JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB285314

Rat Collagen Type I ELISA Kit

Be the first to review this product! Submit a review

|

(2 Publications)

Rat Collagen Type I ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Rat Collagen Type I in Rat in Biofluids, Cell/Tissue Extracts samples.

View Alternative Names

Collagen alpha-1(I) chain, Alpha-1 type I collagen, Col1a1

1 Images
Sandwich ELISA - Rat Collagen Type I ELISA Kit (AB285314)
  • sELISA

Supplier Data

Sandwich ELISA - Rat Collagen Type I ELISA Kit (AB285314)

Rat Collagen Type I ELISA Kit.

Typical Standard Curve : These standard curves are for demonstration only. A standard curve must be run with each assay

Key facts

Detection method

Colorimetric

Sample types

Plasma, Tissue Homogenate, Serum, Other biological fluids

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

< 0.938 ng/mL

Range

1.563 - 100 ng/mL

Assay Platform

Microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Collagen, a major component of the extracellular matrix, is a fibrous protein that provides tensile strength to tissues giving them structural integrity. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function. The extensive family of COL gene products (collagens) is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms.

This COL1 ELISA kit (ab285314, E4619) is a sandwich ELISA assay for the quantitative measurement of COL1 in rat serum, plasma and cell culture supernatants. The density of color is proportional to the amount of COL1 captured from the samples

style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 8" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
style
left-column

What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab285314-96Test", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"25X Wash buffer", "number":"AB285314-CMP12", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Substrate", "number":"AB285314-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Standard", "number":"AB285314-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Micro ELISA Plate", "number":"AB285314-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"Antibody dilution buffer", "number":"AB285314-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"Stop Solution", "number":"AB285314-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample / Standard Dilution Buffer", "number":"AB285314-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"SABC dilution buffer", "number":"AB285314-CMP06", "productcode":"" }, { "size":"1 x 120 µL", "name":"HRP-Streptavidin Conjugate (SABC)", "number":"AB285314-CMP10", "productcode":"" }, { "size":"1 x 120 µL", "name":"Biotin/Detection antibody (Concentrated)", "number":"AB285314-CMP02", "productcode":"" }, { "size":"5 x 1 Unit", "name":"Plate Sealer", "number":"AB285314-CMP04", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Collagen type I also called collagen I is a structural protein expressed mainly in connective tissues such as skin tendon bone and ligaments. It serves as an important component in providing mechanical strength and integrity to these tissues. Collagen I is a fibrillar collagen known for its triple-helix structure composed of two alpha-1 chains and one alpha-2 chain and has a molecular mass of approximately 300 kDa. Researchers often employ collagen western blot and collagen ELISA techniques for its detection. Collagen suppliers offer various collagen antibodies used in these assays to study its distribution and function.
Biological function summary

Collagen type I plays a central role in maintaining the extracellular matrix and supporting cellular environments. It interacts with other matrix proteins and cells forming complexes that help in tissue development and repair. Type I collagen is especially important in bone matrix working alongside minerals like hydroxyapatite to provide rigidity and support. Anti-collagen antibodies aid in studying its biological functions and interactions which are critical to understanding tissue dynamics.

Pathways

Collagen type I interacts with multiple signaling cascades involved in tissue remodeling and repair. It is a significant player in the TGF-β pathway which regulates fibrosis and wound healing processes. In these pathways proteins such as fibronectin and integrins work in concert with collagen type I to orchestrate cellular responses to damage. Researchers often examine its role in these pathways to uncover therapeutic possibilities for disease interventions.

Collagen type I has strong connections to conditions like osteogenesis imperfecta and fibrosis. Mutations or irregularities in collagen I production can lead to osteogenesis imperfecta a genetic disorder characterized by brittle bones. In fibrosis excessive collagen deposition disrupts normal tissue architecture contributing to organ dysfunction. In both conditions type I collagen interacts with other proteins like matrix metalloproteinases which modulate its breakdown and remodeling highlighting its importance in disease pathology.
style
left-column

Product protocols

style
left-column

Target data

Type I collagen is a member of group I collagen (fibrillar forming collagen).
See full target information Col1a1
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 12:1455258 PubMed39839674

2025

Relevance of the GH-VEGFB/VEGFA axis in liver grafts from brain-dead donors with alcohol-associated liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Marc Micó-Carnero,Carlos Rojano-Alfonso,Cristina Maroto-Serrat,Juan Carlos Cutrin,Araní Casillas-Ramírez,Carmen Peralta

Heliyon 8:e10480 PubMed36105473

2022

Experimental study on the effects of simvastatin in reversing the femoral metaphyseal defects induced by sodium valproate in normal and ovariectomized rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Li,Ruotian Zhang,Maoxian Ren,Hedong Liu,Min Yang
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB108850

Rat Fibronectin ELISA Kit

Sandwich ELISA - Rat Fibronectin ELISA Kit (AB108850)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Proteins & Peptides

AB50036

Recombinant human TGF beta 1 protein (Active)

Bioactive

  • 0
  • 0
Assay Kits

AB275897

Rat CTGF ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Rat CTGF ELISA Kit (AB275897)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com