Rat CXCL7 ELISA Kit (PPBP) is a Sandwich (quantitative) ELISA kit for the measurement of Rat CXCL7 (PPBP) in Rat in Plasma, Cell culture supernatant, Serum samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 68.59 - 50000 pg/mL
- Sensitivity
- < 80 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Alternative names
Beta thromboglobulin, Beta-TG, C-X-C motif chemokine 7, CTAP 3, CTAP-III, CTAP-III(1-81), CXC chemokine ligand 7, CXCL7_HUMAN, Chemokine (C X C motif) ligand 7, Connective tissue activating peptide III, LA-PF4, LDGF, Leukocyte-derived growth factor, Low-affinity platelet factor IV, MDGF, Macrophage-derived growth factor, NAP-2, NAP-2(1-63), NAP-2(1-66), NAP-2(73), NAP-2(74), Neutrophil activating peptide 2, Neutrophil-activating peptide 2(1-63), PBP, PPBP, Platelet basic protein, Pro platelet basic protein, Pro platelet basic protein (chemokine (C-X-C motif) ligand 7), SCYB7, Small inducible cytokine subfamily B member 7, Small-inducible cytokine B7, TC2, TGB, TGB1, THBGB, THBGB1, Thrombocidin 1, Thrombocidin 2, Thromboglobulin, beta-1
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Rat CXCL7 ELISA Kit (PPBP) is a Sandwich (quantitative) ELISA kit for the measurement of Rat CXCL7 (PPBP) in Rat in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 68.59 - 50000 pg/mL
- Assay Platform
- Microplate
- Sensitivity
- < 80 pg/mL
- General recovery
- > 100%
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 115.5 | Range 104 - 124 % |
Sample type Serum | Average % = 119.2 | Range 109 - 129 % |
Sample type Plasma | Average % = 116.3 | Range 93 - 132 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Abcam's CXCL7 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat CXCL7 in serum, plasma, cell culture supernatants.
This assay employs an antibody specific for Rat CXCL7 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat CXCL7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The target CXCL7 also known as PBP PBP-172 or PPBP is a chemokine with a molecular mass of around 11 kDa. It is a platelet basic protein (PBP) belonging to the family of CXC chemokines. This protein is extensively expressed in megakaryocytes and stored in the alpha-granules of platelets. Upon platelet activation CXCL7 is released and becomes a potent attractant and activator of neutrophils. It travels through the bloodstream and impacts extracellular signaling processes.
- Biological function summary
The chemokine plays a significant role in the regulation of inflammation. CXCL7 is not only an individual entity but can form complexes with glycosaminoglycans on cell surfaces enhancing its functionality. This interaction helps in modulating immune responses by recruiting neutrophils and monocytes to sites of injury or infection. The chemokine contributes to wound healing and tissue repair through its involvement in the inflammatory phase.
- Pathways
CXCL7 is involved in the chemokine signaling pathway and is an important mediator in the immune system's response to infections. It is related to other chemokine family proteins like CXCL8 (IL-8) which share similar pathways in inflammatory responses. These proteins interact with specific receptors on leukocytes inducing changes in the cytoskeleton and promoting chemotaxis which is the directed movement towards chemical stimuli.
- Associated diseases and disorders
CXCL7 is associated with various inflammatory conditions and has been implicated in atherosclerosis and rheumatoid arthritis. In these conditions elevated levels of this chemokine correlate with increased recruitment and activation of neutrophils exacerbating inflammation. CXCL7 also shares interactions with other proteins such as CXCL1 affecting the progression and severity of these disorders. The understanding of CXCL7's role in such pathologies offers potential therapeutic avenues for intervention.
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1 product image
Sandwich ELISA - Rat CXCL7 ELISA Kit (PPBP) (ab100781)
Representative standard curve using ab100781
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Product protocols
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