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AB100757

Rat betaNGF ELISA Kit

4

(1 Review)

|

(7 Publications)

Rat betaNGF ELISA Kit is a Sandwich ELISA for the measurement of Rat betaNGF in Rat in Biofluids, Cell Culture Media samples.

View Alternative Names

Ngfb, Ngf, Beta-nerve growth factor, Beta-NGF

2 Images
Sandwich ELISA - Rat betaNGF ELISA Kit (AB100757)
  • sELISA

Supplier Data

Sandwich ELISA - Rat betaNGF ELISA Kit (AB100757)

Representative Standard Curve using ab100757.

Sandwich ELISA - Rat betaNGF ELISA Kit (AB100757)
  • sELISA

Supplier Data

Sandwich ELISA - Rat betaNGF ELISA Kit (AB100757)

Representative Standard Curve using ab100757.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 40 pg/mL

Range

0.049 - 100 ng/mL

Assay Platform

Microplate

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's betaNGF Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat betaNGF in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Rat betaNGF coated on a 96-well plate. Standards and samples are pipetted into the wells and betaNGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat betaNGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of betaNGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "123 - 142 %", "average": "= 132.1" }, { "sample": "Serum", "range": "102 - 121 %", "average": "= 111.7" }, { "sample": "Plasma", "range": "70 - 126 %", "average": "= 97.02" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100757-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100757-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100757-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"betaNGF Microplate (12 strips x 8 wells)", "number":"AB100757-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100757-CMP03", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB100757-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"betaNGF Standard Rat (recombinant) (lyophilized)", "number":"AB100757-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Rat beta-NGF", "number":"AB100757-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100757-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100757-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta nerve growth factor (beta-NGF) is a significant protein that functions in the development and maintenance of the nervous system. It is also known as NGF or NGFB and has a molecular mass of around 26 kDa. This protein is highly expressed in a wide range of tissues including the brain skin and various glands. Researchers often use assays like growth factor assays and NGF ELISA kits to measure beta-NGF levels given its importance in several physiological processes.
Biological function summary

Beta nerve growth factor plays an important role in the growth survival and differentiation of neurons. As a member of the neurotrophin family it binds to receptors like TrkA and p75NTR on the surface of nerve cells. This binding activity helps to promote neuronal health and function especially in the peripheral and central nervous systems. While beta-NGF traditionally acts independently it also interacts with other signaling molecules to trigger cascades that support cell survival and neural connections.

Pathways

Beta-NGF prominently participates in the MAPK/ERK and PI3K/AKT signaling pathways. These pathways are essential for transmitting signals from the cell surface to the DNA in the nucleus therefore regulating gene expression linked to cell growth and survival. In these contexts beta-NGF works closely with proteins like Akt and ERK enhancing neuron resilience and promoting restorative mechanisms across the nervous system.

Beta nerve growth factor has been connected to neurodegenerative diseases like Alzheimer's disease and chronic pain conditions. Alterations in beta-NGF expression or signaling can impact neuronal survival contributing to the progression of these disorders. In Alzheimer's disease for example changes in NGF interactions with proteins like amyloid-beta and tau may exacerbate neurodegeneration. Similarly in pain syndromes disruptions in beta-NGF signaling can influence pain perception through pathways involving other neurotrophic factors.
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Product protocols

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Target data

Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades to regulate neuronal proliferation, differentiation and survival (By similarity). The immature NGF precursor (proNGF) functions as a ligand for the heterodimeric receptor formed by SORCS2 and NGFR, and activates cellular signaling cascades that lead to inactivation of RAC1 and/or RAC2, reorganization of the actin cytoskeleton and neuronal growth cone collapse. In contrast to mature NGF, the precursor form (proNGF) promotes neuronal apoptosis (in vitro) (By similarity). Inhibits metalloproteinase-dependent proteolysis of platelet glycoprotein VI (By similarity). Binds lysophosphatidylinositol and lysophosphatidylserine between the two chains of the homodimer. The lipid-bound form promotes histamine relase from mast cells, contrary to the lipid-free form (By similarity).
See full target information Ngf
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

American journal of translational research 17:2197-2209 PubMed40226016

2025

Development of a predictive model for the relationship between serum pan-immunoinflammatory index levels and scar formation in facial burn patients.

Applications

Unspecified application

Species

Unspecified reactive species

Di Xi,Hailun Yu,Tong Yu

Journal of integrative neuroscience 21:79 PubMed35633160

2022

The influence of electronic acupuncture at a specific frequency in facilitating the passage of NGF through the blood-brain barrier and its effect on learning and memory in MCAO/R rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yibin Zhao,Xuqing Mao,Hao Wang,Lin Gan,Shanshan Zhang,Peng Gong,Xianming Lin

Neural regeneration research 16:871-877 PubMed33229722

2020

Functional recovery after peripheral nerve injury via sustained growth factor delivery from mineral-coated microparticles.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel J Hellenbrand,Clayton L Haldeman,Jae-Sung Lee,Angela G Gableman,Elena K Dai,Stephen D Ortmann,Jerrod C Gotchy,Kierra K Miller,Adrianna M Doucas,Nicole C Nowak,William L Murphy,Amgad S Hanna

Advanced healthcare materials 6: PubMed28218474

2017

Electrical Differentiation of Mesenchymal Stem Cells into Schwann-Cell-Like Phenotypes Using Inkjet-Printed Graphene Circuits.

Applications

Unspecified application

Species

Unspecified reactive species

Suprem R Das,Metin Uz,Shaowei Ding,Matthew T Lentner,John A Hondred,Allison A Cargill,Donald S Sakaguchi,Surya Mallapragada,Jonathan C Claussen

Journal of cellular physiology 231:1301-12 PubMed26516696

2015

Ex Vivo Assay of Electrical Stimulation to Rat Sciatic Nerves: Cell Behaviors and Growth Factor Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiyong Du,Olexandr Bondarenko,Dingkun Wang,Mahmoud Rouabhia,Ze Zhang

PloS one 10:e0133971 PubMed26230083

2015

Cardiac Dysregulation and Myocardial Injury in a 6-Hydroxydopamine-Induced Rat Model of Sympathetic Denervation.

Applications

Unspecified application

Species

Unspecified reactive species

Yue-Hua Jiang,Ping Jiang,Jin-Long Yang,Du-Fang Ma,Hai-Qing Lin,Wen-Ge Su,Zhen Wang,Xiao Li

American journal of physiology. Heart and circulat 301:H1070-9 PubMed21642505

2011

Contribution of nerve growth factor to upregulation of P2X₃ expression in DRG neurons of rats with femoral artery occlusion.

Applications

Unspecified application

Species

Unspecified reactive species

Jiahao Liu,Jialiu David Li,Jian Lu,Jihong Xing,Jianhua Li
View all publications
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Product promise

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