Rat CXCL5 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Rat CXCL5 in Rat in Cell/Tissue Extracts samples.
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AMCF II, C-X-C motif chemokine 5, ENA 78, Epithelial-derived neutrophil-activating protein 78, LIX, Neutrophil-activating peptide ENA-78, SCYB5, SCYB6, Small inducible cytokine subfamily B (Cys X Cys) member 5 (epithelial derived neutrophil activating peptide 78), Small inducible cytokine subfamily B, member 15, Small-inducible cytokine B5, chemokine (C-X-C motif) ligand 5, neutrophil-activating protein 78
- sELISA
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Sandwich ELISA - Rat CXCL5 ELISA Kit (AB100775)
Representative standard curve using ab100775
Reactivity data
Product details
Abcam's CXCL5 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat CXCL5 cell lysate and tissue lysate.
This assay employs an antibody specific for Rat CXCL5 coated on a 96- well plate. Standards and samples are pipetted into the wells and CXCL5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat CXCL5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Biological function summary
CXCL5 affects inflammation and the immune response by modulating neutrophil recruitment to sites of infection or injury. It is a part of the larger network of chemokines that direct leukocyte movement throughout the body. While CXCL5 does not form a complex in a traditional sense it does interact dynamically with CXCR2 receptor to exert its effects. These interactions are pivotal to the protein’s role in orchestrating the immune system’s response to diverse stimuli.
Pathways
CXCL5 plays a part in the chemokine signaling pathway and the inflammatory response pathway. These pathways are important in mediating immune surveillance and inflammation. CXCL5 through these pathways has a strong connection to other CXC family proteins like CXCL1 and the receptor CXCR2 which allows for a coordinated response during immune challenges and recovery processes.
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