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AB256398

Rat CRP ELISA Kit (C-Reactive Protein)

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(7 Publications)

Rat CRP ELISA Kit (C-Reactive Protein) is a single-wash 90-min Simplestep used to quantify Rat CRP (C-Reactive Protein) with a sensitivity of 3.9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

View Alternative Names

Ptx1, Crp, C-reactive protein

9 Images
ELISA - Rat CRP ELISA Kit (C-Reactive Protein) (AB256398)
  • ELISA

Supplier Data

ELISA - Rat CRP ELISA Kit (C-Reactive Protein) (AB256398)
Sandwich ELISA - Rat CRP ELISA Kit (C-Reactive Protein) (AB256398)
  • sELISA

Supplier Data

Sandwich ELISA - Rat CRP ELISA Kit (C-Reactive Protein) (AB256398)

Interpolated concentrations of native CRP in rat urine and tissue culture supernatant samples.

The concentrations of CRP were measured in duplicates, interpolated from the CRP standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 1 : 50, rat heart tissue supernatant 1 : 200, and rat spleen tissue supernatant 1 : 200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CRP concentration was determined to be 36.29 ng/mL in neat urine, 105.0 ng/mL in neat rat heart tissue supernatant, and 87.24 ng/mL in neat rat spleen tissue supernatant.

Sandwich ELISA - Rat CRP ELISA Kit (C-Reactive Protein) (AB256398)
  • sELISA

Supplier Data

Sandwich ELISA - Rat CRP ELISA Kit (C-Reactive Protein) (AB256398)

Interpolated concentrations of native CRP in rat serum and plasma samples.

The concentrations of CRP were measured in duplicates, interpolated from the CRP standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 400,000, plasma (citrate) 1 : 200,000, plasma (heparin) 1 : 400,000, and plasma (EDTA) 1 : 200,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CRP concentration was determined to be 326.3 μg/mL in neat serum, 170.1 μg/mL in neat plasma (citrate), 307.1 μg/mL in neat plasma (heparin), and 231.1 μg/mL in neat plasma (EDTA).

Sandwich ELISA - Rat CRP ELISA Kit (C-Reactive Protein) (AB256398)
  • sELISA

Supplier Data

Sandwich ELISA - Rat CRP ELISA Kit (C-Reactive Protein) (AB256398)

Interpolated concentrations of native CRP in rat heart tissue extract based on a 1 μg/mL extract load.

The concentrations of CRP were measured in duplicate and interpolated from the CRP standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CRP concentration was determined to be 359.0 pg/mL in rat heart tissue extract.

Sandwich ELISA - Rat CRP ELISA Kit (C-Reactive Protein) (AB256398)
  • sELISA

Supplier Data

Sandwich ELISA - Rat CRP ELISA Kit (C-Reactive Protein) (AB256398)

Example of rat CRP standard curve in Sample Diluent NS.

The CRP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Rat CRP ELISA Kit (C-Reactive Protein) (AB256398)
  • sELISA

Lab

Sandwich ELISA - Rat CRP ELISA Kit (C-Reactive Protein) (AB256398)

Interpolated concentration of native CRP was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 µg/mL rat heart tissue extract.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Rat CRP ELISA Kit (C-Reactive Protein) (AB256398)
  • sELISA

Lab

Sandwich ELISA - Rat CRP ELISA Kit (C-Reactive Protein) (AB256398)

Example of rat CRP standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Rat CRP ELISA Kit (C-Reactive Protein) (AB256398)
  • sELISA

Lab

Sandwich ELISA - Rat CRP ELISA Kit (C-Reactive Protein) (AB256398)

Example of rat CRP standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Rat CRP ELISA Kit (C-Reactive Protein) (AB256398)
  • sELISA

Lab

Sandwich ELISA - Rat CRP ELISA Kit (C-Reactive Protein) (AB256398)

Interpolated concentration of native CRP was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 400,000 rat serum.  The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Colorimetric

Sample types

Urine, Tissue Extracts, Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 3.9 pg/mL

Range

19.5 - 1250 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Rat CRP ELISA Kit (C-Reactive Protein) ab256398 is a rapid single-wash 90-min Sandwich ELISA to measure Rat CRP (C-Reactive Protein)in cell culture media, cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum, tissue extracts, urine. This SimpleStep sensitivity is 3.9 pg/mL.

How the assay works

Rat CRP (C-Reactive Protein)SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Rat CRP ELISA Kit (C-Reactive Protein) ab256398 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-C Reactive Protein antibody [EPR22096-81] - BSA and Azide free (Capture) ab259718
- Anti-C Reactive Protein antibody [EPR22096-258] - BSA and Azide free (Detector) ab259718

