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AB155462

Rat CINC-1 ELISA Kit (CXCL1)

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Rat CINC-1 ELISA Kit (CXCL1) is a Sandwich (quantitative) ELISA for the measurement of Rat CINC-1 (CXCL1) in Rat in Biofluids, Cell Culture Media samples.

View Alternative Names

Cinc1, Gro, Scyb1, Cxcl1, Growth-regulated alpha protein, C-X-C motif chemokine 1, Cytokine-induced neutrophil chemoattractant 1, Platelet-derived growth factor-inducible protein KC, CINC-1

2 Images
Sandwich ELISA - Rat CINC-1 ELISA Kit (CXCL1) (AB155462)
  • sELISA

Unknown

Sandwich ELISA - Rat CINC-1 ELISA Kit (CXCL1) (AB155462)

Representative standard curve using ab155462 - Assay Diluent D.

Sandwich ELISA - Rat CINC-1 ELISA Kit (CXCL1) (AB155462)
  • sELISA

Unknown

Sandwich ELISA - Rat CINC-1 ELISA Kit (CXCL1) (AB155462)

Representative standard curve using ab155462 - Assay Diluent B.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 0.05 ng/mL

Range

0.047 - 15 ng/mL

Assay time

5h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's CINC-1 (CXCL-1) Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of rat CINC-1 in serum, plasma and cell culture supernatants. (Collect plasma using EDTA and heparin as an anticoagulant). Citrate is not recommended as anticoagulants for use in this assay. Rat CINC-1 concentration in normal plasma is very low and it may not be detected in this assay.

This assay employs an antibody specific for rat CINC-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and CINC-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat CINC-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CINC-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

We have not been able to detect the endogenous Rat CINC-1 in normal serum with ab155462, only in serum spiked with Rat CINC-1.

Sensitivity of the assay is dependant on the Assay Diluent used. Please refer to Protocol Booklet for more details.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "99 - 122 %", "average": "= 112.5" }, { "sample": "Plasma", "range": "97 - 125 %", "average": "= 107.9" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab155462-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB155462-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB155462-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB155462-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Rat CINC-1 Standard (lyophilized)", "number":"AB155462-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"CINC-1 Microplate (12 x 8 wells)", "number":"AB155462-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB155462-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB155462-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB155462-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-rat CINC-1", "number":"AB155462-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Has chemotactic activity for neutrophils. Contributes to neutrophil activation during inflammation.
See full target information Cxcl1
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websiteProtocolBooklet
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Product promise

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