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AB326897

Rat Erythropoietin (EPO) ELISA Kit, Chemiluminescent

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Rat Erythropoietin (EPO) ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Rat Erythropoietin (EPO) with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 3.43 - 7500 pg/ml with a sensitivity of 0.55 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

View Alternative Names

Erythropoietin, Epo

4 Images
Sandwich ELISA - Rat Erythropoietin (EPO) ELISA Kit, Chemiluminescent (AB326897)
  • sELISA

Lab

Sandwich ELISA - Rat Erythropoietin (EPO) ELISA Kit, Chemiluminescent (AB326897)

Dynamic range comparison between the Chemiluminescent Rat Erythropoietin SimpleStep ELISA, colorimetric Rat Erythropoietin SimpleStep ELISA (ab274398). The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Rat Erythropoietin (EPO) ELISA Kit, Chemiluminescent (AB326897)
  • sELISA

Lab

Sandwich ELISA - Rat Erythropoietin (EPO) ELISA Kit, Chemiluminescent (AB326897)

Standard curve comparison between the Chemiluminescent Rat Erythropoietin SimpleStep ELISA and colorimetric Rat Erythropoietin SimpleStep ELISA (ab274398) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Rat Erythropoietin (EPO) ELISA Kit, Chemiluminescent (AB326897)
  • sELISA

Supplier Data

Sandwich ELISA - Rat Erythropoietin (EPO) ELISA Kit, Chemiluminescent (AB326897)

Interpolated concentrations of native Erythropoietin in Sprague Dawley (SD) rat serum and plasma samples.

The concentrations of Erythropoietin were measured in duplicates, interpolated from the Erythropoietin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100%, plasma (citrate) 100%, plasma (EDTA) 100% and plasma (heparin) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Erythropoietin concentration was determined to be 135 pg/mL in neat serum, 98 pg/mL in neat plasma (citrate) , 45 pg/mL in neat plasma (EDTA) and 73 pg/mL in neat plasma (heparin).

Sandwich ELISA - Rat Erythropoietin (EPO) ELISA Kit, Chemiluminescent (AB326897)
  • sELISA

Supplier Data

Sandwich ELISA - Rat Erythropoietin (EPO) ELISA Kit, Chemiluminescent (AB326897)

Interpolated concentrations of native Erythropoietin in Wistar and Fischer rat serum samples.

The concentrations of Erythropoietin were measured in duplicates, interpolated from the Erythropoietin standard curves and corrected for sample dilution. Undiluted samples are as follows : Wistar serum 100% and Fischer serum 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Erythropoietin concentration was determined to be 108 pg/mL in neat Wistar rat serum and 64 pg/mL in neat Fischer rat serum.

Key facts

Detection method

Luminescent

Sample types

Serum, EDTA Plasma, Cell culture media, Heparin Plasma, Citrate plasma

Reacts with

Human, Mouse, Rat, Monkey

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.55 pg/mL

Range

3.43 - 7500 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Rat Erythropoietin (EPO) ELISA Kit, Chemiluminescent (ab326897) is a rapid single-wash sandwich 55-min ELISA to measure Rat Erythropoietin (EPO) in Serum, EDTA Plasma, Cell culture media, Heparin Plasma, Citrate plasma with a sensitivity of 0.55 pg/mL.

How the assay works

Rat Erythropoietin (EPO) ELISA Kit, Chemiluminescent (ab326897) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Rat Erythropoietin (EPO) (ab326897) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-EPO antibody [EPR22931-17] - BSA and Aside free (Capture) ab272358
- Anti-EPO antibody [EPR22931-29] - BSA and Aside free (Detector) ab272359
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Precision

[ { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.2" }, { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.5" } ]
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Recovery

[ { "sample": "Serum", "range": "101 - 109 %", "average": "= 109" }, { "sample": "EDTA Plasma", "range": "88 - 97 %", "average": "= 97" }, { "sample": "Cell culture media", "range": "92 - 95 %", "average": "= 95" }, { "sample": "Heparin Plasma", "range": "81 - 91 %", "average": "= 91" }, { "sample": "Citrate plasma", "range": "81 - 96 %", "average": "= 96" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3.
See full target information Epo
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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