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AB157732

Rat Ferritin ELISA Kit

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(6 Publications)

Rat Ferritin ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Rat Ferritin in Rat in Biofluids samples.

View Alternative Names

Ftl, Ftl1, Ferritin light chain 1, Ferritin L subunit 1

1 Images
Sandwich ELISA - Rat Ferritin ELISA Kit (AB157732)
  • sELISA

Supplier Data

Sandwich ELISA - Rat Ferritin ELISA Kit (AB157732)

Representative standard curve using ab157732 Ferritin Rat ELISA Kit.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum, Other biological fluids

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 2.7695 ng/mL

Range

12.5 - 400 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Ferritin Rat ELISA Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for the quantitative measurement of ferritin in rat biological samples.

In this assay the ferritin present in samples reacts with the anti-Ferritin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Ferritin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Ferritin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromo­genic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Ferritin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Ferritin in the test sample. The quantity of Ferritin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": null, "average": "> 85" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab157732-1x96Tests", "assetComponentDetails": [ { "size":"1 x 150 µL", "name":"100X HRP-conjugated anti-rat Ferritin antibody", "number":"AB157732-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Rat Ferritin ELISA Microplate", "number":"AB157732-CMP02", "productcode":"" }, { "size":"1 x 50 mL", "name":"5X Diluent Concentrate", "number":"AB157732-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB157732-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Rat Ferritin Calibrator (Lyophilized)", "number":"AB157732-CMP01", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Wash Buffer Concentrate", "number":"AB157732-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Chromogen Substrate Solution", "number":"AB157732-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity). Delivery to lysosomes by the cargo receptor NCOA4 for autophagic degradation and release or iron (By similarity).
See full target information Ftl1
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Nanomaterials (Basel, Switzerland) 12: PubMed35407158

2022

Assessment of Systemic Toxicity, Genotoxicity, and Early Phase Hepatocarcinogenicity of Iron (III)-Tannic Acid Nanoparticles in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Chi Be Hlaing,Arpamas Chariyakornkul,Chalermchai Pilapong,Charatda Punvittayagul,Somdet Srichairatanakool,Rawiwan Wongpoomchai

Annals of palliative medicine 11:1687-1699 PubMed35016521

2022

Intravenous iron injection does not impair liver function in a rat model of cirrhosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Hye Kwon,Sukyoung Her,Seungwon Lee,Sangbin Han,Mi Sook Gwak,Gaab Soo Kim,Tae Soo Hahm,Hyun Sung Cho,Justin S Ko

Molecules (Basel, Switzerland) 26: PubMed34946518

2021

Anti-Oxidant and Anti-Inflammatory Effects of Lipopolysaccharide from against Ethanol-Induced Liver and Kidney Toxicity in Experimental Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Eman T Mehanna,Al-Shimaa A Ali,Fatma El-Shaarawy,Noha M Mesbah,Dina M Abo-Elmatty,Nora M Aborehab

Nutrients 13: PubMed33802720

2021

Iron Protein Succinylate in the Management of Iron Deficiency Anemia: A Comparative Study with Ferrous Sulphate at Low and High Therapeutic Doses.

Applications

Unspecified application

Species

Unspecified reactive species

Katia Urso,Javier Leal Martínez-Bujanda,Jaime Moscoso Del Prado

Nature communications 10:5134 PubMed31723130

2019

Renal clearable nanochelators for iron overload therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Homan Kang,Murui Han,Jie Xue,Yoonji Baek,JuOae Chang,Shuang Hu,HaYoung Nam,Min Joo Jo,Georges El Fakhri,Michael P Hutchens,Hak Soo Choi,Jonghan Kim

Scientific reports 7:46573 PubMed28440316

2017

Modest and Severe Maternal Iron Deficiency in Pregnancy are Associated with Fetal Anaemia and Organ-Specific Hypoxia in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew G Woodman,Alison S Care,Yael Mansour,Stephana J Cherak,Sareh Panahi,Ferrante S Gragasin,Stephane L Bourque
View all publications
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Primary Antibodies

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