AB108827

Rat C Reactive Protein ELISA Kit (PTX1)

Name: Rat C Reactive Protein ELISA Kit (PTX1) (ab108827) | Abcam
Brand: Abcam Limited
SKU: AB108827
Price: 780 USD
Availability: InStock

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(21 Publications)

Rat C Reactive Protein ELISA Kit (PTX1) is a Sandwich ELISA for the measurement of Rat C Reactive Protein (PTX1) in Rat in Biofluids samples.

View Alternative Names

Ptx1, Crp, C-reactive protein

1 Images

Sandwich ELISA - Rat C Reactive Protein ELISA Kit (PTX1) (AB108827)

sELISA

Supplier Data

Sandwich ELISA - Rat C Reactive Protein ELISA Kit (PTX1) (AB108827)

Representative Standard Curve using ab108827.

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Serum

Reacts with

Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

>= 0.2 ng/mL

Range

0.391 - 25 ng/mL

Assay time

3h 30m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Abcam's C Reactive Protein (PTX1) Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of C Reactive Protein in urine, plasma and serum.

A C Reactive Protein specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a C Reactive Protein specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of C Reactive Protein captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.8" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.3" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108827-1x96Tests", "assetComponentDetails": [ { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108827-CMP06", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent M Concentrate", "number":"AB108827-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"C Reactive Protein Standard", "number":"AB108827-CMP03", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108827-CMP09", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108827-CMP04", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108827-CMP08", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Rat C Reactive Protein Antibody", "number":"AB108827-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"C Reactive Protein Microplate (12 x 8 well strips)", "number":"AB108827-CMP02", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108827-CMP07", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C-reactive protein (CRP) also known as CRP protein is an acute-phase protein with a molecular mass of approximately 25 kDa. It is primarily synthesized in the liver in response to cytokines. This protein appears in the blood where it binds to phosphocholine expressed on the surface of dead or dying cells. CRP is often used as a biomarker for inflammation in the body and its levels in human and canine (anti CRP used) blood samples are measured using assays like the CRP ELISA test or CRP ELISA kit.

Biological function summary

CRP plays a significant role in the innate immune system by recognizing foreign pathogens and damaged cells. It does not form part of a larger complex but itself can bind to specific ligands initiating the complement cascade. This activation enhances phagocytosis by macrophages contributing to the clearance of necrotic and apoptotic cells. The CRP protein therefore acts as a fast responder in the presence of inflammation making it a useful indicator for monitoring inflammatory diseases.

Pathways

CRP operates predominantly within the acute-phase response pathway. Its production is upregulated by cytokines such as IL-6 which connects it to inflammation-signaling pathways. CRP also interacts with the complement system bridging innate and adaptive immunity. Besides CRP could indirectly influence pathways involving other inflammatory proteins like serum amyloid A and haptoglobin creating a network of responses important for managing inflammation.

Elevated CRP levels are commonly associated with cardiovascular diseases and rheumatoid arthritis. In cardiovascular diseases CRP acts as a marker for atherosclerosis where the protein levels correlate with disease severity. It connects with other proteins involved in inflammatory processes such as fibrinogen to contribute to disease pathology. In rheumatoid arthritis CRP provides insights into disease activity helping in the assessment of treatment responses.

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Product protocols

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Target data

Displays several functions associated with host defense : it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells (By similarity).

See full target information Crp

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Publications (21)

Recent publications for all applications. Explore the full list and refine your search

Molecules (Basel, Switzerland) 28: PubMed37764336

2023

A Standardized Botanical Composition Mitigated Acute Inflammatory Lung Injury and Reduced Mortality through Extracellular HMGB1 Reduction.

