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AB108845

Rat Fibrinogen ELISA Kit

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Rat Fibrinogen ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Rat Fibrinogen in Rat in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.

View Alternative Names

Fibrinogen alpha chain, Fga

1 Images
Sandwich ELISA - Rat Fibrinogen ELISA Kit (AB108845)
  • sELISA

Supplier Data

Sandwich ELISA - Rat Fibrinogen ELISA Kit (AB108845)

Representative Standard Curve using ab108845

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Cell culture supernatant, Tissue Lysate, Urine

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 71 pg/mL

Range

0.098 - 6.25 ng/mL

Assay time

3h 30m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Fibrinogen Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Fibrinogen in rat urine, cell culture, cell lysate, and tissue samples.

A Fibrinogen specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Fibrinogen specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Fibrinogen captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.5" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108845-1x96Tests", "assetComponentDetails": [ { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108845-CMP04", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108845-CMP09", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108845-CMP08", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108845-CMP03", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108845-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Fibrinogen Microplate (12 x 8 well strips)", "number":"AB108845-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB108845-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Fibrinogen Standard", "number":"AB108845-CMP06", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Rat Fibrinogen Antibody", "number":"AB108845-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

Cleaved by the protease thrombin to yield monomers which, together with fibrinogen beta (FGB) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the immune response via both innate and T-cell mediated pathways.
See full target information Fga
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websiteProtocolBooklet
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Product promise

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