Rat CINC-3 ELISA Kit (CXCL2) is a Sandwich (quantitative) ELISA kit for the measurement of Rat CINC-3 (CXCL2) in Rat in Plasma, Cell culture supernatant, Serum samples.
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- Journal of neuroimmunology 302:10-192016Natural product HTP screening for attenuation of cytokine-induced neutrophil chemo attractants (CINCs) and NO2- in LPS/IFNγ activated glioma cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElizabeth A Mazzio,David Bauer,Patricia Mendonca,Equar Taka,Karam F A SolimanPubMed 27956075
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 7.4 - 1800 pg/mL
- Sensitivity
- < 2 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Chemotactic for human polymorphonuclear leukocytes but does not induce chemokinesis or an oxidative burst. Contributes to neutrophil activation during inflammation.
Alternative names
Cinc3, Mip-2, Mip2, Scyb2, Cxcl2, C-X-C motif chemokine 2, Cytokine-induced neutrophil chemoattractant 3, Macrophage inflammatory protein 2, CINC-3, MIP2
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Rat CINC-3 ELISA Kit (CXCL2) is a Sandwich (quantitative) ELISA kit for the measurement of Rat CINC-3 (CXCL2) in Rat in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 7.4 - 1800 pg/mL
- Assay Platform
- Microplate
- Sensitivity
- < 2 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 12 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Plasma | Average % = 91.87 | Range 72 - 111 % |
Sample type Cell culture media | Average % = 83.48 | Range 75 - 89 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Abcam's CINC-3 (CXCL2) Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of rat CINC-3 (CXCL2) in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for CINC-3 coated on a 96-well plate. Standards and samples are pipetted into the wells and CINC-3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat CINC-3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CINC-3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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2 product images
Sandwich ELISA - Rat CINC-3 ELISA Kit (CXCL2) (ab155463)
Representative standard curve using ab155463 - Assay Diluent B.
Sandwich ELISA - Rat CINC-3 ELISA Kit (CXCL2) (ab155463)
Representative standard curve using ab155463 - Assay Diluent C.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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