Rat CRP ELISA Kit (C-Reactive Protein), 90-min ELISA (ab256398)

Rat CRP ELISA Kit (C-Reactive Protein) is a single-wash 90-min Simplestep used to quantify Rat CRP (C-Reactive Protein) with a sensitivity of 3.9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

Be the first to review this product! Submit a review

Images

Sandwich ELISA - Rat CRP ELISA Kit (C-Reactive Protein) (AB256398), expandable thumbnail

Publications

Key facts

Detection method: Colorimetric
Sample types: Urine, Tissue Extracts, Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
Assay type: Sandwich (quantitative)
Reactive species: Rat
Range: 19.5 - 1250 pg/mL
Assay time: 1h 30m
Sensitivity: = 3.9 pg/mL

Reactivity data

Application	Reactivity	Dilution info	Notes
Application sELISA	Reactivity Reacts	Dilution info -	Notes -

Target data

See full target information Crp

Function

Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells (By similarity).

Alternative names

Ptx1, Crp, C-reactive protein

What's included?

1 x 384 Tests

Components

10X Rat CRP (C-Reactive protein) Capture Antibody

1 x 600 µL

10X Rat CRP (C-Reactive protein) Detector Antibody

1 x 600 µL

10X Wash Buffer PT (ab206977)

2 x 20 mL

384 well CaptSure™ microplates

1 x 1 Unit

50X Cell Extraction Enhancer Solution (ab193971)

1 x 5 mL

5X Cell Extraction Buffer PTR (ab193970)

1 x 50 mL

Antibody Diluent 4BR

1 x 6 mL

Plate Seals

1 x 1 Unit

Rat CRP (C-Reactive protein) Lyophilized Recombinant Protein

2 x 1 Vial

Sample Diluent NS (ab193972)

1 x 250 mL

Stop Solution

2 x 12 mL

TMB Development Solution

2 x 12 mL

Key facts

Detection method: Colorimetric
Sample types: Urine, Tissue Extracts, Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
Assay type: Sandwich (quantitative)
Reactive species: Rat
Range: 19.5 - 1250 pg/mL
Assay time: 1h 30m
Assay Platform: Pre-coated microplate (12 x 8 well strips)
Sensitivity: = 3.9 pg/mL

Precision

Intra assay

Sample	n	C.V.
Sample Extract	n 8	C.V. 4.2

Inter assay

Sample	n	C.V.
Sample Extract	n 3	C.V. 4.4

Recovery

Sample specific recovery

Sample type	Average %	Range
Sample type Cell culture supernatant	Average % = 99	Range 97 - 101 %
Sample type Serum	Average % = 103	Range 103 - 104 %
Sample type EDTA Plasma	Average % = 111	Range 103 - 118 %
Sample type Urine	Average % = 94	Range 90 - 98 %
Sample type Cell culture media	Average % = 113	Range 112 - 115 %
Sample type Tissue Extracts	Average % = 105	Range 101 - 109 %
Sample type Heparin Plasma	Average % = 117	Range 116 - 119 %
Sample type Citrate plasma	Average % = 114	Range 109 - 124 %

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: +4°C

Notes

Rat CRP ELISA Kit (C-Reactive Protein) (ab256398) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CRP (C-Reactive Protein) protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, urine, tissue extracts, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat CRP (C-Reactive Protein) with 3.9 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

CRP (C-Reactive Protein) displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. CRP can interact with DNA and histones and it may scavenge nuclear material released from damaged circulations cells. CRP is secreted; it forms a homopentamer pentaxin (or pentraxin) which have a discoid arrangement of 5 non-covalently bound subunits. CRP binds 2 calcium ions per subunit. The concentration of CRP in plasma increases greatly during acute phase response to tissue injury, infection or other inflammatory stimuli. It is induced by IL1/interleukin-1 and IL6/interleukin-6.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

C-reactive protein (CRP) also known as CRP protein is an acute-phase protein with a molecular mass of approximately 25 kDa. It is primarily synthesized in the liver in response to cytokines. This protein appears in the blood where it binds to phosphocholine expressed on the surface of dead or dying cells. CRP is often used as a biomarker for inflammation in the body and its levels in human and canine (anti CRP used) blood samples are measured using assays like the CRP ELISA test or CRP ELISA kit.

Biological function summary

CRP plays a significant role in the innate immune system by recognizing foreign pathogens and damaged cells. It does not form part of a larger complex but itself can bind to specific ligands initiating the complement cascade. This activation enhances phagocytosis by macrophages contributing to the clearance of necrotic and apoptotic cells. The CRP protein therefore acts as a fast responder in the presence of inflammation making it a useful indicator for monitoring inflammatory diseases.

Pathways

CRP operates predominantly within the acute-phase response pathway. Its production is upregulated by cytokines such as IL-6 which connects it to inflammation-signaling pathways. CRP also interacts with the complement system bridging innate and adaptive immunity. Besides CRP could indirectly influence pathways involving other inflammatory proteins like serum amyloid A and haptoglobin creating a network of responses important for managing inflammation.

Associated diseases and disorders

Elevated CRP levels are commonly associated with cardiovascular diseases and rheumatoid arthritis. In cardiovascular diseases CRP acts as a marker for atherosclerosis where the protein levels correlate with disease severity. It connects with other proteins involved in inflammatory processes such as fibrinogen to contribute to disease pathology. In rheumatoid arthritis CRP provides insights into disease activity helping in the assessment of treatment responses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

Sandwich ELISA - Rat CRP ELISA Kit (C-Reactive Protein) (ab256398)
Example of rat CRP standard curve in Sample Diluent NS.
The CRP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Rat CRP ELISA Kit (C-Reactive Protein) (ab256398)
Interpolated concentrations of native CRP in rat serum and plasma samples.
The concentrations of CRP were measured in duplicates, interpolated from the CRP standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:400,000, plasma (citrate) 1:200,000, plasma (heparin) 1:400,000, and plasma (EDTA) 1:200,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CRP concentration was determined to be 326.3 μg/mL in neat serum, 170.1 μg/mL in neat plasma (citrate), 307.1 μg/mL in neat plasma (heparin), and 231.1 μg/mL in neat plasma (EDTA).
Sandwich ELISA - Rat CRP ELISA Kit (C-Reactive Protein) (ab256398)
Interpolated concentrations of native CRP in rat urine and tissue culture supernatant samples.
The concentrations of CRP were measured in duplicates, interpolated from the CRP standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 1:50, rat heart tissue supernatant 1:200, and rat spleen tissue supernatant 1:200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CRP concentration was determined to be 36.29 ng/mL in neat urine, 105.0 ng/mL in neat rat heart tissue supernatant, and 87.24 ng/mL in neat rat spleen tissue supernatant.
Sandwich ELISA - Rat CRP ELISA Kit (C-Reactive Protein) (ab256398)
Interpolated concentration of native CRP was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1:400,000 rat serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Rat CRP ELISA Kit (C-Reactive Protein) (ab256398)
Example of rat CRP standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Rat CRP ELISA Kit (C-Reactive Protein) (ab256398)
Interpolated concentrations of native CRP in rat heart tissue extract based on a 1 μg/mL extract load.
The concentrations of CRP were measured in duplicate and interpolated from the CRP standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CRP concentration was determined to be 359.0 pg/mL in rat heart tissue extract.
Sandwich ELISA - Rat CRP ELISA Kit (C-Reactive Protein) (ab256398)
Interpolated concentration of native CRP was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 µg/mL rat heart tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
Sandwich ELISA - Rat CRP ELISA Kit (C-Reactive Protein) (ab256398)
Example of rat CRP standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.