AB202413

Rat E-Cadherin ELISA Kit

Recombinant
SimpleStep
What is this?

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(5 Publications)

Rat E-Cadherin ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat E-Cadherin with a sensitivity of 17 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD324, Cadherin-1, Epithelial cadherin, Uvomorulin, E-cadherin, Cdh1

2 Images

Sandwich ELISA - Rat E-Cadherin ELISA Kit (AB202413)

sELISA

Supplier Data

Sandwich ELISA - Rat E-Cadherin ELISA Kit (AB202413)

Example of E-Cadherin standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Rat E-Cadherin ELISA Kit (AB202413)

Interpolated concentrations of E-Cadherin in rat serum, plasma (citrate), and urine.

The concentrations of E-Cadherin were measured in duplicate and interpolated from the E-Cadherin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean E-Cadherin concentration was determined to be 983.9 ng/mL in serum, 620.7 ng/mL in plasma (Citrate), and 1,771 ng/mL in urine.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum, Urine

Reacts with

Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 17 pg/mL

Range

156.25 - 10000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Rat E-Cadherin ELISA Kit (ab202413) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of E-Cadherin protein in cell culture supernatant, serum, urine, cit plasma, edta plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat E-Cadherin with 17 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

E-Cadherin is a member of the Cadherin family of calcium-dependent cell adhesion proteins. E-Cadherin is a single-pass transmembrane protein on the plasma membrane. The extracellular portion contains 5 cadherin domains that bind calcium and form homodimeric interactions. E-Cadherin is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.6" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.2" } ]

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Recovery

[ { "sample": "Serum", "range": "111 - 119 %", "average": "= 116" }, { "sample": "Urine", "range": "101 - 113 %", "average": "= 107" }, { "sample": "Citrate plasma", "range": "113 - 117 %", "average": "= 115" }, { "sample": "1X Cell Extraction Buffer PTR", "range": "85 - 88 %", "average": "= 86" }, { "sample": "Fetal Bovine Serum", "range": "94 - 96 %", "average": "= 96" }, { "sample": "serum free media", "range": "95 - 98 %", "average": "= 96" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab202413-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB202413-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB202413-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB202413-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB202413-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB202413-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB202413-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BC", "number":"AB202413-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat E-Cadherin Capture Antibody", "number":"AB202413-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rat E-Cadherin Lyophilized Recombinant Protein", "number":"AB202413-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat E-Cadherin Detector Antibody", "number":"AB202413-CMP08", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

E-Cadherin sometimes called CDH1 or Cadherin-1 is a protein that plays a role in cell-to-cell adhesion. This transmembrane protein has a molecular weight of approximately 120 kDa. E-Cadherin is mainly expressed in epithelial tissues of various organs including the skin and gut. Its adhesive function is made possible by its extracellular domain that facilitates homophilic binding between cells contributing to the maintenance of tissue architecture and cellular integrity.

Biological function summary

E-Cadherin participates in establishing and maintaining adherens junctions which are vital for tissue structure. E-Cadherin operates as a core component of the cadherin-catenin complex which links the protein to the actin cytoskeleton. Through this linkage E-Cadherin plays an important role in signaling pathways that influence cellular growth and differentiation. The protein's ability to mediate intercellular connections also regulates cellular motility and supports basic aspects of cell behavior in epithelial tissues.

Pathways

E-Cadherin influences both the Wnt signaling pathway and the epithelial-to-mesenchymal transition (EMT). Within the Wnt signaling pathway E-Cadherin partners with β-catenin a significant player in transcription regulation and cell signaling. Disruption in E-Cadherin's adhesive functionality can lead to increased β-catenin availability affecting downstream transcriptional control. In the EMT process E-Cadherin loss characterizes an important step in which cells gain migratory and invasive properties typically seen during metastasis in cancer progression.

E-Cadherin's role is prominent in cancer particularly in the context of gastric and breast cancers. Mutations or altered expression of E-Cadherin lead to diminished cell adhesion promoting tumorigenesis and metastatic spread. In gastric cancer for instance mutated E-Cadherin often associates with loss of epithelial function facilitating cancer cell dissemination. Additionally its loss or dysfunction may correlate with proteins such as β-catenin further impacting cancer progression and pathology.

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Product protocols

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Target data

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Promotes organization of radial actin fiber structure and cellular response to contractile forces, via its interaction with AMOTL2 which facilitates anchoring of radial actin fibers to CDH1 junction complexes at the cell membrane (By similarity). Plays a role in the early stages of desmosome cell-cell junction formation via facilitating the recruitment of DSG2 and DSP to desmosome plaques (By similarity). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production (By similarity).

See full target information Cdh1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of enzyme inhibition and medicinal chemistry 38:2185761 PubMed36912230

2023

WRH-2412 alleviates the progression of hepatocellular carcinoma through regulation of TGF-β/β-catenin/α-SMA pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammed A F Elewa,Wagdy M Eldehna,Ahmed M E Hamdan,Samraa H Abd El-Kawi,Asmaa M El-Kalaawy,Taghreed A Majrashi,Reham F Barghash,Hatem A Abdel-Aziz,Khalid S Hashem,Mohammed M H Al-Gayyar

Biomaterials research 27:2 PubMed36635718

2023

A simple method to isolate structurally and chemically intact brain vascular basement membrane for neural regeneration following traumatic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Wanqing Ji,Zhiru Wu,Jiaming Wen,Hengxin Tang,Zhuopeng Chen,Bo Xue,Zhenming Tian,Yueyang Ba,Ning Zhang,Xuejun Wen,Bo Hou

Molecular medicine reports 21:615-622 PubMed31974597

2020

Effect of the alteration of Tribbles homologue 3 expression on epithelial‑mesenchymal transition of transforming growth factor β1‑induced mouse alveolar epithelial cells through the Wnt/β‑catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wencheng Yu,Liyun Mi,Feifei Wang

American journal of physiology. Endocrinology and 317:E686-E698 PubMed31361542

2019

IGF-1/IGF-1R blockade ameliorates diabetic kidney disease through normalizing Snail1 expression in a mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Dong,Jiali Yu,Funxun Yu,Song Yang,Qi Qian,Yan Zha

Scientific reports 8:14327 PubMed30254303

2018

Anti-fibrotic impact of Carvedilol in a CCl-4 model of liver fibrosis via serum microRNA-200a/SMAD7 enhancement to bridle TGF-β1/EMT track.

Applications

Unspecified application

Species

Unspecified reactive species

Sara A El-Wakeel,Rania M Rahmo,Hanan S El-Abhar

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rat E-Cadherin ELISA Kit

Sandwich ELISA - Rat E-Cadherin ELISA Kit (AB202413)

Sandwich ELISA - Rat E-Cadherin ELISA Kit (AB202413)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise