AB219044

Rat CXCL1 ELISA Kit (GRO alpha)

Recombinant
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(2 Publications)

Rat CXCL1 ELISA Kit (GRO alpha) is a Sandwich (quantitative) ELISA for the measurement of Rat CXCL1 (GRO alpha) in Rat in Cell/Tissue Extracts, Biofluids, Cell Culture Media samples.

View Alternative Names

Cinc1, Gro, Scyb1, Cxcl1, Growth-regulated alpha protein, C-X-C motif chemokine 1, Cytokine-induced neutrophil chemoattractant 1, Platelet-derived growth factor-inducible protein KC, CINC-1

8 Images

Sandwich ELISA - Rat CXCL1 ELISA Kit (GRO alpha) (AB219044)

sELISA

Supplier Data

Sandwich ELISA - Rat CXCL1 ELISA Kit (GRO alpha) (AB219044)

Example of rat CXCL1 (GRO alpha) standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Rat CXCL1 ELISA Kit (GRO alpha) (AB219044)

Interpolated concentrations of native CXCL1 (GRO alpha) in rat serum and plasma samples.

The concentrations of CXCL1 (GRO alpha) were measured in duplicates, interpolated from the CXCL1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL1 concentration was determined to be 245.16 pg/mL in neat serum, 75.79 pg/mL in neat plasma (citrate), 24.53 pg/mL in neat plasma (EDTA), and 137.19 pg/mL in neat plasma (heparin).

sELISA

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Sandwich ELISA - Rat CXCL1 ELISA Kit (GRO alpha) (AB219044)

Interpolated concentrations of spiked CXCL1 (GRO alpha) in rat serum and plasma samples.

The concentrations of CXCL1 (GRO alpha) were measured in duplicates, interpolated from the CXCL1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL1 concentration was determined to be 930.66 pg/mL in neat serum, 756.22 pg/mL in neat plasma (citrate), 674.04 pg/mL in neat plasma (EDTA), and 891.86 pg/mL in neat plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Rat CXCL1 ELISA Kit (GRO alpha) (AB219044)

Interpolated concentrations of native CXCL1 (GRO alpha) in rat C6 cell extract based on a 500 μg/mL extract load.

The concentrations of CXCL1 (GRO alpha) were measured in duplicate and interpolated from the CXCL1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL1 concentration was determined to be 532.46 pg/mL in C6 cell extract.

sELISA

Supplier Data

Sandwich ELISA - Rat CXCL1 ELISA Kit (GRO alpha) (AB219044)

Interpolated concentrations of native CXCL1 (GRO alpha) in rat tissue culture supernatant samples. Interpolated concentrations of native CXCL1 in rat tissue culture supernatant samples. The concentrations of CXCL1 were measured in duplicates, interpolated from the CXCL1 standard curves and corrected for sample dilution. Undiluted samples are as follows : rat lung supernatant 1% and rat spleen supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL1 concentration was determined to be 57,710 pg/mL in neat rat lung supernatant and 838 pg/mL in neat rat spleen supernatant. The rat lung supernatant was cultured for six days in RPMI base media with 10% Fetal Bovine Serum (FBS) plus 5 µg/mL of LPS. The rat spleen supernatant was cultured for three days in RPMI base media with 10% FBS.

sELISA

Supplier Data

Sandwich ELISA - Rat CXCL1 ELISA Kit (GRO alpha) (AB219044)

Rat CXCL1 (GRO alpha) standard curve comparison data. Standard curve comparison between rat CXCL1 SimpleStep ELISA® kit (GRO alpha) and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Rat CXCL1 ELISA Kit (GRO alpha) (AB219044)

Example of rat CXCL1 (GRO alpha) standard curve. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Rat CXCL1 ELISA Kit (GRO alpha) (AB219044)

Example of rat CXCL1 (GRO alpha) standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 4.39 pg/mL

Range

18.75 - 1200 pg/mL

Assay time

1h 45m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Rat CXCL1 ELISA Kit (GRO alpha) (ab219044) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CXCL1 (GRO alpha) protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat CXCL1 with 1.61 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

CXCL1 (previously called GRO1 oncogene) is a member of the CXC family of chemokines. CXCL1 expression is reported in lymphocytes such as macrophages and neutrophils as well as epithelial cells. It plays a role in neutrophil chemotaxis. CXCL1 is involved in spinal cord development via inhibition of oligodendrocyte precursor migration as well angiogenesis, arteriogenesis, inflammation, wound healing and tumorigenesis.

