Rat Cystatin C ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Rat Cystatin C in Tissue Extracts, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
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- Current issues in molecular biology 45:1183-11962023Myrrh Essential Oil Mitigates Renal Ischemia/Reperfusion-Induced Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNancy S YounisPubMed 36826023
- BioImpacts : BI 12:439-4482022Reduced inflammation following human endometrial stromal/stem cell injection into male Wistar rats with cisplatin-induced acute kidney injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHadis Zeinali,Mahnaz Azarnia,Peyman Keyhanvar,Reza Moghadasali,Somayeh Ebrahimi-Barough,Majid Marandi-KouchakiPubMed 36381637
- International journal of molecular sciences 23:2022Treatment of Chronic Kidney Disease with Extracellular Vesicles from Mesenchymal Stem Cells and CD133 Expanded Cells: A Comparative Preclinical Analysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDayane Mayumi Miyasaki,Alexandra Cristina Senegaglia,Sérgio Adriane Bezerra de Moura,Amanda Leitolis,Luiz Guilherme Achcar Capriglione,Letícia Fracaro,Lidiane Maria Boldrini Leite,Paulo Henrique Utumi,Felipe Yukio Ishikawa Fragoso,Fernando Meyer,Alejandro Correa,Paulo Roberto Slud BrofmanPubMed 35269664
- Ulusal travma ve acil cerrahi dergisi = Turkish journal of trauma & emergency surgery : TJTES 28:1-72021Evaluation of renal function in rats with moderate and mild brain trauma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesŞebnem Tekin Neijmann,Alev Kural,Nurten Sever,Halil Dogan,Sezgin SarıkayaPubMed 34967428
- TheScientificWorldJournal 2021:13933722021Treadmill Exercise Training Ameliorates Functional and Structural Age-Associated Kidney Changes in Male Albino Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHeba R Salem,Manar A FariedPubMed 34887703
- Environmental science and pollution research international 28:39680-396912021Evaluation of acrylamide exposure in pregnant Wistar rats as a risk of developing renal disease in their litters.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdeoye Oyewole Idris,Quadri Kunle Alabi,Mary Funmilayo Ologe,Waheed Akanni Oluogun,Marijke Haas Jimoh Akanbi,Ezekiel Olugbenga IwalewaPubMed 33763836
- Environmental toxicology 36:451-4592020Amphora algae with low-level ionizing radiation exposure ameliorate D-galactosamine-induced inflammatory impairment in rat kidney.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSawsan M El-Sonbaty,Fatma S M Moawed,Mustafa M M ElbakryPubMed 33107697
- Journal of cellular biochemistry 120:3822-38322018Protective effect of N-(p-amylcinnamoyl) anthranilic acid, phospholipase A enzyme inhibitor, and transient receptor potential melastatin-2 channel blocker against renal ischemia-reperfusion injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMurat Çakır,Suat Tekin,Aslı Taşlıdere,Pınar Çakan,Halil Düzova,Cemile Ceren GülPubMed 30259992
- Physiological genomics 48:950-9602016Cigarette smoking causes epigenetic changes associated with cardiorenal fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristopher A Drummond,Laura E Crotty Alexander,Steven T Haller,Xiaoming Fan,Jeffrey X Xie,David J Kennedy,Jiang Liu,Yanling Yan,Dawn-Alita Hernandez,Denzil P Mathew,Christopher J Cooper,Joseph I Shapiro,Jiang TianPubMed 27789733
Key facts
- Detection method
Colorimetric
- Sample types
Tissue Extracts, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Rat
- Range
23.4 - 1500 pg/mL
- Assay time
1h 30m
- Sensitivity
= 11.72 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. Known to inhibit cathepsin B, H, and L.
Alternative names
Cystatin-C, Cystatin-3, Cst3
What's included?
Recommended products
Rat Cystatin C ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Rat Cystatin C in Tissue Extracts, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
Key facts
- Detection method
Colorimetric
- Sample types
Tissue Extracts, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Rat
- Range
23.4 - 1500 pg/mL
- Assay time
1h 30m
- Assay Platform
Microplate
- Sensitivity
= 11.72 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Cystatin C | n 8 | mean - | SD - | C.V. 2.95 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Cystatin C | n 3 | mean - | SD - | C.V. 3.52 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 113 | Range 111 - 114 % |
Sample type EDTA Plasma | Average % = 115 | Range 108 - 119 % |
Sample type Citrate plasma | Average % = 112 | Range 109 - 116 % |
Sample type Cell culture media | Average % = 110 | Range 107 - 116 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Rat Cystatin C ELISA Kit (ab201281) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cystatin C protein in cell culture supernatant, cit plasma, edta plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat Cystatin C with 11.72 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Cystatin C, which belongs to the type II cystatin gene family, is a potent inhibitor of lysosomal proteinases. It is expressed in many organs of the body, and is an important extracellular inhibitor of cysteine proteases. As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Sandwich ELISA - Rat Cystatin C ELISA Kit (ab201281)
Rat Cystatin C standard curve comparison data.
Standard curve comparison between rat Cystatin C SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.
Sandwich ELISA - Rat Cystatin C ELISA Kit (ab201281)
Example of Cystatin C standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Rat Cystatin C ELISA Kit (ab201281)
Titration of rat serum, citrate plasma, and EDTA plasma within the working range of the assay.
The 1X dilution was 0.2% for serum, and 0.4% for citrate and EDTA plasma. Interpolated data values (mean +/- SD, n = 2) are graphed.
Sandwich ELISA - Rat Cystatin C ELISA Kit (ab201281)
Titration of rat Cystatin C expression in brain and heart tissue extracts.
The 1X dilution for the brain extract was an extract load of 15.6 μg/mL, and a load of 125 μg/mL for the heart extract.
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Product protocols
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