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AB274398

Rat Erythropoietin ELISA Kit (EPO)

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(1 Publication)

Rat Erythropoietin ELISA Kit (EPO) is a single-wash 90-min Simplestep used to quantify Rat Erythropoietin (EPO) with a sensitivity of 2.8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Erythropoietin, Epo

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ELISA - Rat Erythropoietin ELISA Kit (EPO) (AB274398)
  • ELISA

Supplier Data

ELISA - Rat Erythropoietin ELISA Kit (EPO) (AB274398)
Sandwich ELISA - Rat Erythropoietin ELISA Kit (EPO) (AB274398)
  • sELISA

Supplier Data

Sandwich ELISA - Rat Erythropoietin ELISA Kit (EPO) (AB274398)

Example of rat Erythropoietin standard curve in Sample Diluent NS.

The Erythropoietin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Rat Erythropoietin ELISA Kit (EPO) (AB274398)
  • sELISA

Supplier Data

Sandwich ELISA - Rat Erythropoietin ELISA Kit (EPO) (AB274398)

Interpolated concentrations of native Erythropoietin in Sprague Dawley (SD) rat serum and plasma samples.

The concentrations of Erythropoietin were measured in duplicates, interpolated from the Erythropoietin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100%, plasma (citrate) 100%, plasma (EDTA) 100% and plasma (heparin) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Erythropoietin concentration was determined to be 135 pg/mL in neat serum, 98 pg/mL in neat plasma (citrate) , 45 pg/mL in neat plasma (EDTA) and 73 pg/mL in neat plasma (heparin).

Sandwich ELISA - Rat Erythropoietin ELISA Kit (EPO) (AB274398)
  • sELISA

Supplier Data

Sandwich ELISA - Rat Erythropoietin ELISA Kit (EPO) (AB274398)

Interpolated concentrations of native Erythropoietin in Wistar and Fischer rat serum samples.

The concentrations of Erythropoietin were measured in duplicates, interpolated from the Erythropoietin standard curves and corrected for sample dilution. Undiluted samples are as follows : Wistar serum 100% and Fischer serum 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Erythropoietin concentration was determined to be 108 pg/mL in neat Wistar rat serum and 64 pg/mL in neat Fischer rat serum.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 2.8 pg/mL

Range

11.72 - 750 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Rat Erythropoietin ELISA Kit (EPO) ab274398 is a rapid single-wash 90-min Sandwich ELISA to measure Rat Erythropoietin (EPO)in cell culture media, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 2.8 pg/mL.

How the assay works

Rat Erythropoietin (EPO)SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Rat Erythropoietin ELISA Kit (EPO) ab274398 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Erythropoietin is the principal hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Erythropoietin binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3. Erythropoietin is a secreted protein produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals. Human erythropoietin is used for the treatment of anemia. Variations affecting the human EPO gene are associated with microvascular complications of diabetes 2. Rat Erythropoietin has 94% amino acids sequence identity to mouse Erythropoietin, and 83% identity to the human Erythropoietin.

The standard is affinity purified.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.5" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.2" } ]
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Recovery

[ { "sample": "Serum", "range": "101 - 109 %", "average": "= 105" }, { "sample": "EDTA Plasma", "range": "88 - 97 %", "average": "= 92" }, { "sample": "Cell culture media", "range": "92 - 95 %", "average": "= 93" }, { "sample": "Heparin Plasma", "range": "81 - 91 %", "average": "= 87" }, { "sample": "Citrate plasma", "range": "81 - 96 %", "average": "= 89" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Erythropoietin often called EPO or erythropoietin protein is a glycoprotein hormone with a mass of approximately 30.4 kDa. It plays a mechanical role in the production of red blood cells (erythropoiesis) by acting as a signaling molecule that binds the erythropoietin receptor on erythroid progenitor cells in the bone marrow. Erythropoietin is mainly expressed in the kidneys with some expression in the liver. It controls the proliferation and differentiation of precursor cells into mature red blood cells which are responsible for oxygen transport.
Biological function summary

Erythropoietin regulates blood oxygen levels by stimulating erythrocyte production. It is not part of a larger complex but interacts directly with the erythropoietin receptor activating intracellular pathways necessary for erythroid progenitor cell survival and proliferation. Eyrthropoietin production increases in response to low oxygen levels in tissues ensuring adequate oxygen delivery throughout the body by increasing red blood cell count.

Pathways

Erythropoietin predominantly influences the JAK-STAT signaling pathway. Upon binding to its receptor on erythroid progenitors it activates this pathway leading to gene expression changes that promote survival and proliferation of these cells. This pathway is also involved in various immune responses and cell growth processes. Erythropoietin signaling has a regulatory relationship with other growth factors like thrombopoietin which is another hematopoietic hormone.

Erythropoietin is important in the treatment of anemia especially anemia associated with chronic kidney disease where natural EPO production decreases. Abnormal levels of erythropoietin are associated with conditions such as polycythemia vera a disorder characterized by excessive red blood cell production. EPO levels can be affected by production defects in the kidneys linking it to proteins like the von Hippel-Lindau tumor suppressor which regulates erythropoietin transcription under low oxygen conditions.
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Product protocols

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Target data

Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3.
See full target information Epo
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 13:876573 PubMed35517793

2022

Dynamics of Erythroferrone Response to Erythropoietin in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Xu,Raymond S M Wong,Wojciech Krzyzanski,Xiaoyu Yan
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websiteProtocolBooklet
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