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Rat Creatine Kinase MB ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Rat Creatine Kinase MB in Plasma, Tissue Homogenate, Serum samples.

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Sandwich ELISA - Rat Creatine Kinase MB ELISA Kit (AB285275), expandable thumbnail

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Key facts

Detection method
Colorimetric
Sample types
Plasma, Tissue Homogenate, Serum
Assay type
Sandwich (quantitative)
Range
31.25 - 2000 pg/mL
Sensitivity
< 18.75 pg/mL

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Function

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Alternative names

Ckmm, Ckm, Creatine kinase M-type, Creatine kinase M chain, Creatine phosphokinase M-type, M-CK, CPK-M

What's included?

96 Test
Components
25X Wash buffer
1 x 30 mL
Antibody dilution buffer
1 x 10 mL
Biotin/Detection antibody (Concentrated)
1 x 120 µL
HRP-Streptavidin Conjugate (SABC)
1 x 120 µL
Lyophilized Standard
2 x 1 Vial
Micro ELISA Plate
1 x 1 Unit
Plate Sealer
5 x 1 Unit
SABC dilution buffer
1 x 10 mL
Sample / Standard Dilution Buffer
1 x 20 mL
Stop Solution
1 x 10 mL
TMB Substrate
1 x 10 mL

Recommended products

Rat Creatine Kinase MB ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Rat Creatine Kinase MB in Plasma, Tissue Homogenate, Serum samples.

Key facts

Detection method
Colorimetric
Sample types
Plasma, Tissue Homogenate, Serum
Assay type
Sandwich (quantitative)
Range
31.25 - 2000 pg/mL
Assay Platform
Pre-coated microplate (12 x 8 well strips)
Sensitivity
< 18.75 pg/mL

Precision

Intra assay

Sample
General
n
0
mean
-
SD
-
C.V.
< 8

Inter assay

Sample
General
n
0
mean
-
SD
-
C.V.
< 10

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Notes

Creatine Kinase (CK) also known as creatine phosphokinase (CPK) and ATP: creatine N-phosphotransferase is a common cellular enzyme. It catalyzes the reversible conversion of creatine and ATP into ADP and phosphocreatine. CK is widely expressed in various tissues and cell types, with highest activity in striated muscles, heart tissue and brain. CK consists of two subunits: M (muscle) and B (brain), and has three isoenzymes: CK-MM (skeleton muscle), CK-MB (cardiac muscle), and CK-BB (brain). Increased CK level is associated with many diseases such as myocardial infarction, muscular dystrophy, pulmonary infarction and brain tumors. Accurate measurement of CK is crucial for early diagnosis, prediction and therapeutic strategy. This CK-MB ELISA kit (ab285275, E4608) is a sandwich ELISA assay for the quantitative measurement of CK-MB in rat serum, plasma and cell culture supernatants. The density of color is proportional to the amount of CK-MB captured from the samples.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Rat Creatine Kinase MB ELISA Kit (ab285275), expandable thumbnail

    Sandwich ELISA - Rat Creatine Kinase MB ELISA Kit (ab285275)

    Standard Rat CK-MB Curve.

    Typical Standard Curve: These standard curves are for demonstration only. A standard curve must be run with each assay.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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