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AB108846

Rat Fibrinogen ELISA Kit

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Rat Fibrinogen ELISA Kit is a Competitive ELISA for the measurement of Rat Fibrinogen in Rat in Biofluids samples.

View Alternative Names

Ab1-181, Ab1-216, Ac1-581, Fgb, Fibrinogen beta chain, Liver regeneration-related protein LRRG036/LRRG043/LRRG189

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Competitive ELISA - Rat Fibrinogen ELISA Kit (AB108846)
  • cELISA

Supplier Data

Competitive ELISA - Rat Fibrinogen ELISA Kit (AB108846)

Representative Standard Curve using ab108846.

Key facts

Detection method

Colorimetric

Sample types

Plasma

Reacts with

Rat

Assay type

Competitive

Sensitivity

= 0.61 µg/mL

Range

0.781 - 50 µg/mL

Assay time

3h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Fibrinogen Rat in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Fibrinogen in plasma.

A Fibrinogen specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Fibrinogen is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Fibrinogen captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.9" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108846-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108846-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Fibrinogen Standard", "number":"AB108846-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"1X Biotinylated Rat Fibrinogen (Lyophilized)", "number":"AB108846-CMP02", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108846-CMP03", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108846-CMP07", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB108846-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Fibrinogen Microplate (12 x 8 well strips)", "number":"AB108846-CMP05", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108846-CMP09", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108846-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cleaved by the protease thrombin to yield monomers which, together with fibrinogen alpha (FGA) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the antibacterial immune response via both innate and T-cell mediated pathways.
See full target information Fgb
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Product
Assay Kits

AB108845

Rat Fibrinogen ELISA Kit

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rat-fibrinogen-elisa-kit-ab108845

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