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AB289835

Rat Calcitonin ELISA Kit

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Rat Calcitonin ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Rat Calcitonin in Rat in Biofluids samples.

View Alternative Names

Calc, Calca, Calcitonin

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Sandwich ELISA - Rat Calcitonin ELISA Kit (AB289835)
  • sELISA

Supplier Data

Sandwich ELISA - Rat Calcitonin ELISA Kit (AB289835)

Typical Standard Curve using ab289835. A new standard curve should be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum, Other biological fluids

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 9.38 pg/mL

Range

15.63 - 1000 pg/mL

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This Rat Calcitonin ELISA ab289835 (previously known as #E4745) kit is an in vitro ELISA for the quantitative measurement of rat Calcitonin through sandwhich ELISA. This assay employs an antibody specific for rat Calcitonin coated on a 96-well plate.
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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.76" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.03" } ]
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What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab289835-96Test", "assetComponentDetails": [ { "size":"4 x 1 Unit", "name":"Plate Sealer", "number":"AB289835-CMP05" }, { "size":"1 x 10 mL", "name":"Stop Solution", "number":"AB289835-CMP07" }, { "size":"2 x 1 Vial", "name":"Standard", "number":"AB289835-CMP03" }, { "size":"1 x 30 mL", "name":"25X Wash buffer", "number":"AB289835-CMP11" }, { "size":"1 x 14 mL", "name":"Biotinylated Detection Antibody Diluent", "number":"AB289835-CMP02" }, { "size":"1 x 120 µL", "name":"HRP Conjugate (100x)", "number":"AB289835-CMP08" }, { "size":"1 x 10 mL", "name":"Substrate Reagent", "number":"AB289835-CMP10" }, { "size":"1 x 14 mL", "name":"HRP Conjugate Diluent", "number":"AB289835-CMP09" }, { "size":"1 x 20 mL", "name":"Standard & Sample Diluent", "number":"AB289835-CMP06" }, { "size":"1 x 1 Unit", "name":"Micro ELISA Plate", "number":"AB289835-CMP04" }, { "size":"1 x 120 µL", "name":"Biotinylated Detection Antibody (100x)", "number":"AB289835-CMP01" } ] }, "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab289835-96Test", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"25X Wash buffer", "number":"AB289835-CMP11", "productcode":"" }, { "size":"1 x 10 mL", "name":"Substrate Reagent", "number":"AB289835-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"Stop Solution", "number":"AB289835-CMP07", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Plate Sealer", "number":"AB289835-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Micro ELISA Plate", "number":"AB289835-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Standard", "number":"AB289835-CMP03", "productcode":"" }, { "size":"1 x 120 µL", "name":"HRP Conjugate (100x)", "number":"AB289835-CMP08", "productcode":"" }, { "size":"1 x 14 mL", "name":"HRP Conjugate Diluent", "number":"AB289835-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"Standard & Sample Diluent", "number":"AB289835-CMP06", "productcode":"" }, { "size":"1 x 120 µL", "name":"Biotinylated Detection Antibody (100x)", "number":"AB289835-CMP01", "productcode":"" }, { "size":"1 x 14 mL", "name":"Biotinylated Detection Antibody Diluent", "number":"AB289835-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calcitonin also known as thyrocalcitonin is a peptide hormone with a molecular mass of about 3.4 kDa. It consists of 32 amino acids and is primarily synthesized in parafollicular cells or C cells of the thyroid gland. This hormone regulates calcium and phosphate homeostasis in the body. It reduces blood calcium levels by inhibiting osteoclast activity in the bones and decreasing reabsorption in the kidneys leading to more calcium excretion. Calcitonin also interacts with its receptor a G protein-coupled receptor found in tissues like bone kidney and brain.
Biological function summary

This hormone plays a significant role in maintaining calcium levels by balancing the actions of parathyroid hormone (PTH). Calcitonin works independently but interacts with the broader system managing bone remodeling through an intricate network. The presence of calcitonin in specialized receptor complexes allows it to exert its effects efficiently in target tissues making it an important player in calcium and phosphorus metabolism.

Pathways

Calcitonin acts within the calcium signaling pathway and the bone resorption pathway. Through these pathways calcitonin works alongside other proteins such as parathyroid hormone (PTH) and calcitonin gene-related peptide (CGRP) maintaining skeletal integrity. It modulates the activity of osteoclasts ensuring that bone resorption activities are counterbalanced by bone formation and influences intracellular calcium concentration by binding to its receptor on target cells.

Calcitonin is linked to conditions such as osteoporosis and medullary thyroid carcinoma. Osteoporosis characterized by decreased bone density and increased fracture risk involves dysregulated calcium and phosphate balance. Proteins like estrogen and growth factors along with calcitonin influence bone health. Medullary thyroid carcinoma a type of thyroid cancer arising from C cells affects calcitonin levels with this hormone serving as a biomarker for diagnosing and monitoring treatment effectiveness. Calcitonin's connection with these diseases highlights its diagnostic and therapeutic potential.
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Product protocols

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Target data

Calcitonin is a peptide hormone that causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones. Calcitonin function is mediated by the calcitonin receptor/CALCR and the CALCR-RAMP2 (AMYR2) receptor complex (By similarity).
See full target information Calca
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websiteProtocolBooklet
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