Rat Cardiac Troponin I ELISA Kit ab246529 is a single-wash 90-min SimpleStep ELISA used to quantify Rat Cardiac Troponin I with a sensitivity of 7.7 pg/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.
Easy-to-use, rapid, sensitive Rat Cardiac Troponin I sandwich ELISA Kit:
- Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format
- Colorimetric assay - 450nm readout; works on any plate reader
- Looking for more flexibility? We also offer antibody pairs ready for conjugation
Colorimetric
Tissue Extracts, Serum, EDTA Plasma
Sandwich (quantitative)
Rat
15.63 - 1250 pg/mL
1h 30m
= 7.7 pg/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Ctni, Tni, Tnni3, Ctni, Tni, Cardiac troponin I
Rat Cardiac Troponin I ELISA Kit ab246529 is a single-wash 90-min SimpleStep ELISA used to quantify Rat Cardiac Troponin I with a sensitivity of 7.7 pg/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.
Easy-to-use, rapid, sensitive Rat Cardiac Troponin I sandwich ELISA Kit:
- Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format
- Colorimetric assay - 450nm readout; works on any plate reader
- Looking for more flexibility? We also offer antibody pairs ready for conjugation
Colorimetric
Tissue Extracts, Serum, EDTA Plasma
Sandwich (quantitative)
Rat
15.63 - 1250 pg/mL
1h 30m
Pre-coated microplate (12 x 8 well strips)
= 7.7 pg/mL
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Serum | n 5 | mean - | SD - | C.V. 3.1 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 5.5 |
Sample type | Average % | Range |
---|---|---|
Sample type Serum | Average % = 101 | Range 89 - 121 % |
Sample type EDTA Plasma | Average % = 94 | Range 89 - 99 % |
Sample type Tissue Extracts | Average % = 110 | Range 99 - 121 % |
Blue Ice
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Rat Cardiac Troponin I ELISA Kit (ab246529) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cardiac Troponin I protein in serum, tissue extracts, and edta plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat Cardiac Troponin I with 7.7 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Example of rat Cardiac Troponin I standard curve in Sample Diluent NS.
The Cardiac Troponin I standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Example of rat Cardiac Troponin I standard curve in 1X Cell Extraction Buffer PTR.
The Cardiac Troponin I standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Interpolated concentrations of native Cardiac Troponin I in rat heart homogenate extract based on a 333.3 pg/mL extract load.
The concentrations of Cardiac Troponin I were measured in duplicate and interpolated from the Cardiac Troponin I standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cardiac Troponin I concentration was determined to be 857.0 pg/mL in heart homogenate extract.
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