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AB201281

Rat Cystatin C ELISA Kit

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(10 Publications)

Rat Cystatin C ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat Cystatin C with a sensitivity of 25.58 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

View Alternative Names

Cystatin-C, Cystatin-3, Cst3

5 Images
Sandwich ELISA - Rat Cystatin C ELISA Kit (AB201281)
  • sELISA

Supplier Data

Sandwich ELISA - Rat Cystatin C ELISA Kit (AB201281)

Representative image of Rat Cystatin C ELISA Kit (ab201281)

Components shown from left to right :

- Sample diluent NS

- 10X Wash Buffer PT (ab206977)

- 10X Rat Cystatin C Detector Antibody

- Rat Cystatin C Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Rat Cystatin C Capture Antibody (Lyophilised)

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent CP2

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Sandwich ELISA - Rat Cystatin C ELISA Kit (AB201281)
  • sELISA

Supplier Data

Sandwich ELISA - Rat Cystatin C ELISA Kit (AB201281)

Example of Cystatin C standard curve in 1X Cell Extraction Buffer PTR.

The Cystatin C standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Rat Cystatin C ELISA Kit (AB201281)
  • sELISA

Supplier Data

Sandwich ELISA - Rat Cystatin C ELISA Kit (AB201281)

Titration of rat serum, citrate plasma, and EDTA plasma within the working range of the assay.

The 1X dilution was 0.2% for serum, and 0.4% for citrate and EDTA plasma. Interpolated data values (mean +/- SD, n = 2) are graphed.

Sandwich ELISA - Rat Cystatin C ELISA Kit (AB201281)
  • sELISA

Supplier Data

Sandwich ELISA - Rat Cystatin C ELISA Kit (AB201281)

Example of Cystatin C standard curve in Sample Diluent NS.

The Cystatin C standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Rat Cystatin C ELISA Kit (AB201281)
  • sELISA

Supplier Data

Sandwich ELISA - Rat Cystatin C ELISA Kit (AB201281)

Titration of rat Cystatin C expression in brain and heart tissue extracts.

The 1X dilution for the brain extract was an extract load of 15.6 µg/mL, and a load of 125 µg/mL for the heart extract.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 28.8 pg/mL

Range

93.8 - 1500 pg/mL

Assay time

1h 30m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Rat Cystatin C ELISA Kit (ab201281) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cystatin C protein in cell culture supernatant, cit plasma, edta plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat Cystatin C with 25.58 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Cystatin C, which belongs to the type II cystatin gene family, is a potent inhibitor of lysosomal proteinases. It is expressed in many organs of the body, and is an important extracellular inhibitor of cysteine proteases. As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Cystatin C", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.52" }, { "reproducibilityType": "Intra", "sample": "Cystatin C", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.95" } ]
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Recovery

[ { "sample": "Serum", "range": "111 - 114 %", "average": "= 113" }, { "sample": "EDTA Plasma", "range": "108 - 119 %", "average": "= 115" }, { "sample": "Citrate plasma", "range": "109 - 116 %", "average": "= 112" }, { "sample": "Cell culture media", "range": "107 - 116 %", "average": "= 110" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab201281-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB201281-CMP05", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB201281-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CP2", "number":"AB201281-CMP13", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB201281-CMP12", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Rat Cystatin C Capture Antibody (lyophilized)", "number":"AB201281-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rat Cystatin C Lyophilized Recombinant Protein", "number":"AB201281-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat Cystatin C Detector Antibody", "number":"AB201281-CMP10", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB201281-CMP07", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB201281-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB201281-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB201281-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB201281-CMP11", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. Known to inhibit cathepsin B, H, and L.
See full target information Cst3
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Basic & clinical pharmacology & toxicology 136:e14103 PubMed39520247

