Rat FGF-21 ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat FGF-21 with a sensitivity of 6.8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Publications
Filter by
- Acta physiologica (Oxford, England) 238:e139352023Rats lacking Ucp1 present a novel translational tool for the investigation of thermogenic adaptation during cold challenge.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJaycob D Warfel,Carrie M Elks,David S Bayless,Bolormaa Vandanmagsar,Allison C Stone,Samuel E Velasquez,Paola Olivares-Nazar,Robert C Noland,Sujoy Ghosh,Jingying Zhang,Randall L MynattPubMed 36650072
- Journal of molecular histology 52:905-9182021Elabela prevents angiotensin II-induced apoptosis and inflammation in rat aortic adventitial fibroblasts via the activation of FGF21-ACE2 signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuan-Juan Song,Mei Yang,Ying Liu,Jia-Wei Song,Xiao-Yan Liu,Ran Miao,Zhen-Zhou Zhang,Yu Liu,Yi-Fan Fan,Qian Zhang,Ying Dong,Xin-Chun Yang,Jiu-Chang ZhongPubMed 34453661
- Experimental neurology 320:1130072019Delayed recanalization at 3 days after permanent MCAO attenuates neuronal apoptosis through FGF21/FGFR1/PI3K/Caspase-3 pathway in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWen Zheng et. al.PubMed 31295445
Key facts
- Detection method
- Colorimetric
- Sample types
- Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 31.25 - 2000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 6.8 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
What's included?
Recommended products
Rat FGF-21 ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat FGF-21 with a sensitivity of 6.8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Rat
- Range
- 31.25 - 2000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 6.8 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 5 | C.V. 4 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 5.4 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 94 | Range 88 - 97 % |
Sample type EDTA Plasma | Average % = 83 | Range 81 - 85 % |
Sample type Citrate plasma | Average % = 89 | Range 84 - 92 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Rat FGF-21 ELISA Kit (ab223589) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FGF-21 protein in cell culture supernatant, cit plasma, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat FGF-21 with 6.8 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
FGF-21 is a secreted protein, a member of a large FGF gene family. FGF-21 stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression but not via SLC2A4/GLUT4 expression. FGF-21 activity probably requires the presence of KLB.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FGF21 or fibroblast growth factor 21 is an important metabolic regulator. It is a member of the fibroblast growth factor family but unlike many others FGF21 functions mainly in endocrine signaling. The molecular weight of FGF21 is approximately 22 kDa. This protein is expressed in tissues such as the liver adipose tissue and pancreas. Because of its endocrine roles FGF21 travels through the bloodstream to exert its functions on distant organs.
- Biological function summary
FGF21 influences glucose and lipid metabolism. It does not act as part of a larger protein complex. Instead it directly affects metabolic processes enhancing insulin sensitivity and lipid oxidation while reducing body weight. These actions help maintain energy balance and homeostasis making FGF21 an important player in metabolic control.
- Pathways
FGF21 plays a significant role in the insulin signaling pathway and PPARγ coactivator 1α (PGC-1α) pathway. These pathways regulate cellular energy metabolism and mitochondrial biogenesis respectively. FGF21 interacts with other molecules such as PPARα (peroxisome proliferator-activated receptor alpha) to exert its effects demonstrating its integration into broader metabolic networks. FGF21's interaction with these pathways highlights its function in energy utilization and storage.
- Associated diseases and disorders
FGF21 has connections to conditions such as obesity and type 2 diabetes. Its role in improving insulin sensitivity and promoting lipid oxidation makes it a molecule of interest in combating metabolic syndrome. FGF21 also has a relationship with hepatic proteins such as insulin receptor substrates (IRS) which play roles in liver functioning. Understanding these relationships offers insights into potential therapeutic strategies targeting metabolic disorders.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Sandwich ELISA - Rat FGF-21 ELISA Kit (ab223589)
Example of rat FGF-21 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Rat FGF-21 ELISA Kit (ab223589)
Interpolated concentrations of native FGF-21 in rat serum and plasma samples.
The concentrations of FGF-21 were measured in duplicates, interpolated from the FGF-21 standard curves and corrected for sample dilution. Undiluted samples are as follows: normal serum neat, pregnant serum 12.5%, normal plasma (citrate) neat, normal plasma (EDTA) neat. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGF-21 concentration was determined to be 580.1 pg/mL in neat normal serum, 3361 pg/mL in neat pregnant serum, 548.7 pg/mL in neat normal plasma (citrate), and 782.4 pg/mL in neat normal plasma (EDTA).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com