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AB108819

Rat Ceruloplasmin ELISA Kit (CERP)

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Rat Ceruloplasmin ELISA Kit (CERP) is a Sandwich ELISA for the measurement of Rat Ceruloplasmin (CERP) in Rat in Biofluids, Cell Culture Media, Cell/Tissue Extracts samples.

View Alternative Names

Ceruloplasmin, Cuproxidase ceruloplasmin, Ferroxidase ceruloplasmin, Glutathione peroxidase ceruloplasmin, Glutathione-dependent peroxiredoxin ceruloplasmin, Cp

1 Images
Sandwich ELISA - Rat Ceruloplasmin ELISA Kit (CERP) (AB108819)
  • sELISA

Supplier Data

Sandwich ELISA - Rat Ceruloplasmin ELISA Kit (CERP) (AB108819)

Representative Standard Curve using ab108819.

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Cell culture supernatant, Serum, Cell Lysate

Reacts with

Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 74 ng/mL

Range

1 - 10 ng/mL

Assay time

4h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Ceruloplasmin (CERP) rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Ceruloplasmin in urine and cell culture supernatants.

A Ceruloplasmin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Ceruloplasmin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Ceruloplasmin captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.3" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.8" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108819-1x96Tests", "assetComponentDetails": [ { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108819-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Ceruloplasmin Microplate (12 x 8 well strips)", "number":"AB108819-CMP05", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Rat Ceruloplasmin Antibody", "number":"AB108819-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Ceruloplasmin Standard", "number":"AB108819-CMP06", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108819-CMP02", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108819-CMP07", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108819-CMP08", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108819-CMP09", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB108819-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

Multifunctional blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane. Copper ions provide a large number of enzymatic activites. Oxidizes highly toxic ferrous ions to the ferric state for further incorporation onto apo-transferrins, catalyzes Cu(+) oxidation and promotes the oxidation of biogenic amines such as norepinephrin and serotonin (By similarity). Provides Cu(2+) ions for the ascorbate-mediated deaminase degradation of the heparan sulfate chains of GPC1 (PubMed : 14707133). Has glutathione peroxidase-like activity, can remove both hydrogen peroxide and lipid hydroperoxide in the presence of thiols. Also shows NO-oxidase and NO2 synthase activities that determine endocrine NO homeostasis (By similarity).
See full target information Ceruloplasmin
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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