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Rat Ferritin ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Rat Ferritin in Rat in Plasma, Serum, Other biological fluids samples.

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Sandwich ELISA - Rat Ferritin ELISA Kit (AB157732), expandable thumbnail

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Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum, Other biological fluids

Assay type

Sandwich (quantitative)

Reactive species

Rat

Range

12.5 - 400 ng/mL

Sensitivity

= 2.7695 ng/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info
-
Notes

-

Target data

Function

Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity). Delivery to lysosomes by the cargo receptor NCOA4 for autophagic degradation and release or iron (By similarity).

Alternative names

What's included?

1 x 96 Tests
Components
100X HRP-conjugated anti-rat Ferritin antibody
1 x 150 µL
20X Wash Buffer Concentrate
1 x 50 mL
5X Diluent Concentrate
1 x 50 mL
Chromogen Substrate Solution
1 x 12 mL
Rat Ferritin Calibrator (Lyophilized)
1 x 1 Vial
Rat Ferritin ELISA Microplate
1 x 1 Unit
Stop Solution
1 x 12 mL

Recommended products

Rat Ferritin ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Rat Ferritin in Rat in Plasma, Serum, Other biological fluids samples.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum, Other biological fluids

Assay type

Sandwich (quantitative)

Reactive species

Rat

Range

12.5 - 400 ng/mL

Assay Platform

Microplate

Sensitivity

= 2.7695 ng/mL

Precision

Intra assay

Sample

Overall

n
0
mean
-
SD
-
C.V.
< 10

Inter assay

Sample

Overall

n
0
mean
-
SD
-
C.V.
< 10

Recovery

Sample specific recovery

Sample type

Serum

Average %

> 85

Range

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

Notes

Abcam's Ferritin Rat ELISA Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for the quantitative measurement of ferritin in rat biological samples.

In this assay the ferritin present in samples reacts with the anti-Ferritin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Ferritin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Ferritin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromo­genic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Ferritin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Ferritin in the test sample. The quantity of Ferritin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Rat Ferritin ELISA Kit (ab157732), expandable thumbnail

    Sandwich ELISA - Rat Ferritin ELISA Kit (ab157732)

    Representative standard curve using ab157732 Ferritin Rat ELISA Kit.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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