Rat CXCL2 and CXCL3 share 65% and 64% sequence homology with rat CXCL1, respectively. Additionally, mouse CXCL1 is 89% homologous with rat CXCL1.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Rat Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10" }, { "reproducibilityType": "Intra", "sample": "Rat Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.1" } ]

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Recovery

[ { "sample": "Serum", "range": "88 - 109 %", "average": "= 99" }, { "sample": "EDTA Plasma", "range": "103 - 108 %", "average": "= 106" }, { "sample": "Cell culture extracts", "range": "100 - 104 %", "average": "= 103" }, { "sample": "Heparin Plasma", "range": "110 - 118 %", "average": "= 114" }, { "sample": "Citrate plasma", "range": "88 - 112 %", "average": "= 97" }, { "sample": "Cell culture media", "range": "90 - 102 %", "average": "= 95" }, { "sample": "Tissue Culture Media", "range": "85 - 116 %", "average": "= 96" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab219044-1x96Tests", "assetComponentDetails": [ { "size":"2 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB219044-CMP13", "productcode":"" }, { "size":"2 x 20 mL", "name":"10X Wash Buffer PT", "number":"AB219044-CMP12", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat CXCL1 Capture Antibody", "number":"AB219044-CMP09", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB219044-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rat CXCL1 Lyophilized Recombinant Protein", "number":"AB219044-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB219044-CMP08", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB219044-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat CXCL1 Detector Antibody", "number":"AB219044-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB219044-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB219044-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB219044-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB219044-CMP06", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CXCL1 also known as GRO alpha or KC in certain species is a chemokine with a molecular mass of around 7.8 kDa. This protein is primarily secreted by macrophages neutrophils and endothelial cells. It plays a critical role in the recruitment of neutrophils during inflammation and acts by binding to the CXCR2 receptor. CXCL1 is expressed in many tissues including lungs liver and skin where it regulates the immune response and facilitates tissue repair.

Biological function summary

CXCL1 functions as a significant mediator in inflammatory processes. It does not form a complex but acts independently to exert its effects. By attracting neutrophils to sites of injury or infection CXCL1 supports the body's defense mechanisms. This chemokine also influences angiogenesis contributing to the formation of new blood vessels which is essential in wound healing and tissue regeneration.

Pathways

CXCL1 plays a role in both the NF-kB signaling and MAPK signaling pathways. These pathways are pivotal for the regulation of immune and inflammatory responses. CXCL1 acts alongside proteins such as IL-8 another chemokine that also binds to the CXCR2 receptor. Through these pathways CXCL1 influences the activation and migration of immune cells facilitating a rapid response to inflammatory stimuli.

CXCL1 has a significant association with conditions such as chronic obstructive pulmonary disease (COPD) and rheumatoid arthritis. In COPD elevated levels of CXCL1 contribute to persistent inflammation and neutrophil infiltration. In rheumatoid arthritis CXCL1 participates in joint inflammation and damage interacting with proteins like TNF-alpha which amplifies inflammatory responses. Understanding the role of CXCL1 in these conditions could help guide the development of targeted therapies to mitigate inflammation and tissue damage.

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Product protocols

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Target data

Has chemotactic activity for neutrophils. Contributes to neutrophil activation during inflammation.

See full target information Cxcl1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 23:226 PubMed35222703

2022

Inhibition of thioredoxin-interacting protein may enhance the therapeutic effect of dehydrocostus lactone in cardiomyocytes under doxorubicin stimulation via the inhibition of the inflammatory response.

Applications

Unspecified application

Species

Unspecified reactive species

Xuezhi Zhang,Cuiyu Chu,Yuankun Huang

Experimental and therapeutic medicine 17:4687-4692 PubMed31086602

2019

Protective effects of Asiatic acid against pelvic inflammatory disease in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Dejia Kong,Ping Fu,Qin Zhang,Xian Ma,Ping Jiang

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rat CXCL1 ELISA Kit (GRO alpha)

Sandwich ELISA - Rat CXCL1 ELISA Kit (GRO alpha) (AB219044)

Sandwich ELISA - Rat CXCL1 ELISA Kit (GRO alpha) (AB219044)

Sandwich ELISA - Rat CXCL1 ELISA Kit (GRO alpha) (AB219044)

Sandwich ELISA - Rat CXCL1 ELISA Kit (GRO alpha) (AB219044)

Sandwich ELISA - Rat CXCL1 ELISA Kit (GRO alpha) (AB219044)

Sandwich ELISA - Rat CXCL1 ELISA Kit (GRO alpha) (AB219044)

Sandwich ELISA - Rat CXCL1 ELISA Kit (GRO alpha) (AB219044)

Sandwich ELISA - Rat CXCL1 ELISA Kit (GRO alpha) (AB219044)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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