Rat Ferritin ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Rat Ferritin in Rat in Plasma, Serum, Other biological fluids samples.
Colorimetric
Plasma, Serum, Other biological fluids
Sandwich (quantitative)
Rat
12.5 - 400 ng/mL
= 2.7695 ng/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity). Delivery to lysosomes by the cargo receptor NCOA4 for autophagic degradation and release or iron (By similarity).
Ftl, Ftl1, Ferritin light chain 1, Ferritin L subunit 1
Rat Ferritin ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Rat Ferritin in Rat in Plasma, Serum, Other biological fluids samples.
Colorimetric
Plasma, Serum, Other biological fluids
Sandwich (quantitative)
Rat
12.5 - 400 ng/mL
Microplate
= 2.7695 ng/mL
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Sample type | Average % | Range |
---|---|---|
Sample type Serum | Average % > 85 | Range |
Blue Ice
+4°C
Multi
Please refer to protocols
Abcam's Ferritin Rat ELISA Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for the quantitative measurement of ferritin in rat biological samples.
In this assay the ferritin present in samples reacts with the anti-Ferritin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Ferritin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Ferritin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Ferritin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Ferritin in the test sample. The quantity of Ferritin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
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Representative standard curve using ab157732 Ferritin Rat ELISA Kit.
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