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AB100781

Rat CXCL7 ELISA Kit (PPBP)

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Rat CXCL7 ELISA Kit (PPBP) is a Sandwich ELISA for the measurement of Rat CXCL7 (PPBP) in Rat in Biofluids, Cell Culture Media samples.
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Sandwich ELISA - Rat CXCL7 ELISA Kit (PPBP) (AB100781)
  • sELISA

Supplier Data

Sandwich ELISA - Rat CXCL7 ELISA Kit (PPBP) (AB100781)

Representative standard curve using ab100781

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 80 pg/mL

Range

68.59 - 50000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's CXCL7 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat CXCL7 in serum, plasma, cell culture supernatants.

This assay employs an antibody specific for Rat CXCL7 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat CXCL7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "104 - 124 %", "average": "= 115.5" }, { "sample": "Serum", "range": "109 - 129 %", "average": "= 119.2" }, { "sample": "Plasma", "range": "93 - 132 %", "average": "= 116.3" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100781-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB100781-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100781-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Rat CXCL7", "number":"AB100781-CMP05", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100781-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"CXCL7 Microplate (12 x 8 wells)", "number":"AB100781-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB100781-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100781-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Rat CXCL7 Standard (lyophilized)", "number":"AB100781-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target CXCL7 also known as PBP PBP-172 or PPBP is a chemokine with a molecular mass of around 11 kDa. It is a platelet basic protein (PBP) belonging to the family of CXC chemokines. This protein is extensively expressed in megakaryocytes and stored in the alpha-granules of platelets. Upon platelet activation CXCL7 is released and becomes a potent attractant and activator of neutrophils. It travels through the bloodstream and impacts extracellular signaling processes.
Biological function summary

The chemokine plays a significant role in the regulation of inflammation. CXCL7 is not only an individual entity but can form complexes with glycosaminoglycans on cell surfaces enhancing its functionality. This interaction helps in modulating immune responses by recruiting neutrophils and monocytes to sites of injury or infection. The chemokine contributes to wound healing and tissue repair through its involvement in the inflammatory phase.

Pathways

CXCL7 is involved in the chemokine signaling pathway and is an important mediator in the immune system's response to infections. It is related to other chemokine family proteins like CXCL8 (IL-8) which share similar pathways in inflammatory responses. These proteins interact with specific receptors on leukocytes inducing changes in the cytoskeleton and promoting chemotaxis which is the directed movement towards chemical stimuli.

CXCL7 is associated with various inflammatory conditions and has been implicated in atherosclerosis and rheumatoid arthritis. In these conditions elevated levels of this chemokine correlate with increased recruitment and activation of neutrophils exacerbating inflammation. CXCL7 also shares interactions with other proteins such as CXCL1 affecting the progression and severity of these disorders. The understanding of CXCL7's role in such pathologies offers potential therapeutic avenues for intervention.
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Product protocols

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Target data

See full target information CXCL7/PBP
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websiteProtocolBooklet
en
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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