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AB155463

Rat CINC-3 ELISA Kit (CXCL2)

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(1 Publication)

Rat CINC-3 ELISA Kit (CXCL2) is a Sandwich (quantitative) ELISA for the measurement of Rat CINC-3 (CXCL2) in Rat in Biofluids, Cell Culture Media samples.

View Alternative Names

Cinc3, Mip-2, Mip2, Scyb2, Cxcl2, C-X-C motif chemokine 2, Cytokine-induced neutrophil chemoattractant 3, Macrophage inflammatory protein 2, CINC-3, MIP2

2 Images
Sandwich ELISA - Rat CINC-3 ELISA Kit (CXCL2) (AB155463)
  • sELISA

Unknown

Sandwich ELISA - Rat CINC-3 ELISA Kit (CXCL2) (AB155463)

Representative standard curve using ab155463 - Assay Diluent B.

Sandwich ELISA - Rat CINC-3 ELISA Kit (CXCL2) (AB155463)
  • sELISA

Supplier Data

Sandwich ELISA - Rat CINC-3 ELISA Kit (CXCL2) (AB155463)

Representative standard curve using ab155463 - Assay Diluent C.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

< 2 pg/mL

Range

7.4 - 1800 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's CINC-3 (CXCL2) Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of rat CINC-3 (CXCL2) in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for CINC-3 coated on a 96-well plate. Standards and samples are pipetted into the wells and CINC-3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat CINC-3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CINC-3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Plasma", "range": "72 - 111 %", "average": "= 91.87" }, { "sample": "Cell culture media", "range": "75 - 89 %", "average": "= 83.48" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab155463-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB155463-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB155463-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-rat CINC-3", "number":"AB155463-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB155463-CMP03", "productcode":"" }, { "size":"1 x 200 µL", "name":"600X HRP-Streptavidin Concentrate", "number":"AB155463-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Rat CINC-3 Standard (lyophilized)", "number":"AB155463-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB155463-CMP06", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB155463-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"CINC-3 Microplate (12 x 8 wells)", "number":"AB155463-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Chemotactic for human polymorphonuclear leukocytes but does not induce chemokinesis or an oxidative burst. Contributes to neutrophil activation during inflammation.
See full target information Cxcl2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroimmunology 302:10-19 PubMed27956075

2016

Natural product HTP screening for attenuation of cytokine-induced neutrophil chemo attractants (CINCs) and NO2- in LPS/IFNγ activated glioma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth A Mazzio,David Bauer,Patricia Mendonca,Equar Taka,Karam F A Soliman
View all publications
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websiteProtocolBooklet
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