2024

sGC stimulator (BAY 41-8543) combined with PDE9 inhibitor (BAY 73-6691) reduces renal fibrosis in 5/6 nephrectomized rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Chen,Denis Delić,Yvonne Liu,Yaochen Cao,Zeyu Zhang,Hongwei Wu,Mohamed M S Gaballa,Thomas Klein,Saban Elitok,Bernhard K Krämer,Berthold Hocher

Current issues in molecular biology 45:1183-1196 PubMed36826023

2023

Myrrh Essential Oil Mitigates Renal Ischemia/Reperfusion-Induced Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Nancy S Younis

BioImpacts : BI 12:439-448 PubMed36381637

2022

Reduced inflammation following human endometrial stromal/stem cell injection into male Wistar rats with cisplatin-induced acute kidney injury.

Applications

Unspecified application

Species

Unspecified reactive species

Hadis Zeinali,Mahnaz Azarnia,Peyman Keyhanvar,Reza Moghadasali,Somayeh Ebrahimi-Barough,Majid Marandi-Kouchaki

International journal of molecular sciences 23: PubMed35269664

2022

Treatment of Chronic Kidney Disease with Extracellular Vesicles from Mesenchymal Stem Cells and CD133 Expanded Cells: A Comparative Preclinical Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Dayane Mayumi Miyasaki,Alexandra Cristina Senegaglia,Sérgio Adriane Bezerra de Moura,Amanda Leitolis,Luiz Guilherme Achcar Capriglione,Letícia Fracaro,Lidiane Maria Boldrini Leite,Paulo Henrique Utumi,Felipe Yukio Ishikawa Fragoso,Fernando Meyer,Alejandro Correa,Paulo Roberto Slud Brofman

Ulusal travma ve acil cerrahi dergisi = Turkish journal of trauma & emergency surgery : TJTES 28:1-7 PubMed34967428

2021

Evaluation of renal function in rats with moderate and mild brain trauma.

Applications

Unspecified application

Species

Unspecified reactive species

Şebnem Tekin Neijmann,Alev Kural,Nurten Sever,Halil Dogan,Sezgin Sarıkaya

TheScientificWorldJournal 2021:1393372 PubMed34887703

2021

Treadmill Exercise Training Ameliorates Functional and Structural Age-Associated Kidney Changes in Male Albino Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Heba R Salem,Manar A Faried

Environmental science and pollution research international 28:39680-39691 PubMed33763836

2021

Evaluation of acrylamide exposure in pregnant Wistar rats as a risk of developing renal disease in their litters.

Applications

Unspecified application

Species

Unspecified reactive species

Adeoye Oyewole Idris,Quadri Kunle Alabi,Mary Funmilayo Ologe,Waheed Akanni Oluogun,Marijke Haas Jimoh Akanbi,Ezekiel Olugbenga Iwalewa

Environmental toxicology 36:451-459 PubMed33107697

2020

Amphora algae with low-level ionizing radiation exposure ameliorate D-galactosamine-induced inflammatory impairment in rat kidney.

Applications

Unspecified application

Species

Unspecified reactive species

Sawsan M El-Sonbaty,Fatma S M Moawed,Mustafa M M Elbakry

Journal of cellular biochemistry 120:3822-3832 PubMed30259992

2018

Protective effect of N-(p-amylcinnamoyl) anthranilic acid, phospholipase A enzyme inhibitor, and transient receptor potential melastatin-2 channel blocker against renal ischemia-reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Murat Çakır,Suat Tekin,Aslı Taşlıdere,Pınar Çakan,Halil Düzova,Cemile Ceren Gül

Physiological genomics 48:950-960 PubMed27789733

2016

Cigarette smoking causes epigenetic changes associated with cardiorenal fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher A Drummond,Laura E Crotty Alexander,Steven T Haller,Xiaoming Fan,Jeffrey X Xie,David J Kennedy,Jiang Liu,Yanling Yan,Dawn-Alita Hernandez,Denzil P Mathew,Christopher J Cooper,Joseph I Shapiro,Jiang Tian
View all publications
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