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AB285275

Rat Creatine Kinase MB ELISA Kit

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(4 Publications)

Rat Creatine Kinase MB ELISA Kit is a Sandwich ELISA for the measurement of Rat Creatine Kinase MB in in Biofluids, Cell/Tissue Extracts samples.

View Alternative Names

Ckmm, Ckm, Creatine kinase M-type, Creatine kinase M chain, Creatine phosphokinase M-type, M-CK, CPK-M

1 Images
Sandwich ELISA - Rat Creatine Kinase MB ELISA Kit (AB285275)
  • sELISA

Supplier Data

Sandwich ELISA - Rat Creatine Kinase MB ELISA Kit (AB285275)

Standard Rat CK-MB Curve.

Typical Standard Curve : These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Tissue Homogenate, Serum

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 18.75 pg/mL

Range

31.25 - 2000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Creatine Kinase (CK) also known as creatine phosphokinase (CPK) and ATP: creatine N-phosphotransferase is a common cellular enzyme. It catalyzes the reversible conversion of creatine and ATP into ADP and phosphocreatine. CK is widely expressed in various tissues and cell types, with highest activity in striated muscles, heart tissue and brain. CK consists of two subunits: M (muscle) and B (brain), and has three isoenzymes: CK-MM (skeleton muscle), CK-MB (cardiac muscle), and CK-BB (brain). Increased CK level is associated with many diseases such as myocardial infarction, muscular dystrophy, pulmonary infarction and brain tumors. Accurate measurement of CK is crucial for early diagnosis, prediction and therapeutic strategy. This CK-MB ELISA kit (ab285275, E4608) is a sandwich ELISA assay for the quantitative measurement of CK-MB in rat serum, plasma and cell culture supernatants. The density of color is proportional to the amount of CK-MB captured from the samples.
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Precision

[ { "reproducibilityType": "Inter", "sample": "General", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Intra", "sample": "General", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 8" } ]
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What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab285275-96Test", "assetComponentDetails": [ { "size":"1 x 10 mL", "name":"Antibody dilution buffer", "number":"AB285275-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Substrate", "number":"AB285275-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"Stop Solution", "number":"AB285275-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample / Standard Dilution Buffer", "number":"AB285275-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"SABC dilution buffer", "number":"AB285275-CMP06", "productcode":"" }, { "size":"1 x 120 µL", "name":"HRP-Streptavidin Conjugate (SABC)", "number":"AB285275-CMP09", "productcode":"" }, { "size":"1 x 120 µL", "name":"Biotin/Detection antibody (Concentrated)", "number":"AB285275-CMP02", "productcode":"" }, { "size":"5 x 1 Unit", "name":"Plate Sealer", "number":"AB285275-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Micro ELISA Plate", "number":"AB285275-CMP03", "productcode":"" }, { "size":"1 x 30 mL", "name":"25X Wash buffer", "number":"AB285275-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Standard", "number":"AB285275-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
See full target information Ckm
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 23:439 PubMed40514650

2025

Nanoscale engineered exosomes for dual delivery of Sirtuin3 and insulin to ignite mitochondrial recovery in myocardial ischemia-reperfusion.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaxin Yang,Xinyi Yun,Weihan Zheng,Huihui Zhang,Zi Yan,Youyu Chen,Wanting Xue,Siqi Mi,Ziyue Li,Hanxiao Sun,Guozhi Xiao,Zhenning Dai,Shiyu Li,Wenhua Huang

Folia morphologica : PubMed40145712

2025

Cardioprotective effect of phytosterol stigmasterol supplementation against doxorubicin-induced cardiotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Wang

Open veterinary journal 13:1718-1728 PubMed38292716

2024

The role of hesperidin as a cardioprotective strategy against doxorubicin-induced cardiotoxicity: The antioxidant, anti-inflammatory, antiapoptotic, and cytoprotective potentials.

Applications

Unspecified application

Species

Unspecified reactive species

Fawiziah Khalaf Alharbi,Zafer S Alshehri,Faez F Alshehri,Sharif Alhajlah,Hesham A Khalifa,Naief Dahran,Wael A M Ghonimi

International heart journal 64:935-944 PubMed37778997

2023

Exosomes from Adipose-Derived Mesenchymal Stem Cells Protect Against Cyclophosphamide-Induced Cardiotoxicity in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Jianhua Xiong,Binjun Ding,Wei Zhu,Lanlan Xu,Songping Yu
View all publications
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Product promise

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