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AB108850

Rat Fibronectin ELISA Kit

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(5 Publications)

Rat Fibronectin ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Rat Fibronectin in Rat in Biofluids samples.

View Alternative Names

Fibronectin, FN, Fn1

2 Images
Sandwich ELISA - Rat Fibronectin ELISA Kit (AB108850)
  • sELISA

Lab

Sandwich ELISA - Rat Fibronectin ELISA Kit (AB108850)

Fibronectin measured in various samples showing quantity (microgram) per mL of tested sample

Sandwich ELISA - Rat Fibronectin ELISA Kit (AB108850)
  • sELISA

Supplier Data

Sandwich ELISA - Rat Fibronectin ELISA Kit (AB108850)

Representative Standard Curve using ab108850.

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Serum

Reacts with

Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 69 pg/mL

Range

0.391 - 6.25 ng/mL

Assay time

4h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Fibronectin Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Fibronectin in plasma, serum, and urine.

A Fibronectin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Fibronectin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Fibronectin captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.8" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108850-1x96Tests", "assetComponentDetails": [ { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108850-CMP04", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108850-CMP07", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108850-CMP09", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Rat Fibronectin Standard", "number":"AB108850-CMP06", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Rat Fibronectin Antibody", "number":"AB108850-CMP02", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108850-CMP03", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108850-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Rat Fibronectin Microplate (12 x 8 well strips)", "number":"AB108850-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB108850-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (By similarity). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts (By similarity).. Anastellin. Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.. Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5 : ITGB1 integrin receptor signaling.
See full target information Fn1
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Food science & nutrition 11:1718-1727 PubMed37051364

2023

Effects of on hepatic and renal extracellular compounds turnover under diabetes in rat.

Applications

Unspecified application

Species

Unspecified reactive species

Maha Sayari,Houcine Dab,Mounira Tlili,Lazhar Zourgui,Abdelmajid Khabir

Experimental and therapeutic medicine 22:1017 PubMed34373703

2021

Ellagic acid inhibits high glucose-induced injury in rat mesangial cells via the PI3K/Akt/FOXO3a signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Lin,Guojian Liu,Xiaowen Kang,Ping Guo,Yu Shang,Ruomei Du,Xiyue Wang,Liting Chen,Rui Yue,Fanwu Kong,Qihan Zhu

International urology and nephrology 53:1247-1254 PubMed33942213

2021

Vitamin D protects glomerular mesangial cells from high glucose-induced injury by repressing JAK/STAT signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yiya Yang,Yuting Lei,Yumei Liang,Shuangshuang Fu,Congjun Yang,Kanghan Liu,Yinyin Chen

Frontiers in cellular and infection microbiology 9:466 PubMed31998665

2020

Comparative Analysis of the Host Response in a Rat Model of Deep-Partial and Full-Thickness Burn Wounds With Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Alan J Weaver,Kenneth S Brandenburg,Brian W Smith,Kai P Leung

Molecular medicine reports 16:7297-7304 PubMed28944869

2017

Promoting potential of adipose derived stem cells on peripheral nerve regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Jiayan Guo,Shu Guo,Yuxin Wang,Yanqiu Yu
View all publications
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