Rat BNP 45 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Rat BNP 45 in Rat in Plasma, Serum, Cell Lysate, Tissue samples.
Colorimetric
Plasma, Serum, Cell Lysate, Tissue
Sandwich (quantitative)
Rat
15.625 - 1000 pg/mL
= 7.9 ng/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Brain natriuretic peptide 45Cardiac hormone that plays a key role in mediating cardio-renal homeostasis (PubMed:2525380, PubMed:9252368). May also function as a paracrine antifibrotic factor in the heart (By similarity). Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins that drive various biological responses (By similarity). Likely involved in regulating the extracellular fluid volume and maintaining the fluid-electrolyte balance through natriuresis, diuresis, kaluresis and chloruresis (PubMed:2525380, PubMed:9252368).
Natriuretic peptides B, Brain natriuretic factor prohormone, Gamma-brain natriuretic peptide, Iso-ANP, preproBNP, proBNP, Nppb
Rat BNP 45 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Rat BNP 45 in Rat in Plasma, Serum, Cell Lysate, Tissue samples.
Colorimetric
Plasma, Serum, Cell Lysate, Tissue
Sandwich (quantitative)
Rat
15.625 - 1000 pg/mL
Microplate
= 7.9 ng/mL
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. 5.5 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. 10.4 |
Blue Ice
-20°C
Multi
Please refer to protocols
Abcam's BNP 45 rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of BNP 45 in plasma, serum, tissue extract, and cell culture supernatants.
A BNP 45 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a BNP 45 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of BNP 45 captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
There is significant cross-reactivity between BNP32 and BNP45.
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Terms & Conditions.
Rat BNP45 measured in biological fluids showing quantity (pg) per mL of tested sample
Representative Standard Curve using ab108816
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