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AB100758

Rat CNTF ELISA Kit

3

(1 Review)

|

(1 Publication)

Rat CNTF ELISA Kit is a Sandwich ELISA for the measurement of Rat CNTF in Rat in Biofluids, Cell Culture Media samples.

View Alternative Names

Ciliary neurotrophic factor, CNTF, Cntf

2 Images
Sandwich ELISA - Rat CNTF ELISA Kit (AB100758)
  • sELISA

Unknown

Sandwich ELISA - Rat CNTF ELISA Kit (AB100758)

Representative standard curve using ab100758

Sandwich ELISA - Rat CNTF ELISA Kit (AB100758)
  • sELISA

Unknown

Sandwich ELISA - Rat CNTF ELISA Kit (AB100758)

Representative standard curve using ab100758

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 10 pg/mL

Range

8.23 - 2000 pg/mL

Assay Platform

Microplate

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Proteins & Peptides

AB283456

Recombinant human CNTF protein (Active)

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Functional Studies - Recombinant human CNTF protein (Active) (AB283456)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's CNTF Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat CNTF in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for rat CNTF coated on a 96-well plate. Standards and samples are pipetted into the wells and CNTF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat CNTF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CNTF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "89 - 108 %", "average": "= 97.3" }, { "sample": "Serum", "range": "93 - 112 %", "average": "= 105.6" }, { "sample": "Plasma", "range": "92 - 110 %", "average": "= 104.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100758-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100758-CMP06", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100758-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100758-CMP04", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100758-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-rat CNTF", "number":"AB100758-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"CNTF Microplate (12 strips x 8 wells)", "number":"AB100758-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant rat CNTF Standard (lyophilized)", "number":"AB100758-CMP08", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100758-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB100758-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.
See full target information Cntf
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular physiology 231:1301-12 PubMed26516696

2015

Ex Vivo Assay of Electrical Stimulation to Rat Sciatic Nerves: Cell Behaviors and Growth Factor Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiyong Du,Olexandr Bondarenko,Dingkun Wang,Mahmoud Rouabhia,Ze Zhang
View all publications
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com