of B2M in immune response regulation.

Beta-2-microglobulin is associated with conditions such as beta-2-microglobulin amyloidosis and certain lymphoproliferative disorders. Elevated levels of B2M in serum serve as a marker for diseases like multiple myeloma where the protein level correlates with disease severity. B2M-related amyloidosis frequently occurs in patients undergoing long-term dialysis where amyloid deposits accumulate in tissues. Linking B2M to immune system dysfunction studies have shown interactions with other proteins including components of the immune system like HLA-A and HLA-B highlighting B2M's relevance in diagnosing and understanding these conditions.

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Product protocols

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Target data

Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.

See full target information B2m

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rat Beta-2-Microglobulin ELISA Kit

Sandwich ELISA - Rat Beta-2-Microglobulin ELISA Kit (AB264619)

Sandwich ELISA - Rat Beta-2-Microglobulin ELISA Kit (AB264619)

Sandwich ELISA - Rat Beta-2-Microglobulin ELISA Kit (AB264619)

Sandwich ELISA - Rat Beta-2-Microglobulin ELISA Kit (AB264619)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Assay Kits

Secondary Antibodies

Proteins & Peptides

Product promise