CRP (C-Reactive Protein) displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. CRP can interact with DNA and histones and it may scavenge nuclear material released from damaged circulations cells. CRP is secreted; it forms a homopentamer pentaxin (or pentraxin) which have a discoid arrangement of 5 non-covalently bound subunits. CRP binds 2 calcium ions per subunit. The concentration of CRP in plasma increases greatly during acute phase response to tissue injury, infection or other inflammatory stimuli. It is induced by IL1/interleukin-1 and IL6/interleukin-6.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.4" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.2" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "97 - 101 %", "average": "= 99" }, { "sample": "Serum", "range": "103 - 104 %", "average": "= 103" }, { "sample": "EDTA Plasma", "range": "103 - 118 %", "average": "= 111" }, { "sample": "Urine", "range": "90 - 98 %", "average": "= 94" }, { "sample": "Cell culture media", "range": "112 - 115 %", "average": "= 113" }, { "sample": "Tissue Extracts", "range": "101 - 109 %", "average": "= 105" }, { "sample": "Heparin Plasma", "range": "116 - 119 %", "average": "= 117" }, { "sample": "Citrate plasma", "range": "109 - 124 %", "average": "= 114" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C-reactive protein (CRP) also known as CRP protein is an acute-phase protein with a molecular mass of approximately 25 kDa. It is primarily synthesized in the liver in response to cytokines. This protein appears in the blood where it binds to phosphocholine expressed on the surface of dead or dying cells. CRP is often used as a biomarker for inflammation in the body and its levels in human and canine (anti CRP used) blood samples are measured using assays like the CRP ELISA test or CRP ELISA kit.
Biological function summary

CRP plays a significant role in the innate immune system by recognizing foreign pathogens and damaged cells. It does not form part of a larger complex but itself can bind to specific ligands initiating the complement cascade. This activation enhances phagocytosis by macrophages contributing to the clearance of necrotic and apoptotic cells. The CRP protein therefore acts as a fast responder in the presence of inflammation making it a useful indicator for monitoring inflammatory diseases.

Pathways

CRP operates predominantly within the acute-phase response pathway. Its production is upregulated by cytokines such as IL-6 which connects it to inflammation-signaling pathways. CRP also interacts with the complement system bridging innate and adaptive immunity. Besides CRP could indirectly influence pathways involving other inflammatory proteins like serum amyloid A and haptoglobin creating a network of responses important for managing inflammation.

Elevated CRP levels are commonly associated with cardiovascular diseases and rheumatoid arthritis. In cardiovascular diseases CRP acts as a marker for atherosclerosis where the protein levels correlate with disease severity. It connects with other proteins involved in inflammatory processes such as fibrinogen to contribute to disease pathology. In rheumatoid arthritis CRP provides insights into disease activity helping in the assessment of treatment responses.
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Product protocols

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Target data

Displays several functions associated with host defense : it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells (By similarity).
See full target information Crp
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cell journal 26:473-486 PubMed39380478

2024

Therapeutic Effects of and Metformin on Estradiol-Induced Polycystic Ovary Syndrome in Rats: Insights into The PI3K/AKT Pathway, Inflammation, and Oxidative Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Jiefan Gao,Yue Huang,Yangyang Song,Xiao Huang,Dan Zhang,Zhimin Hou

Brain sciences 13: PubMed37508953

2023

Standardizing an Experimental Murine Model of Extraparenchymal Neurocysticercosis That Immunologically Resembles Human Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Alejandro Espinosa-Cerón,Alejandro Méndez,Juan Hernández-Aceves,Juan C Juárez-González,Nelly Villalobos,Marisela Hernández,Georgina Díaz,Paola Soto,Luis Concha,Iván N Pérez-Osorio,Juan J Ortiz-Retana,Raúl J Bobes,Robert M Parkhouse,P T Hamamoto Filho,Gladis Fragoso,Edda Sciutto

Nutrients 15: PubMed37447254

2023

Bean Leaves Ameliorate Lipotoxicity in Fatty Liver Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Adriana Araceli Becerril-Campos,Minerva Ramos-Gómez,Ericka Alejandra De Los Ríos-Arellano,Perla Viridiana Ocampo-Anguiano,Adriana González-Gallardo,Yazmín Macotela,Teresa García-Gasca,Santiaga Marisela Ahumada-Solórzano

Nutrients 14: PubMed35565759

2022

Anti-Hyperlipidemia, Hypoglycemic, and Hepatoprotective Impacts of Pearl Millet ( L.) Grains and Their Ethanol Extract on Rats Fed a High-Fat Diet.

Applications

Unspecified application

Species

Unspecified reactive species

Nadiah S Alzahrani,Ghedeir M Alshammari,Afaf El-Ansary,Abu ElGasim A Yagoub,Musarat Amina,Ali Saleh,Mohammed Abdo Yahya

International journal of molecular sciences 22: PubMed34638520

2021

Detection and Evaluation of Serological Biomarkers to Predict Osteoarthritis in Anterior Cruciate Ligament Transection Combined Medial Meniscectomy Rat Model.

Applications

Unspecified application

Species

Unspecified reactive species

Nian-Cih Huang,Tsorng-Shyang Yang,Prabhakar Busa,Ching-Ling Lin,Ya-Chieh Fang,Ing-Jung Chen,Chih-Shung Wong

Cytotechnology 73:727-743 PubMed34629748

2021

miR-200b-3p alleviates TNF-α-induced apoptosis and inflammation of intestinal epithelial cells and ulcerative colitis progression in rats via negatively regulating KHDRBS1.

Applications

Unspecified application

Species

Unspecified reactive species

Chunju Zheng,Ting Lu,Zhimin Fan

International journal of molecular sciences 20: PubMed31731607

2019

Calcium Phosphate Bions Cause Intimal Hyperplasia in Intact Aortas of Normolipidemic Rats through Endothelial Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Daria Shishkova,Elena Velikanova,Maxim Sinitsky,Anna Tsepokina,Olga Gruzdeva,Leo Bogdanov,Anton Kutikhin
View all publications
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