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Rat BNP 45 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Rat BNP 45 in Rat in Plasma, Serum, Cell Lysate, Tissue samples.


Images

Sandwich ELISA - Rat BNP 45 ELISA Kit (AB108816), expandable thumbnail
  • Sandwich ELISA - Rat BNP 45 ELISA Kit (AB108816), expandable thumbnail

Publications

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum, Cell Lysate, Tissue

Assay type

Sandwich (quantitative)

Reactive species

Rat

Range

15.625 - 1000 pg/mL

Sensitivity

= 7.9 ng/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Brain natriuretic peptide 45Cardiac hormone that plays a key role in mediating cardio-renal homeostasis (PubMed:2525380, PubMed:9252368). May also function as a paracrine antifibrotic factor in the heart (By similarity). Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins that drive various biological responses (By similarity). Likely involved in regulating the extracellular fluid volume and maintaining the fluid-electrolyte balance through natriuresis, diuresis, kaluresis and chloruresis (PubMed:2525380, PubMed:9252368).

Alternative names

What's included?

1 x 96 Tests
Components
100X Streptavidin-Peroxidase Conjugate
1 x 80 µL
10X Diluent N Concentrate
1 x 30 mL
20X Wash Buffer Concentrate
2 x 30 mL
70X Biotinylated Rat BNP 45 Antibody
1 x 90 µL
BNP 45 Microplate (12 x 8 well strips)
1 x 1 Unit
BNP 45 Standard (Lyophilized)
1 x 1 Vial
Chromogen Substrate
1 x 7 mL
Sealing Tapes
3 x 1 Unit
Stop Solution
1 x 11 mL

Recommended products

Rat BNP 45 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Rat BNP 45 in Rat in Plasma, Serum, Cell Lysate, Tissue samples.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum, Cell Lysate, Tissue

Assay type

Sandwich (quantitative)

Reactive species

Rat

Range

15.625 - 1000 pg/mL

Assay Platform

Microplate

Sensitivity

= 7.9 ng/mL

Precision

Intra assay

Sample

Overall

n

0

mean

-

SD

-

C.V.

5.5

Inter assay

Sample

Overall

n

0

mean

-

SD

-

C.V.

10.4

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

Notes

Abcam's BNP 45 rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of BNP 45 in plasma, serum, tissue extract, and cell culture supernatants.

A BNP 45 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a BNP 45 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of BNP 45 captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

There is significant cross-reactivity between BNP32 and BNP45.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Sandwich ELISA - Rat BNP 45 ELISA Kit (ab108816), expandable thumbnail

    Sandwich ELISA - Rat BNP 45 ELISA Kit (ab108816)

    Rat BNP45 measured in biological fluids showing quantity (pg) per mL of tested sample

  • Sandwich ELISA - Rat BNP 45 ELISA Kit (ab108816), expandable thumbnail

    Sandwich ELISA - Rat BNP 45 ELISA Kit (ab108816)

    Representative Standard Curve using ab108816

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com