Applications

Unspecified application

Species

Unspecified reactive species

Mesfin Yimam,Teresa Horm,Alexandria O'Neal,Ping Jiao,Mei Hong,Lidia Brownell,Qi Jia,Mosi Lin,Alex Gauthier,Jiaqi Wu,Kranti Venkat Mateti,Xiaojian Yang,Katelyn Dial,Sidorela Zefi,Lin L Mantell

BMC endocrine disorders 22:293 PubMed36434695

2022

Novel sedentary cage induced sedentariness in rats: evidence from relevant biomarkers.

Applications

Unspecified application

Species

Unspecified reactive species

Quadri K Alabi,Rufus O Akomolafe

Oxidative medicine and cellular longevity 2022:5394303 PubMed35154566

2022

Serum Indicators of Oxidative Damage from Embedded Metal Fragments in a Rat Model.

Applications

Unspecified application

Species

Unspecified reactive species

John F Kalinich,Vernieda B Vergara,Jessica F Hoffman

TheScientificWorldJournal 2021:1393372 PubMed34887703

2021

Treadmill Exercise Training Ameliorates Functional and Structural Age-Associated Kidney Changes in Male Albino Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Heba R Salem,Manar A Faried

European journal of pharmacology 908:174309 PubMed34252442

2021

1-Methyl tryptophan, an indoleamine 2,3-dioxygenase inhibitor, attenuates cardiac and hepatic dysfunction in rats with biliary cirrhosis.

Applications

Unspecified application

Species

Unspecified reactive species

Sevda Shayesteh,Gilles J Guillemin,Amir Rashidian,Hedyeh Faghir-Ghanesefat,Ali R Mani,Seyed Mohammad Tavangar,Ahmad R Dehpour

Annals of palliative medicine 10:5299-5309 PubMed34107698

2021

Intermittent fasting therapy promotes insulin sensitivity by inhibiting NLRP3 inflammasome in rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Bing-Jun Liang,Sheng-Rong Liao,Wei-Xuan Huang,Chao Huang,Hao Sheng Liu,Wei-Zeng Shen

Saudi journal of biological sciences 28:3333-3342 PubMed34121870

2021

leaf extracts produce anti-obesity, hypoglycemic, anti-hyperlipidemic, and hepatoprotective effects on high-fat diet fed rats.

Applications

Unspecified application

Species

Unspecified reactive species

Dalal A Alkhudhayri,Magdi A Osman,Ghedeir M Alshammari,Salah A Al Maiman,Mohammed Abdo Yahya

Pharmaceutics 13: PubMed33670981

2021

6-Gingerol, a Bioactive Compound of Ginger Attenuates Renal Damage in Streptozotocin-Induced Diabetic Rats by Regulating the Oxidative Stress and Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Saleh A Almatroodi,Abdullah M Alnuqaydan,Ali Yousif Babiker,Mashael Abdullah Almogbel,Amjad Ali Khan,Arshad Husain Rahmani

Drug delivery and translational research : PubMed32441012

2020

Amniotic membrane mesenchymal stem cells labeled by iron oxide nanoparticles exert cardioprotective effects against isoproterenol (ISO)-induced myocardial damage by targeting inflammatory MAPK/NF-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Maryam Naseroleslami,Nahid Aboutaleb,Behnaz Mokhtari

BMC complementary medicine and therapies 20:33 PubMed32024512

2020

Opuntia cladode powders inhibit adipogenesis in 3 T3-F442A adipocytes and a high-fat-diet rat model by modifying metabolic parameters and favouring faecal fat excretion.

Applications

Unspecified application

Species

Unspecified reactive species

Cécile Héliès-Toussaint,Edwin Fouché,Nathalie Naud,Florence Blas-Y-Estrada,Maria Del Socorro Santos-Diaz,Anne Nègre-Salvayre,Ana Paulina Barba de la Rosa,Françoise Guéraud

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rat C Reactive Protein ELISA Kit (PTX1)

Sandwich ELISA - Rat C Reactive Protein ELISA Kit (PTX1) (AB108827)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Assay Kits

Assay Kits

Secondary Antibodies

Reactivity data

Product details

Precision

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (21)

Product promise