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AB193734

Rat EGF ELISA Kit

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Rat EGF ELISA Kit is a Sandwich ELISA for the measurement of Rat EGF in Rat in Biofluids, Cell Culture Media samples.

View Alternative Names

Pro-epidermal growth factor, EGF, Egf

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ELISA - Rat EGF ELISA Kit (AB193734)
  • ELISA

Supplier Data

ELISA - Rat EGF ELISA Kit (AB193734)

Standard curve - diluent A.

Representative Standard curve - using ab193734 Rat EGF standards in diluent A

ELISA - Rat EGF ELISA Kit (AB193734)
  • ELISA

Supplier Data

ELISA - Rat EGF ELISA Kit (AB193734)

Standard curve - diluent B.

Representative Standard curve - using ab193734 Rat EGF standards in diluent B

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.8 pg/mL

Range

0.8 - 200 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Primary Antibodies

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Primary Antibodies

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Anti-Ki67 antibody

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's EGF Rat ELISA Kit (ab193734) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat EGF in sera, plasma and cell culture supernatants.

This assay employs an antibody specific for rat EGF coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures EGF present in the samples. The wells are washed and biotinylated anti-rat EGF antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of EGF bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Cell culture media", "range": "81 - 113 %", "average": "= 98.21" }, { "sample": "Plasma", "range": "69 - 135 %", "average": "= 104.6" }, { "sample": "Serum", "range": "68 - 110 %", "average": "= 88.98" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab193734-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"rat EGF standards (lyophilized)", "number":"AB193734-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Rat EGF detection antibody", "number":"AB193734-CMP05", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB193734-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pre-coated rat EGF Microplate (12 strips x 8 wells)", "number":"AB193734-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB193734-CMP07", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB193734-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB193734-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB193734-CMP04", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB193734-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Epidermal Growth Factor (EGF) also known as urogastrone is a single polypeptide that facilitates cell growth proliferation and differentiation by binding to the Epidermal Growth Factor Receptor (EGFR). The EGF protein is characterized by a relatively small molecular weight of approximately 6.4 kDa. It is expressed in various tissues including the kidney submandibular glands and Brunner's glands in the mouse and other species. The presence of anti-EGF antibodies can further enhance the understanding of its mechanical actions and functions in different organisms.
Biological function summary

The epidermal growth factor plays a pivotal role in the regulation of cell signaling. It functions outside a complex interacting mainly with EGFR located on the cell surface. Upon ligand binding EGF induces the receptor dimerization leading to autophosphorylation which activates intrinsic intracellular signaling cascades. This activation is critical for cellular responses such as DNA synthesis and the progression of the cell cycle. EGF recombinant proteins such as HEGF are utilized in laboratories to examine its biological activity meticulously.

Pathways

EGF engages in key cellular pathways that mediate various essential functions. It significantly activates the MAPK and PI3K-AKT signaling pathways which govern processes such as cell division and survival. These pathways require the participation of several other proteins including Ras Raf and PDK1 which help modulate their downstream effects. The interplay of EGF with associated proteins highlights its importance in maintaining proper signaling dynamics necessary for healthy cellular function. EGF ELISA kits as well as EGF ELISAs are tools developed for robust analysis of its pathway interactions in research and clinical settings.

Altered EGF signaling is associated with increased risk of certain cancers and inflammatory diseases. Overexpression or mutations in the EGF-EGFR axis can lead to the progression of carcinomas such as those found in the lung and breast as well as disorders like psoriasis. These aberrant signaling events are often linked with other proteins such as HER2 and VEGF which can exacerbate disease severity. The study and detection of EGF products therefore offer significant insights into both the progression and potential therapeutic targeting of these conditions.
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Product protocols

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Target data

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6 (By similarity).
See full target information Egf
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websiteProtocolBooklet
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Product promise

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