AB108815

Rat BNP 32 ELISA Kit

Name: Rat BNP 32 ELISA Kit (ab108815) | Abcam
Brand: Abcam Limited
SKU: AB108815
Price: 780 USD
Availability: InStock

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(12 Publications)

Rat BNP 32 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Rat BNP 32 in Rat in Biofluids, Cell/Tissue Extracts samples.

View Alternative Names

Natriuretic peptides B, Brain natriuretic factor prohormone, Gamma-brain natriuretic peptide, Iso-ANP, preproBNP, proBNP, Nppb

1 Images

Sandwich ELISA - Rat BNP 32 ELISA Kit (AB108815)

sELISA

Supplier Data

Sandwich ELISA - Rat BNP 32 ELISA Kit (AB108815)

Representative Standard Curve using ab108815.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum, Tissue, Cell Lysate

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 18 pg/mL

Range

0.031 - 2 ng/mL

Assay time

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Abcam's BNP 32 Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of BNP 32 in plasma, serum, tissue extract, and cell culture supernatants.

A BNP 32 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a BNP 32 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of BNP 32 captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

There is significant cross-reactivity between BNP32 and BNP45.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.7" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.9" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108815-1x96Tests", "assetComponentDetails": [ { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108815-CMP03", "productcode":"" }, { "size":"1 x 150 µL", "name":"40X Biotinylated Rat BNP 32 Antibody", "number":"AB108815-CMP09", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108815-CMP06", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108815-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"BNP 32 Microplate (12 x 8 well strips)", "number":"AB108815-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"BNP 32 Standard", "number":"AB108815-CMP05", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108815-CMP08", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB108815-CMP01", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108815-CMP07", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

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Product protocols

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Target data

Brain natriuretic peptide 45. Cardiac hormone that plays a key role in mediating cardio-renal homeostasis (PubMed : 2525380, PubMed : 9252368). May also function as a paracrine antifibrotic factor in the heart (By similarity). Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins that drive various biological responses (By similarity). Likely involved in regulating the extracellular fluid volume and maintaining the fluid-electrolyte balance through natriuresis, diuresis, kaluresis and chloruresis (PubMed : 2525380, PubMed : 9252368).

See full target information Nppb

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 11:1252942 PubMed37766966

2023

Tanshinone IIA inhibits cardiomyocyte pyroptosis through TLR4/NF-κB p65 pathway after acute myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Ruoning Chai,Zelin Ye,Wenjing Xue,Shuqing Shi,Yi Wei,Yuanhui Hu,Huaqin Wu

Journal of molecular medicine (Berlin, Germany) 101:265-278 PubMed36688960

2023

Inhibition of ERK/CREB signaling contributes to postoperative learning and memory dysfunction in neonatal rats.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Wang,Gang Ma,Jia Min,Jun Li,Weiran Shan,Zhiyi Zuo

Cells 11: PubMed35203391

2022

Sustained Elevated Circulating Activin A Impairs Global Longitudinal Strain in Pregnant Rats: A Potential Mechanism for Preeclampsia-Related Cardiac Dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Bhavisha A Bakrania,Ana C Palei,Umesh Bhattarai,Yingjie Chen,Joey P Granger,Sajid Shahul

The Tohoku journal of experimental medicine 254:221-231 PubMed34321385

2021

Long Non-Coding RNA SOX2 Overlapping Transcript Aggravates H9c2 Cell Injury via the miR-215-5p/ZEB2 Axis and Promotes Ischemic Heart Failure in a Rat Model.

Applications

Unspecified application

Species

Unspecified reactive species

Jiahong Tu,Liping Ma,Mingqing Zhang,Jie Zhang

Cells 10: PubMed34359915

2021

Piezo1 Mechanosensitive Ion Channel Mediates Stretch-Induced Nppb Expression in Adult Rat Cardiac Fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Meike C Ploeg,Chantal Munts,Frits W Prinzen,Neil A Turner,Marc van Bilsen,Frans A van Nieuwenhoven

Oxidative medicine and cellular longevity 2021:6643871 PubMed33728024

2021

Modulation of Mitochondrial Quality Control Processes by BGP-15 in Oxidative Stress Scenarios: From Cell Culture to Heart Failure.

Applications

Unspecified application

Species

Unspecified reactive species

Orsolya Horvath,Katalin Ordog,Kitti Bruszt,Nikoletta Kalman,Dominika Kovacs,Balazs Radnai,Ferenc Gallyas,Kalman Toth,Robert Halmosi,Laszlo Deres

Oxidative medicine and cellular longevity 2021:1250858 PubMed33564362

2021

BGP-15 Protects against Heart Failure by Enhanced Mitochondrial Biogenesis and Decreased Fibrotic Remodelling in Spontaneously Hypertensive Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Orsolya Horvath,Katalin Ordog,Kitti Bruszt,Laszlo Deres,Ferenc Gallyas,Balazs Sumegi,Kalman Toth,Robert Halmosi

Experimental and therapeutic medicine 18:779-785 PubMed31281454

2019

MicroRNA-146a improves sepsis-induced cardiomyopathy by regulating the TLR-4/NF-κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Xie,Lina Zhang,Xiaoyan Fan,Xiaoqing Dong,Zhe Zhang,Wenxing Fan

American journal of physiology. Heart and circulat 317:H87-H96 PubMed31050559

2019

Exercise training restores the myogenic response in skeletal muscle resistance arteries and corrects peripheral edema in rats with heart failure.

Applications

Unspecified application

Species

Unspecified reactive species

Suliana M Paula,Gisele K Couto,Milene T Fontes,Soraia K Costa,Carlos E Negrão,José G Mill,Luciana V Rossoni

Scientific reports 7:8588 PubMed28819266

2017

Amalaki rasayana, a traditional Indian drug enhances cardiac mitochondrial and contractile functions and improves cardiac function in rats with hypertrophy.

Applications

sELISA

Species

Unspecified reactive species

Vikas Kumar,Kumar A Aneesh,K Kshemada,Kumar G S Ajith,Raj S S Binil,Neha Deora,G Sanjay,A Jaleel,T S Muraleedharan,E M Anandan,R S Mony,M S Valiathan,Kumar T R Santhosh,C C Kartha

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rat BNP 32 ELISA Kit

Sandwich ELISA - Rat BNP 32 ELISA Kit (AB108815)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (12)

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