Rat CKM ELISA Kit

Product details

Rat CKM ELISA Kit (ab187396) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CKM protein in serum, tissue extracts, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat CKM with 5.83 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Creatine kinase (CK), also known as creatine phosphokinase (CPK) or phospho-creatine kinase, is an enzyme that catalyzes the reversible transfer of a high-energy phosphate group between phosphocreatine and ATP. There are five known CK isozymes, three located in the cytosol (CKMM, CKMB, CKBB) and two in the mitochondria (Mi _S CK and Mi _u CK). Structurally, cytosolic CK is a 86 kDa dimeric enzyme of two identical or non-identical chains, CKM and/or CKB whereas mitochondrial CK is an octameric enzyme associated with mitochondrial membranes. The compartmentation of CK isoenzymes allows for direct association with ATP-providing or consuming processes both linked via metabolite channeling through the creatine phosphate shuttle. Thus CK isoenzymes allow for the generation of a large pool of phosphocreatine, considered to be a temporal and spatial ATP buffering system in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Expression of cytosolic isoenzyme patterns differ between tissues, with skeletal muscle expressing high levels of CK-MM (98%) and low levels of CK-MB (1%), the myocardium (heart muscle) expressing CK-MM at 70% and CK-MB at 25–30% and the brain and neural tissue expressing mainly CK-BB. CKM is also found in serum from normal animals and levels vary based on muscle mass, age, physical activity and underlying pathologies.

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