Skip to main content

Rat Cardiac Troponin I ELISA Kit ab246529 is a single-wash 90-min SimpleStep ELISA used to quantify Rat Cardiac Troponin I with a sensitivity of 7.7 pg/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.

Easy-to-use, rapid, sensitive Rat Cardiac Troponin I sandwich ELISA Kit:
- Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format
- Colorimetric assay - 450nm readout; works on any plate reader
- Looking for more flexibility? We also offer antibody pairs ready for conjugation


Images

Sandwich ELISA - Rat Cardiac Troponin I ELISA Kit (AB246529), expandable thumbnail
  • Sandwich ELISA - Rat Cardiac Troponin I ELISA Kit (AB246529), expandable thumbnail
  • Sandwich ELISA - Rat Cardiac Troponin I ELISA Kit (AB246529), expandable thumbnail

Publications

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Rat

Range

15.63 - 1250 pg/mL

Assay time

1h 30m

Sensitivity

= 7.7 pg/mL

Abcam Recommends

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

Alternative names

What's included?

1 x 96 Tests
Components
10X Rat Cardiac Troponin I Capture Antibody
1 x 600 µL
10X Rat Cardiac Troponin I Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
5X Cell Extraction Buffer PTR (ab193970)
1 x 10 mL
Antibody Diluent CPR2
1 x 6 mL
Plate Seals
1 x 1 Unit
Rat Cardiac Troponin I Lyophilized Recombinant Protein
2 x 1 Vial
Sample Diluent NS (ab193972)
1 x 12 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

Recommended products

Rat Cardiac Troponin I ELISA Kit ab246529 is a single-wash 90-min SimpleStep ELISA used to quantify Rat Cardiac Troponin I with a sensitivity of 7.7 pg/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.

Easy-to-use, rapid, sensitive Rat Cardiac Troponin I sandwich ELISA Kit:
- Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format
- Colorimetric assay - 450nm readout; works on any plate reader
- Looking for more flexibility? We also offer antibody pairs ready for conjugation

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Rat

Range

15.63 - 1250 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 7.7 pg/mL

Precision

Intra assay

Sample

Serum

n

5

mean

-

SD

-

C.V.

3.1

Inter assay

Sample

Serum

n

3

mean

-

SD

-

C.V.

5.5

Recovery

Sample specific recovery

Sample type

Serum

Average %

= 101

Range

89 - 121 %

Sample type

EDTA Plasma

Average %

= 94

Range

89 - 99 %

Sample type

Tissue Extracts

Average %

= 110

Range

99 - 121 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Rat Cardiac Troponin I ELISA Kit (ab246529) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cardiac Troponin I protein in serum, tissue extracts, and edta plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat Cardiac Troponin I with 7.7 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Sandwich ELISA - Rat Cardiac Troponin I ELISA Kit (ab246529), expandable thumbnail

    Sandwich ELISA - Rat Cardiac Troponin I ELISA Kit (ab246529)

    Example of rat Cardiac Troponin I standard curve in Sample Diluent NS.

    The Cardiac Troponin I standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Rat Cardiac Troponin I ELISA Kit (ab246529), expandable thumbnail

    Sandwich ELISA - Rat Cardiac Troponin I ELISA Kit (ab246529)

    Example of rat Cardiac Troponin I standard curve in 1X Cell Extraction Buffer PTR.

    The Cardiac Troponin I standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Rat Cardiac Troponin I ELISA Kit (ab246529), expandable thumbnail

    Sandwich ELISA - Rat Cardiac Troponin I ELISA Kit (ab246529)

    Interpolated concentrations of native Cardiac Troponin I in rat heart homogenate extract based on a 333.3 pg/mL extract load.

    The concentrations of Cardiac Troponin I were measured in duplicate and interpolated from the Cardiac Troponin I standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cardiac Troponin I concentration was determined to be 857.0 pg/mL in heart homogenate extract.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com