AB223589

Rat FGF-21 ELISA Kit

Recombinant
SimpleStep
What is this?

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(4 Publications)

Rat FGF-21 ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat FGF-21 with a sensitivity of 6.8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

2 Images

Sandwich ELISA - Rat FGF-21 ELISA Kit (AB223589)

sELISA

Supplier Data

Sandwich ELISA - Rat FGF-21 ELISA Kit (AB223589)

Interpolated concentrations of native FGF-21 in rat serum and plasma samples.

The concentrations of FGF-21 were measured in duplicates, interpolated from the FGF-21 standard curves and corrected for sample dilution. Undiluted samples are as follows : normal serum neat, pregnant serum 12.5%, normal plasma (citrate) neat, normal plasma (EDTA) neat. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGF-21 concentration was determined to be 580.1 pg/mL in neat normal serum, 3361 pg/mL in neat pregnant serum, 548.7 pg/mL in neat normal plasma (citrate), and 782.4 pg/mL in neat normal plasma (EDTA).

sELISA

Supplier Data

Sandwich ELISA - Rat FGF-21 ELISA Kit (AB223589)

Example of rat FGF-21 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 6.8 pg/mL

Range

31.25 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Rat FGF-21 ELISA Kit (ab223589) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FGF-21 protein in cell culture supernatant, cit plasma, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat FGF-21 with 6.8 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

FGF-21 is a secreted protein, a member of a large FGF gene family. FGF-21 stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression but not via SLC2A4/GLUT4 expression. FGF-21 activity probably requires the presence of KLB.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.4" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4" } ]

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Recovery

[ { "sample": "Serum", "range": "88 - 97 %", "average": "= 94" }, { "sample": "EDTA Plasma", "range": "81 - 85 %", "average": "= 83" }, { "sample": "Citrate plasma", "range": "84 - 92 %", "average": "= 89" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FGF21 or fibroblast growth factor 21 is an important metabolic regulator. It is a member of the fibroblast growth factor family but unlike many others FGF21 functions mainly in endocrine signaling. The molecular weight of FGF21 is approximately 22 kDa. This protein is expressed in tissues such as the liver adipose tissue and pancreas. Because of its endocrine roles FGF21 travels through the bloodstream to exert its functions on distant organs.

Biological function summary

FGF21 influences glucose and lipid metabolism. It does not act as part of a larger protein complex. Instead it directly affects metabolic processes enhancing insulin sensitivity and lipid oxidation while reducing body weight. These actions help maintain energy balance and homeostasis making FGF21 an important player in metabolic control.

Pathways

FGF21 plays a significant role in the insulin signaling pathway and PPARγ coactivator 1α (PGC-1α) pathway. These pathways regulate cellular energy metabolism and mitochondrial biogenesis respectively. FGF21 interacts with other molecules such as PPARα (peroxisome proliferator-activated receptor alpha) to exert its effects demonstrating its integration into broader metabolic networks. FGF21's interaction with these pathways highlights its function in energy utilization and storage.

FGF21 has connections to conditions such as obesity and type 2 diabetes. Its role in improving insulin sensitivity and promoting lipid oxidation makes it a molecule of interest in combating metabolic syndrome. FGF21 also has a relationship with hepatic proteins such as insulin receptor substrates (IRS) which play roles in liver functioning. Understanding these relationships offers insights into potential therapeutic strategies targeting metabolic disorders.

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Product protocols

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Target data

See full target information Fgf21

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nutrients 17: PubMed39861396

2025

Polyphenolic Hispolon Derived from Medicinal Mushrooms of the and Genera Promotes Wound Healing in Hyperglycemia-Induced Impairments.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Shan Liu,Mei-Chou Lai,Yu-Cheng Tzeng,I-Min Liu

Acta physiologica (Oxford, England) 238:e13935 PubMed36650072

2023

Rats lacking Ucp1 present a novel translational tool for the investigation of thermogenic adaptation during cold challenge.

Applications

Unspecified application

Species

Unspecified reactive species

Jaycob D Warfel,Carrie M Elks,David S Bayless,Bolormaa Vandanmagsar,Allison C Stone,Samuel E Velasquez,Paola Olivares-Nazar,Robert C Noland,Sujoy Ghosh,Jingying Zhang,Randall L Mynatt

Journal of molecular histology 52:905-918 PubMed34453661

2021

Elabela prevents angiotensin II-induced apoptosis and inflammation in rat aortic adventitial fibroblasts via the activation of FGF21-ACE2 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Juan-Juan Song,Mei Yang,Ying Liu,Jia-Wei Song,Xiao-Yan Liu,Ran Miao,Zhen-Zhou Zhang,Yu Liu,Yi-Fan Fan,Qian Zhang,Ying Dong,Xin-Chun Yang,Jiu-Chang Zhong

Experimental neurology 320:113007 PubMed31295445

2019

Delayed recanalization at 3 days after permanent MCAO attenuates neuronal apoptosis through FGF21/FGFR1/PI3K/Caspase-3 pathway in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Zheng,Nathanael Matei,Jinwei Pang,Xu Luo,Zhi Song,Jiping Tang,John H Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rat FGF-21 ELISA Kit

Sandwich ELISA - Rat FGF-21 ELISA Kit (AB223589)

Sandwich ELISA - Rat FGF-21 ELISA Kit (AB223589)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Primary Antibodies

Assay Kits

Primary Antibodies

Secondary Antibodies

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise